array:24 [
  "pii" => "S000173101100041X"
  "issn" => "00017310"
  "doi" => "10.1016/j.ad.2010.11.008"
  "estado" => "S300"
  "fechaPublicacion" => "2011-06-01"
  "aid" => "332"
  "copyright" => "Elsevier España, S.L. and AEDV"
  "copyrightAnyo" => "2010"
  "documento" => "simple-article"
  "crossmark" => 0
  "subdocumento" => "cor"
  "cita" => "Actas Dermosifiliogr. 2011;102:388-90"
  "abierto" => array:3 [
    "ES" => true
    "ES2" => true
    "LATM" => true
  ]
  "gratuito" => true
  "lecturas" => array:2 [
    "total" => 4188
    "formatos" => array:3 [
      "EPUB" => 42
      "HTML" => 3372
      "PDF" => 774
    ]
  ]
  "itemSiguiente" => array:18 [
    "pii" => "S0001731011000378"
    "issn" => "00017310"
    "doi" => "10.1016/j.ad.2010.07.010"
    "estado" => "S300"
    "fechaPublicacion" => "2011-06-01"
    "aid" => "328"
    "copyright" => "Elsevier España, S.L. y AEDV"
    "documento" => "simple-article"
    "crossmark" => 0
    "subdocumento" => "cor"
    "cita" => "Actas Dermosifiliogr. 2011;102:390-1"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 12820
      "formatos" => array:3 [
        "EPUB" => 3
        "HTML" => 9501
        "PDF" => 3316
      ]
    ]
    "es" => array:10 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Carta cient&#237;fico-cl&#237;nica</span>"
      "titulo" => "Rabdomi&#243;lisis durante el tratamiento con isotretino&#237;na"
      "tienePdf" => array:2 [
        0 => "es"
        1 => "en"
      ]
      "tieneTextoCompleto" => "es"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "390"
          "paginaFinal" => "391"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "en" => array:1 [
          "titulo" => "Rhabdomyolysis During Isotretinoin Therapy"
        ]
      ]
      "contieneTextoCompleto" => array:1 [
        "es" => true
      ]
      "contienePdf" => array:2 [
        "es" => true
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "S&#46; G&#243;mez-Bernal, L&#46; Rodr&#237;guez-Pazos, M&#46;T&#46; Rodr&#237;guez-Granados, J&#46; Toribio"
          "autores" => array:4 [
            0 => array:2 [
              "nombre" => "S&#46;"
              "apellidos" => "G&#243;mez-Bernal"
            ]
            1 => array:2 [
              "nombre" => "L&#46;"
              "apellidos" => "Rodr&#237;guez-Pazos"
            ]
            2 => array:2 [
              "nombre" => "M&#46;T&#46;"
              "apellidos" => "Rodr&#237;guez-Granados"
            ]
            3 => array:2 [
              "nombre" => "J&#46;"
              "apellidos" => "Toribio"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "es"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0001731011000378?idApp=UINPBA000044"
    "url" => "/00017310/0000010200000005/v1_201304241325/S0001731011000378/v1_201304241325/es/main.assets"
  ]
  "itemAnterior" => array:18 [
    "pii" => "S0001731011000093"
    "issn" => "00017310"
    "doi" => "10.1016/j.ad.2010.04.019"
    "estado" => "S300"
    "fechaPublicacion" => "2011-06-01"
    "aid" => "310"
    "copyright" => "Elsevier Espa&#241;a&#44; S&#46;L&#46; y AEDV"
    "documento" => "simple-article"
    "crossmark" => 0
    "subdocumento" => "cor"
    "cita" => "Actas Dermosifiliogr. 2011;102:387"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 3216
      "formatos" => array:3 [
        "EPUB" => 4
        "HTML" => 2531
        "PDF" => 681
      ]
    ]
    "es" => array:10 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Carta cient&#237;fico-cl&#237;nica</span>"
      "titulo" => "Reproducibilidad del diagn&#243;stico dermatol&#243;gico de onicomicosis en al &#225;mbito cl&#237;nico"
      "tienePdf" => array:2 [
        0 => "es"
        1 => "en"
      ]
      "tieneTextoCompleto" => "es"
      "paginas" => array:1 [
        0 => array:1 [
          "paginaInicial" => "387"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "en" => array:1 [
          "titulo" => "Reproducibility of the Diagnosis of Onychomycosis by Dermatologists in a Clinical Setting"
        ]
      ]
      "contieneTextoCompleto" => array:1 [
        "es" => true
      ]
      "contienePdf" => array:2 [
        "es" => true
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "M&#46; Cabanillas, I&#46; Garc&#237;a-Doval, B&#46; Monteagudo, O&#46; Su&#225;rez-Amor, F&#46; Allegue, L&#46; P&#233;rez-P&#233;rez, M&#46; Ginarte, J&#46; Labandeira, A&#46; Zulaica"
          "autores" => array:9 [
            0 => array:2 [
              "nombre" => "M&#46;"
              "apellidos" => "Cabanillas"
            ]
            1 => array:2 [
              "nombre" => "I&#46;"
              "apellidos" => "Garc&#237;a-Doval"
            ]
            2 => array:2 [
              "nombre" => "B&#46;"
              "apellidos" => "Monteagudo"
            ]
            3 => array:2 [
              "nombre" => "O&#46;"
              "apellidos" => "Su&#225;rez-Amor"
            ]
            4 => array:2 [
              "nombre" => "F&#46;"
              "apellidos" => "Allegue"
            ]
            5 => array:2 [
              "nombre" => "L&#46;"
              "apellidos" => "P&#233;rez-P&#233;rez"
            ]
            6 => array:2 [
              "nombre" => "M&#46;"
              "apellidos" => "Ginarte"
            ]
            7 => array:2 [
              "nombre" => "J&#46;"
              "apellidos" => "Labandeira"
            ]
            8 => array:2 [
              "nombre" => "A&#46;"
              "apellidos" => "Zulaica"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "es"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0001731011000093?idApp=UINPBA000044"
    "url" => "/00017310/0000010200000005/v1_201304241325/S0001731011000093/v1_201304241325/es/main.assets"
  ]
  "en" => array:15 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Case and Research Letters</span>"
    "titulo" => "CD25<span class="elsevierStyleSup">&#43;</span>CD8<span class="elsevierStyleSup">&#43;</span>&#44; CLA<span class="elsevierStyleSup">&#43;</span>CD4<span class="elsevierStyleSup">&#43;</span>&#44; CD11a<span class="elsevierStyleSup">&#43;</span> CD4<span class="elsevierStyleSup">&#43;</span>&#44; and CD11a<span class="elsevierStyleSup">&#43;</span> CD8<span class="elsevierStyleSup">&#43;</span> T Cell Counts Are Elevated in the Blood of Brazilian Patients With Chronic Plaque Psoriasis"
    "tieneTextoCompleto" => true
    "saludo" => "<span class="elsevierStyleItalic">To the Editor&#58;</span>"
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "388"
