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        "titulo" => "Abstract"
        "resumen" => "<span class="elsevierStyleSectionTitle">Introduction</span><p class="elsevierStyleSimplePara elsevierViewall">Epidermodysplasia verruciformis &#40;EV&#41; is associated with greater susceptibility to infection by certain oncogenic subtypes of human papillomavirus &#40;HPV&#41;&#46; Among other histologic findings&#44; large&#44; clear&#44; oval or rounded cells &#40;EV cells&#41; are observed in the granular layer in EV&#44; and some authors consider these cells to be markers of immunosuppression&#46;</p> <span class="elsevierStyleSectionTitle">Material and Methods</span><p class="elsevierStyleSimplePara elsevierViewall">We analyzed 229 squamous cell carcinomas &#40;SCC&#41; to determine whether EV cells were present and to assess whether their presence was associated either with localized or cutaneous immunosuppression &#40;tumors with signs of severe chronic actinic damage or severe stasis dermatitis&#41; or with systemic immunosuppression &#40;immunocompromised or elderly patients&#41;&#46;</p> <span class="elsevierStyleSectionTitle">Results</span><p class="elsevierStyleSimplePara elsevierViewall">We observed EV cells in 33 SCC&#46; No statistically significant relationship was observed between the presence of EV cells and immunosuppression&#46; We performed polymerase chain reaction in 8 lesions&#44; but the results were not informative as the DNA was denatured&#46;</p> <span class="elsevierStyleSectionTitle">Conclusions</span><p class="elsevierStyleSimplePara elsevierViewall">We found no relationship between the presence of EV cells and localized or systemic immunosuppression&#44; possibly because the sample was inadequate &#40;almost all SCC studied were associated with signs of immunosuppression&#44; irrespective of the presence or absence of EV cells&#41;&#46; Further studies will be required to compare lesions associated with immunosuppression with those in which immunosuppression is absent&#46; The presence of EV cells may be the result of cytopathic effects of certain HPV subtypes&#44; such as HPV 5 or 8&#44; but this will need to be demonstrated using techniques such as polymerase chain reaction&#46;</p>"
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        "resumen" => "<span class="elsevierStyleSectionTitle">Introducci&#243;n</span><p class="elsevierStyleSimplePara elsevierViewall">La epidermodisplasia verruciforme &#40;EV&#41; es una enfermedad en la que existe una mayor susceptibilidad gen&#233;tica a la infecci&#243;n por determinados subtipos oncog&#233;nicos del virus del papiloma humano &#40;VPH&#41;&#46; Entre otros hallazgos histol&#243;gicos&#44; en la EV suelen observarse unas c&#233;lulas claras&#44; grandes&#44; ovaladas o redondeadas &#40;c&#233;lulas EV&#41; en la capa granulosa&#44; que seg&#250;n algunos autores se podr&#237;an considerar como un marcador histol&#243;gico de inmunodepresi&#243;n&#46;</p> <span class="elsevierStyleSectionTitle">Material y m&#233;todos</span><p class="elsevierStyleSimplePara elsevierViewall">Hemos estudiado 229 carcinomas espinocelulares &#40;CE&#41; para valorar si presentaban c&#233;lulas EV y si se relacionaban con inmunodepresi&#243;n&#44; tanto localizada o cut&#225;nea &#40;tumores con signos de intenso da&#241;o solar cr&#243;nico o con una dermatitis de estasis intensa&#41;&#44; como sist&#233;mica &#40;pacientes inmunodeprimidos o ancianos&#41;&#46;</p> <span class="elsevierStyleSectionTitle">Resultados</span><p class="elsevierStyleSimplePara elsevierViewall">Observamos c&#233;lulas EV en 33 carcinomas&#46; No hemos encontrado relaci&#243;n significativa entre la existencia de c&#233;lulas EV e inmunodepresi&#243;n&#46; Realizamos reacci&#243;n en cadena de la polimerasa &#40;PCR&#41; en 8 lesiones&#44; pero los resultados no fueron valorables porque el ADN estaba desnaturalizado&#46;</p> <span class="elsevierStyleSectionTitle">Conclusiones</span><p class="elsevierStyleSimplePara elsevierViewall">No hemos demostrado relaci&#243;n entre las c&#233;lulas EV e inmunodepresi&#243;n&#44; localizada o sist&#233;mica&#44; posiblemente porque la muestra no era adecuada &#40;se podr&#237;a considerar que pr&#225;cticamente todos los CE estudiados se asociaban a signos de inmunodepresi&#243;n&#44; tanto aquellos que presentaban c&#233;lulas EV como los que no&#41;&#46; Ser&#237;an necesarios m&#225;s estudios que comparasen lesiones asociadas a inmunodepresi&#243;n con otras que no&#46; Es posible que estas c&#233;lulas EV sean el resultado de los efectos citop&#225;ticos de determinados subtipos de VPH&#44; como el VPH-5 o el VPH-8&#44; aunque ser&#237;a necesario demostrar esta hip&#243;tesis mediante t&#233;cnicas de PCR o similar&#46;</p>"
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Vol. 99. Núm. 4.
Páginas 269-274 (mayo 2008)
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Vol. 99. Núm. 4.
Páginas 269-274 (mayo 2008)
Original articles
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Epidermodysplasia Verruciformis-Like Cells as Histologic Markers of Immunosuppression: Review of 229 Squamous Cell Carcinomas*
Las Células Tipo Epidermodisplasia Verruciforme Como Marcador Histológico de Inmunodepresión: Revisión de 229 Carcinomas Espinocelulares
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R. Corbalán-Véleza,
Autor para correspondencia
raulcorb@gmail.com