        "paginaFinal" => "390"
      ]
    ]
    "autores" => array:1 [
      0 => array:4 [
        "autoresLista" => "C&#46; Porto Ferreira, A&#46; Gomes da Silva, C&#46;J&#46; Martins, A&#46;M&#46; Da-Cruz"
        "autores" => array:4 [
          0 => array:4 [
            "nombre" => "C&#46;"
            "apellidos" => "Porto Ferreira"
            "email" => array:1 [
              0 => "drcassioferreira&#64;yahoo&#46;com&#46;br"
            ]
            "referencia" => array:3 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff0010"
              ]
              2 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">¿</span>"
                "identificador" => "cor0005"
              ]
            ]
          ]
          1 => array:3 [
            "nombre" => "A&#46;"
            "apellidos" => "Gomes da Silva"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
            ]
          ]
          2 => array:3 [
            "nombre" => "C&#46;J&#46;"
            "apellidos" => "Martins"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">c</span>"
                "identificador" => "aff0015"
              ]
            ]
          ]
          3 => array:3 [
            "nombre" => "A&#46;M&#46;"
            "apellidos" => "Da-Cruz"
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">d</span>"
                "identificador" => "aff0020"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:4 [
          0 => array:3 [
            "entidad" => "Laborat&#243;rio de Interdisciplinar de Pesquisas M&#233;dicas&#44; Instituto Oswaldo Cruz&#47;FIOCRUZ&#44; Brazil"
            "etiqueta" => "<span class="elsevierStyleSup">a</span>"
            "identificador" => "aff0005"
          ]
          1 => array:3 [
            "entidad" => "Instituto de Pesquisa Cl&#237;nica Evandro Chagas&#47;FIOCRUZ&#44; Brazil"
            "etiqueta" => "<span class="elsevierStyleSup">b</span>"
            "identificador" => "aff0010"
          ]
          2 => array:3 [
            "entidad" => "Servi&#231;o de Dermatologia&#44; Hospital Universit&#225;rio Gaffr&#233; e Guinle&#44; Universidade Federal do Estado do Rio de Janeiro &#40;UNIRIO&#41;&#44; Brazil"
            "etiqueta" => "<span class="elsevierStyleSup">c</span>"
            "identificador" => "aff0015"
          ]
          3 => array:3 [
            "entidad" => "Faculdade de Ci&#234;ncias M&#233;dicas&#44; Universidade do Estado do Rio de Janeiro&#44; Brazil"
            "etiqueta" => "<span class="elsevierStyleSup">d</span>"
            "identificador" => "aff0020"
          ]
        ]
        "correspondencia" => array:1 [
          0 => array:3 [
            "identificador" => "cor0005"
            "etiqueta" => "&#8270;"
            "correspondencia" => "Autor para correspondencia&#46;"
          ]
        ]
      ]
    ]
    "titulosAlternativos" => array:1 [
      "es" => array:1 [
        "titulo" => "Los linfocitos T CD25&#43;CD8&#43;&#44; CD4&#43;CLA&#43;&#44; CD11a&#43;CD4&#43; y CD11a&#43; CD8&#43; est&#225;n elevados en la sangre de los pacientes brasile&#241;os con psoriasis en placas cr&#243;nica"
      ]
    ]
    "resumenGrafico" => array:2 [
      "original" => 0
      "multimedia" => array:7 [
        "identificador" => "fig0010"
        "etiqueta" => "Figure 2"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr2.jpeg"
            "Alto" => 1403
            "Ancho" => 3312
            "Tamanyo" => 174098
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">A and B&#58; Assessment of the degree of activation of CD4<span class="elsevierStyleSup">&#43;</span> and CD8<span class="elsevierStyleSup">&#43;</span> T cells in psoriasis patients and controls&#46; Each point represents an individual&#46; The horizontal bar represent the median&#46;</p>"
        ]
      ]
    ]
    "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall">Psoriasis is a recurrent&#44; chronic inflammatory disorder that mainly affects the skin and joints<a class="elsevierStyleCrossRef" href="#bib0005"><span class="elsevierStyleSup">1</span></a>&#46; In view of the scarcity of studies on peripheral lymphocyte subpopulations in Brazilian psoriasis patients&#44; we performed cytofluorometric analysis of peripheral blood mononuclear cells &#40;PBMC&#41; in psoriatic patients and healthy controls in order to characterize the lymphocyte subpopulations and certain molecules involved in cell activation and migration&#46;</p><p id="par0010" class="elsevierStylePara elsevierViewall">The study was approved by the Ethics Committee of Escola de Medicina e Cirurgia &#40;UNIRIO&#44; MEC&#44; Brazil&#41; and all patients signed an informed consent&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">Twenty-five individuals were recruited from the Dermatology Department of HUGG&#47;UNIRIO&#47;MEC-Brazil&#46; Seventeen had chronic plaque psoriasis and there were 8 healthy controls&#46; None of the psoriasis patients was receiving systemic treatment&#46; The physician&#39;s global assessment score<a class="elsevierStyleCrossRefs" href="#bib0010"><span class="elsevierStyleSup">2&#8211;4</span></a> was 4 in 6 cases&#44; 5 in 6 cases&#44; and 6 in 5 cases&#46;</p><p id="par0020" class="elsevierStylePara elsevierViewall">A 20-ml blood sample was drawn from each patient and PBMCs were separated using the Ficoll-Hypaque gradient &#40;Sigma Chemical Co&#44; St&#46;Louis&#44; USA&#41;&#46; Five microliters of each monoclonal antibody&#8212;anti-CD3&#44; anti-CD4&#44; anti-CD8&#44; anti-CD11a &#40;Immunotech&#44; Beckman Coulter&#44; France&#41;&#44; anti-CD25&#44; anti-CD69&#44; and anti-CLA &#40;BD Biosciences&#44; California&#44; USA&#41;&#8212;were added according to the combinations listed in the <a class="elsevierStyleCrossRef" href="#tbl0005">table 1</a> table 1&#46; Laboratory procedures were performed according to the manufacturer&#39;s instructions<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a>&#46; The nonparametric Mann-Whitney and Kruskal-Wallis tests were used for the statistical analysis&#44; which was performed using GraphPad Prism version 4&#46;00 for Windows &#40;GraphPad Software&#44; San Diego&#44; California&#44; USA&#41;&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0025" class="elsevierStylePara elsevierViewall">Analysis of the lymphocyte subpopulations &#40;CD3<span class="elsevierStyleSup">&#43;</span> or total T cells&#44; CD4<span class="elsevierStyleSup">&#43;</span> or helper T cells&#44; and CD8<span class="elsevierStyleSup">&#43;</span> or cytotoxic T cells&#41; revealed no significant differences between patients with psoriasis and healthy controls&#46; The relative percentages of each lymphocyte subpopulation in patients and controls are shown in <a class="elsevierStyleCrossRef" href="#fig0005">Figure 1</a>&#46; Lymphocyte activation was determined through analysis of CD25 and CD69 expression &#40;<a class="elsevierStyleCrossRef" href="#fig0010">figs&#46; 2 A and B</a>&#41;&#46; Higher percentages of activated &#40;CD25<span class="elsevierStyleSup">&#43;</span> and CD69<span class="elsevierStyleSup">&#43;</span>&#41; cells were detected in the 2 lymphocyte subpopulations &#40;CD4<span class="elsevierStyleSup">&#43;</span> and CD8<span class="elsevierStyleSup">&#43;</span>&#41; in psoriatic patients&#59; the difference compared to controls was significant for the percentage of CD25<span class="elsevierStyleSup">&#43;</span> cells in the CD8<span class="elsevierStyleSup">&#43;</span> T-cell subpopulation &#40;mean &#91;SD&#93;&#44; 39&#46;2&#37; &#40;26&#46;9&#41;&#59; median&#44; 35&#46;1&#37;&#59; <span class="elsevierStyleItalic">P</span> &#60;&#46;05&#41;&#44; and there was a trend to elevation of the CD25<span class="elsevierStyleSup">&#43;</span>&#160;CD4<span class="elsevierStyleSup">&#43;</span> subpopulation&#46; There was also a trend to elevation in the percentage of CD69<span class="elsevierStyleSup">&#43;</span> cells in both T-cell subpopulations &#40;CD4<span class="elsevierStyleSup">&#43;</span>&#58; mean&#44; 16&#46;0&#37; &#91;19&#46;6&#93;&#59; median&#44; 7&#46;8&#37;&#59; CD8<span class="elsevierStyleSup">&#43;</span>&#58; mean&#44; 2&#46;0&#37; &#91;1&#46;47&#93;&#59; median&#44; 2&#46;23&#37;&#41; in psoriatic patients when compared to controls &#40;<a class="elsevierStyleCrossRef" href="#fig0010">fig&#46; 2</a>B&#41;&#46; Migration of circulating T lymphocytes to the skin was studied through an analysis of CLA and CD11a expression&#46; Compared to control subjects&#44; patients with psoriasis presented an increase in the percentage of CD4<span class="elsevierStyleSup">&#43;</span> T cells expressing CLA &#40;control group&#58; mean&#44; 19&#46;30&#37; &#91;13&#46;13&#93;&#59; median&#44; 14&#46;67&#37;&#59; psoriasis group&#58; mean&#44; 38&#46;86&#37; &#91;20&#46;67&#93;&#59; median&#44; 39&#46;76&#37;&#59; <span class="elsevierStyleItalic">P</span>&#60;&#46;05&#41;&#46; However&#44; this was not observed in the CD8<span class="elsevierStyleSup">&#43;</span>&#160;T cells&#44; although increased CLA expression was detected in 4 of the 10 patients with high levels of CD8<span class="elsevierStyleSup">&#43;</span> lymphocytes &#40;<a class="elsevierStyleCrossRef" href="#fig0015">fig&#46; 3</a>A&#41;&#46; A significant increase in the percentage of cells with CD11a expression was observed in psoriasis patients compared to controls in both CD4<span class="elsevierStyleSup">&#43;</span> &#40;mean&#44; 82&#46;17&#37; &#91;29&#46;91&#93;&#59; median&#44; 99&#46;45&#37;&#59; <span class="elsevierStyleItalic">P</span> &#60; &#46;01&#41; and CD8<span class="elsevierStyleSup">&#43;</span> T cells &#40;mean&#44; 84&#46;96 &#91;29&#46;77&#93;&#59; median&#44; 99&#46;75&#59; <span class="elsevierStyleItalic">P</span> &#60; &#46;05&#41; &#40;<a class="elsevierStyleCrossRef" href="#fig0015">fig&#46; 3</a>B&#41;&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><elsevierMultimedia ident="fig0010"></elsevierMultimedia><elsevierMultimedia ident="fig0015"></elsevierMultimedia><p id="par0030" class="elsevierStylePara elsevierViewall">Although our study is somewhat limited by the small sample size&#44; the results showed that there was no change in the proportion of CD4<span class="elsevierStyleSup">&#43;</span> and CD8<span class="elsevierStyleSup">&#43;</span> T cells in the PBMC population in psoriatic patients when compared with general population&#44; as has been reported in previous studies<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a>&#46; However&#44; we observed qualitative differences between the 2 groups in the expression of activation molecules CD25 and CD69&#46; Although the results were significant only for CD25 expression in CD8<span class="elsevierStyleSup">&#43;</span> T cells&#44; there was a trend to increased expression of CD25 and CD69 in both CD4<span class="elsevierStyleSup">&#43;</span> and CD8<span class="elsevierStyleSup">&#43;</span> T cells&#44; findings not reported by other authors<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a>&#46; The presence of activation molecules has been detected in the initial stages of psoriasis&#44; even prior to the onset of clinically apparent lesions&#59; these molecules are therefore presumably involved in lymphocyte recruitment and migration<a class="elsevierStyleCrossRefs" href="#bib0040"><span class="elsevierStyleSup">8&#44;9</span></a>&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">Our data indicate that&#44; although there is no significant increase or variation in the relative percentages of circulating mononuclear cells in psoriatic patients&#44; these cells are qualitatively different because they express activation molecules that are involved in the initiation and progression of psoriasis lesions&#46;</p></span>"
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "multimedia" => array:4 [
      0 => array:7 [
        "identificador" => "fig0005"
        "etiqueta" => "Figure 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 1403
            "Ancho" => 1639
            "Tamanyo" => 98700
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Quantification of T lymphocyte subpopulations &#40;CD4<span class="elsevierStyleSup">&#43;</span> and CD8<span class="elsevierStyleSup">&#43;</span> T cells&#41; in the peripheral blood of patients with chronic plaque psoriasis&#46; The results are expressed as percentage of positive cells&#46; The horizontal bars represent the median values of the results&#46; Each point represents an individual&#46;</p>"
        ]
      ]
      1 => array:7 [
        "identificador" => "fig0010"
        "etiqueta" => "Figure 2"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr2.jpeg"
            "Alto" => 1403
            "Ancho" => 3312
            "Tamanyo" => 174098
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">A and B&#58; Assessment of the degree of activation of CD4<span class="elsevierStyleSup">&#43;</span> and CD8<span class="elsevierStyleSup">&#43;</span> T cells in psoriasis patients and controls&#46; Each point represents an individual&#46; The horizontal bar represent the median&#46;</p>"
        ]
      ]
      2 => array:7 [
        "identificador" => "fig0015"
        "etiqueta" => "Figure 3"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr3.jpeg"
            "Alto" => 1271
            "Ancho" => 3375
            "Tamanyo" => 192480
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">A&#46; Elevated expression of molecules related to cutaneous cell migration &#40;cutaneous lymphocyte associated antigen &#91;CLA&#93;&#41; B&#46; Endothelial tissue inflammation &#40;CD11a&#41; in CD4<span class="elsevierStyleSup">&#43;</span> and CD8<span class="elsevierStyleSup">&#43;</span> T cells in psoriasis patients and controls&#46; Each point represents an individual&#46; The horizontal bars represent the median values&#46;</p>"
        ]
      ]
      3 => array:7 [
        "identificador" => "tbl0005"
        "etiqueta" => "Table 1"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:1 [
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">Monoclonal markers&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " colspan="3" align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">Analysis</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">Source&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD3-PC5&#47;CD4-PE&#47;CD8-FITC&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD3 in total lymphocytes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD4 into CD3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD8 into CD3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Immunotech&#44; Beckman Coulter&#44; France&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD4-PC5&#47;CD8-FITC&#47;CD25-PE&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD25 in total lymphocytes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD25 into CD4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD25 into CD8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">BD Biosciences&#44; CA&#44; USA&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD4-PC5&#47;CD8-PE&#47;CD69-FITC&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD69 in total lymphocytes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD69 into CD4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD69 into CD8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">BD Biosciences&#44; CA&#44; USA&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD4-PC5&#47;CD8-PE&#47;CLA-FITC&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CLA in total lymphocytes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CLA into CD4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CLA into CD8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">BD Biosciences&#44; CA&#44; USA&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD4-PC5&#47;CD8-PE&#47;CD11a-FITC&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD11a in total lymphocytes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD11a into CD4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">CD11a into CD8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Immunotech&#44; Beckman Coulter&#44; France&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab182362.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Set of monoclonal markers</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0005"
          "bibliografiaReferencia" => array:9 [
            0 => array:3 [
              "identificador" => "bib0005"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Psoriasis&#8211;epidemiology and clinical spectrum"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "E&#46; Christophers"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Exp Dermatol&#46;"
                        "fecha" => "2001"
                        "volumen" => "26"
                        "paginaInicial" => "314"
                        "paginaFinal" => "320"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11422182"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0010"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Documento de consenso sobre la evaluaci&#243;n y el tratamiento de la psoriasis moderada&#47;grave del Grupo Espa&#241;ol de Psoriasis de la Academia Espa&#241;ola de Dermatolog&#237;a y Venereolog&#237;a"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "L&#46; Puig"
                            1 => "X&#46; Bordas"
                            2 => "J&#46;M&#46; Carrascosa"
                            3 => "E&#46; Daud&#233;n"
                            4 => "C&#46; Ferr&#225;ndiz"
                            5 => "J&#46;M&#46; Hernanz"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Actas Dermosifiliogr&#46;"
                        "fecha" => "2009"
                        "volumen" => "100"
                        "paginaInicial" => "277"
                        "paginaFinal" => "286"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19463230"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0015"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The National Psoriasis Foundation Psoriasis Score &#40;NPF-PS&#41; System versus the Psoriasis Area Severity Index &#40;PASI&#41; and Physician&#39;s Global Assessment &#40;PGA&#41;&#58; a comparison"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "A&#46;B&#46; Gottlieb"
                            1 => "U&#46; Chaudhari"
                            2 => "D&#46;G&#46; Baker"
                            3 => "M&#46; Perate"
                            4 => "L&#46;T&#46; Dooley"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Drugs Dermatol&#46;"
                        "fecha" => "2003"
                        "volumen" => "2"
                        "paginaInicial" => "260"
                        "paginaFinal" => "266"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12848110"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib0020"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Psoriasis affects individuals of African descent and white Brazilians similarly"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "C&#46; Porto Ferreira"
                            1 => "C&#46;J&#46; Martins"
                            2 => "P&#46;R&#46; Issa"
                            3 => "R&#46; de Vasconcellos Carvalhaes de Oliveira"
                            4 => "A&#46;M&#46; Da-Cruz"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Actas Dermosifiliogr&#46;"
                        "fecha" => "2010"
                        "volumen" => "101"
                        "paginaInicial" => "230"
                        "paginaFinal" => "234"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20398598"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0025"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Flow cytometry instrumentation in research and clinical laboratories"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "L&#46;D&#46; Bogh"
                            1 => "T&#46;A&#46; Duling"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Lab Sci&#46;"
                        "fecha" => "1993"
                        "volumen" => "6"
                        "paginaInicial" => "167"
                        "paginaFinal" => "173"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10146217"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0030"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "CD3&#43;CD56&#43;NK T cells are significantly decreased in the peripheral blood of patientswith psoriasis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "A&#46; Koreck"
                            1 => "A&#46; Sur&#225;nyi"
                            2 => "B&#46;J&#46; Sz&#246;ny"
                            3 => "&#193;&#46; Farkas"
                            4 => "Z&#46; Bata-Cs&#246;rg&#246;"
                            5 => "L&#46; Kem&#233;ny"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Exp Immunol&#46;"
                        "fecha" => "2002"
                        "volumen" => "127"
                        "paginaInicial" => "176"
                        "paginaFinal" => "182"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11882050"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib0035"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The frequency of CLA&#43; CD8&#43; T cells in the blood of psoriasis patients correlates closely with the severity of their disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "H&#46; Sigmundsdottir"
                            1 => "J&#46;E&#46; Gudjonsson"
                            2 => "I&#46; Jonsdottir"
                            3 => "B&#46;R&#46; Ludviksson"
                            4 => "H&#46; Valdimarsson"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Exp Immunol&#46;"
                        "fecha" => "2001"
                        "volumen" => "126"
                        "paginaInicial" => "365"
                        "paginaFinal" => "369"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11703383"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib0040"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "CLA&#43; T cells in cutaneous disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "L&#46;F&#46; Santamar&#237;a-Babi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "Eur J Dermatol&#46;"
                        "fecha" => "2004"
                        "volumen" => "15"
                        "paginaInicial" => "13"
                        "paginaFinal" => "18"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15701587"
                            "web" => "Medline"
                          ]
                        ]
                        "itemHostRev" => array:3 [
                          "pii" => "S1474442205702842"
                          "estado" => "S300"
                          "issn" => "14744422"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib0045"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Homing receptor and chemokine receptor on intraepidermal T cells in psoriasis vulgaris"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "Y&#46; Teraki"
                            1 => "A&#46; Miyake"
                            2 => "R&#46; Takebayashi"
                            3 => "T&#46; Shiohara"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/j.1365-2230.2004.01638.x"
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Exp Dermatol&#46;"
                        "fecha" => "2004"
                        "volumen" => "29"
                        "paginaInicial" => "658"
                        "paginaFinal" => "663"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15550147"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
    "agradecimientos" => array:1 [
      0 => array:3 [
        "identificador" => "xack35246"
        "titulo" => "Acknowledgments"
        "texto" => "<p id="par0040" class="elsevierStylePara elsevierViewall">We are grateful to Ana Cristina de Almeida for her valuable suggestions&#44; to Gustavo Estef Lino da Silveira for the linguistic revision&#44; and to the medical staff of Servi&#231;o de Dermatologia-HUGG&#47;UNIRIO&#44; Rio de Janeiro&#44; Brazil&#46; C Porto-Ferreira is a graduate student at Programa de P&#243;s-Gradua&#231;&#227;o em Ci&#234;ncias M&#233;dicas&#44; FCM-UERJ&#44; Rio de Janeiro&#44; Brazil&#46; AM Da-Cruz is a research fellow from FAPERJ&#44; Rio de Janeiro&#44; Brazil&#46;</p>"
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/00017310/0000010200000005/v1_201304241325/S000173101100041X/v1_201304241325/en/main.assets"
  "Apartado" => array:4 [
    "identificador" => "6143"
    "tipo" => "SECCION"
    "en" => array:2 [
      "titulo" => "Cartas cient&#237;fico-cl&#237;nicas"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "en"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/00017310/0000010200000005/v1_201304241325/S000173101100041X/v1_201304241325/en/main.pdf?idApp=UINPBA000044&text.app=https://actasdermo.org/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S000173101100041X?idApp=UINPBA000044"
  "pdfAlternativo" => array:1 [
    0 => array:3 [
      "idioma" => "es"
      "documentoPdf" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/file/00017310/addon/S000173101100041X/S300/es/103v102n05a90014935pdf001_2.pdf"
      "portada" => "103v102n05a90014935pdf001_2.png"
    ]
  ]
]
Compartir
Información de la revista
Vol. 102. Núm. 5.
Páginas 388-390 (junio 2011)
Compartir
Compartir
Descargar PDF
Spanish PDF
Más opciones de artículo
Vol. 102. Núm. 5.
Páginas 388-390 (junio 2011)
Case and Research Letters
Acceso a texto completo
CD25+CD8+, CLA+CD4+, CD11a+ CD4+, and CD11a+ CD8+ T Cell Counts Are Elevated in the Blood of Brazilian Patients With Chronic Plaque Psoriasis
Los linfocitos T CD25+CD8+, CD4+CLA+, CD11a+CD4+ y CD11a+ CD8+ están elevados en la sangre de los pacientes brasileños con psoriasis en placas crónica
Visitas
7985
C. Porto Ferreiraa,b,
Autor para correspondencia
drcassioferreira@yahoo.com.br

Autor para correspondencia.
, A. Gomes da Silvaa, C.J. Martinsc, A.M. Da-Cruza,d
a Laboratório de Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz/FIOCRUZ, Brazil
b Instituto de Pesquisa Clínica Evandro Chagas/FIOCRUZ, Brazil
c Serviço de Dermatologia, Hospital Universitário Gaffré e Guinle, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Brazil
d Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Brazil
Este artículo ha recibido
Información del artículo
Texto completo
Bibliografía
Descargar PDF
Estadísticas
Figuras (3)
Mostrar másMostrar menos
Tablas (1)
Table 1. Set of monoclonal markers
Texto completo
To the Editor:

Psoriasis is a recurrent, chronic inflammatory disorder that mainly affects the skin and joints1. In view of the scarcity of studies on peripheral lymphocyte subpopulations in Brazilian psoriasis patients, we performed cytofluorometric analysis of peripheral blood mononuclear cells (PBMC) in psoriatic patients and healthy controls in order to characterize the lymphocyte subpopulations and certain molecules involved in cell activation and migration.

The study was approved by the Ethics Committee of Escola de Medicina e Cirurgia (UNIRIO, MEC, Brazil) and all patients signed an informed consent.

Twenty-five individuals were recruited from the Dermatology Department of HUGG/UNIRIO/MEC-Brazil. Seventeen had chronic plaque psoriasis and there were 8 healthy controls. None of the psoriasis patients was receiving systemic treatment. The physician's global assessment score2–4 was 4 in 6 cases, 5 in 6 cases, and 6 in 5 cases.

A 20-ml blood sample was drawn from each patient and PBMCs were separated using the Ficoll-Hypaque gradient (Sigma Chemical Co, St.Louis, USA). Five microliters of each monoclonal antibody—anti-CD3, anti-CD4, anti-CD8, anti-CD11a (Immunotech, Beckman Coulter, France), anti-CD25, anti-CD69, and anti-CLA (BD Biosciences, California, USA)—were added according to the combinations listed in the table 1 table 1. Laboratory procedures were performed according to the manufacturer's instructions5. The nonparametric Mann-Whitney and Kruskal-Wallis tests were used for the statistical analysis, which was performed using GraphPad Prism version 4.00 for Windows (GraphPad Software, San Diego, California, USA).

Table 1.

Set of monoclonal markers

Monoclonal markers  AnalysisSource 
CD3-PC5/CD4-PE/CD8-FITC  CD3 in total lymphocytes  CD4 into CD3  CD8 into CD3  Immunotech, Beckman Coulter, France 
CD4-PC5/CD8-FITC/CD25-PE  CD25 in total lymphocytes  CD25 into CD4  CD25 into CD8  BD Biosciences, CA, USA 
CD4-PC5/CD8-PE/CD69-FITC  CD69 in total lymphocytes  CD69 into CD4  CD69 into CD8  BD Biosciences, CA, USA 
CD4-PC5/CD8-PE/CLA-FITC  CLA in total lymphocytes  CLA into CD4  CLA into CD8  BD Biosciences, CA, USA 
CD4-PC5/CD8-PE/CD11a-FITC  CD11a in total lymphocytes  CD11a into CD4  CD11a into CD8  Immunotech, Beckman Coulter, France 

Analysis of the lymphocyte subpopulations (CD3+ or total T cells, CD4+ or helper T cells, and CD8+ or cytotoxic T cells) revealed no significant differences between patients with psoriasis and healthy controls. The relative percentages of each lymphocyte subpopulation in patients and controls are shown in Figure 1. Lymphocyte activation was determined through analysis of CD25 and CD69 expression (figs. 2 A and B). Higher percentages of activated (CD25+ and CD69+) cells were detected in the 2 lymphocyte subpopulations (CD4+ and CD8+) in psoriatic patients; the difference compared to controls was significant for the percentage of CD25+ cells in the CD8+ T-cell subpopulation (mean [SD], 39.2% (26.9); median, 35.1%; P <.05), and there was a trend to elevation of the CD25+ CD4+ subpopulation. There was also a trend to elevation in the percentage of CD69+ cells in both T-cell subpopulations (CD4+: mean, 16.0% [19.6]; median, 7.8%; CD8+: mean, 2.0% [1.47]; median, 2.23%) in psoriatic patients when compared to controls (fig. 2B). Migration of circulating T lymphocytes to the skin was studied through an analysis of CLA and CD11a expression. Compared to control subjects, patients with psoriasis presented an increase in the percentage of CD4+ T cells expressing CLA (control group: mean, 19.30% [13.13]; median, 14.67%; psoriasis group: mean, 38.86% [20.67]; median, 39.76%; P<.05). However, this was not observed in the CD8+ T cells, although increased CLA expression was detected in 4 of the 10 patients with high levels of CD8+ lymphocytes (fig. 3A). A significant increase in the percentage of cells with CD11a expression was observed in psoriasis patients compared to controls in both CD4+ (mean, 82.17% [29.91]; median, 99.45%; P < .01) and CD8+ T cells (mean, 84.96 [29.77]; median, 99.75; P < .05) (fig. 3B).

Figure 1.

Quantification of T lymphocyte subpopulations (CD4+ and CD8+ T cells) in the peripheral blood of patients with chronic plaque psoriasis. The results are expressed as percentage of positive cells. The horizontal bars represent the median values of the results. Each point represents an individual.

(0.09MB).
Figure 2.

A and B: Assessment of the degree of activation of CD4+ and CD8+ T cells in psoriasis patients and controls. Each point represents an individual. The horizontal bar represent the median.

(0.17MB).
Figure 3.

A. Elevated expression of molecules related to cutaneous cell migration (cutaneous lymphocyte associated antigen [CLA]) B. Endothelial tissue inflammation (CD11a) in CD4+ and CD8+ T cells in psoriasis patients and controls. Each point represents an individual. The horizontal bars represent the median values.

(0.18MB).

Although our study is somewhat limited by the small sample size, the results showed that there was no change in the proportion of CD4+ and CD8+ T cells in the PBMC population in psoriatic patients when compared with general population, as has been reported in previous studies6. However, we observed qualitative differences between the 2 groups in the expression of activation molecules CD25 and CD69. Although the results were significant only for CD25 expression in CD8+ T cells, there was a trend to increased expression of CD25 and CD69 in both CD4+ and CD8+ T cells, findings not reported by other authors7. The presence of activation molecules has been detected in the initial stages of psoriasis, even prior to the onset of clinically apparent lesions; these molecules are therefore presumably involved in lymphocyte recruitment and migration8,9.

Our data indicate that, although there is no significant increase or variation in the relative percentages of circulating mononuclear cells in psoriatic patients, these cells are qualitatively different because they express activation molecules that are involved in the initiation and progression of psoriasis lesions.

Acknowledgments

We are grateful to Ana Cristina de Almeida for her valuable suggestions, to Gustavo Estef Lino da Silveira for the linguistic revision, and to the medical staff of Serviço de Dermatologia-HUGG/UNIRIO, Rio de Janeiro, Brazil. C Porto-Ferreira is a graduate student at Programa de Pós-Graduação em Ciências Médicas, FCM-UERJ, Rio de Janeiro, Brazil. AM Da-Cruz is a research fellow from FAPERJ, Rio de Janeiro, Brazil.

References
[1]
E. Christophers.
Psoriasis–epidemiology and clinical spectrum.
Clin Exp Dermatol., 26 (2001), pp. 314-320
[2]
L. Puig, X. Bordas, J.M. Carrascosa, E. Daudén, C. Ferrándiz, J.M. Hernanz, et al.
Documento de consenso sobre la evaluación y el tratamiento de la psoriasis moderada/grave del Grupo Español de Psoriasis de la Academia Española de Dermatología y Venereología.
Actas Dermosifiliogr., 100 (2009), pp. 277-286
[3]
A.B. Gottlieb, U. Chaudhari, D.G. Baker, M. Perate, L.T. Dooley.
The National Psoriasis Foundation Psoriasis Score (NPF-PS) System versus the Psoriasis Area Severity Index (PASI) and Physician's Global Assessment (PGA): a comparison.
J Drugs Dermatol., 2 (2003), pp. 260-266
[4]
C. Porto Ferreira, C.J. Martins, P.R. Issa, R. de Vasconcellos Carvalhaes de Oliveira, A.M. Da-Cruz.
Psoriasis affects individuals of African descent and white Brazilians similarly.
Actas Dermosifiliogr., 101 (2010), pp. 230-234
[5]
L.D. Bogh, T.A. Duling.
Flow cytometry instrumentation in research and clinical laboratories.
Clin Lab Sci., 6 (1993), pp. 167-173
[6]
A. Koreck, A. Surányi, B.J. Szöny, Á. Farkas, Z. Bata-Csörgö, L. Kemény, et al.
CD3+CD56+NK T cells are significantly decreased in the peripheral blood of patientswith psoriasis.
Clin Exp Immunol., 127 (2002), pp. 176-182
[7]
H. Sigmundsdottir, J.E. Gudjonsson, I. Jonsdottir, B.R. Ludviksson, H. Valdimarsson.
The frequency of CLA+ CD8+ T cells in the blood of psoriasis patients correlates closely with the severity of their disease.
Clin Exp Immunol., 126 (2001), pp. 365-369
[8]
L.F. Santamaría-Babi.
CLA+ T cells in cutaneous disease.
Eur J Dermatol., 15 (2004), pp. 13-18
[9]
Y. Teraki, A. Miyake, R. Takebayashi, T. Shiohara.
Homing receptor and chemokine receptor on intraepidermal T cells in psoriasis vulgaris.
Clin Exp Dermatol., 29 (2004), pp. 658-663
Copyright © 2010. Elsevier España, S.L. and AEDV
Descargar PDF
Idiomas
Actas Dermo-Sifiliográficas
Opciones de artículo
Herramientas
es en

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?