Correspondence: Servicio de Dermatología, Hospital Universitario Virgen de la Arrixaca, Ctra. Murcia-Cartagena, s/n, 30120 El Palmar, Murcia, Spain.
, J.A. Ruiz-Maciáb, C. Brufauc, J.M. López-Lozanod, E. Martínez-Barbae, J. Martínez-Escribanoa, F.J. Carapetof
a Servicio de Dermatología, Hospital Universitario Virgen de la Arrixaca, Murcia, Spain
b Servicio de Anatomía Patológica, Hospital Vega Baja, Orihuela, Alicante, Spain
c Servicio de Dermatología, Hospital General Reina Sofía, Murcia, Spain
d Servicio de Medicina Preventiva, Hospital Vega Baja, Orihuela, Alicante, Spain
e Servicio de Anatomía Patológica, Hospital Universitario Virgen de la Arrixaca, Murcia, Spain
f Servicio de Dermatología, Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain*
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Abstract
Introduction

Epidermodysplasia verruciformis (EV) is associated with greater susceptibility to infection by certain oncogenic subtypes of human papillomavirus (HPV). Among other histologic findings, large, clear, oval or rounded cells (EV cells) are observed in the granular layer in EV, and some authors consider these cells to be markers of immunosuppression.

Material and Methods

We analyzed 229 squamous cell carcinomas (SCC) to determine whether EV cells were present and to assess whether their presence was associated either with localized or cutaneous immunosuppression (tumors with signs of severe chronic actinic damage or severe stasis dermatitis) or with systemic immunosuppression (immunocompromised or elderly patients).

Results

We observed EV cells in 33 SCC. No statistically significant relationship was observed between the presence of EV cells and immunosuppression. We performed polymerase chain reaction in 8 lesions, but the results were not informative as the DNA was denatured.

Conclusions

We found no relationship between the presence of EV cells and localized or systemic immunosuppression, possibly because the sample was inadequate (almost all SCC studied were associated with signs of immunosuppression, irrespective of the presence or absence of EV cells). Further studies will be required to compare lesions associated with immunosuppression with those in which immunosuppression is absent. The presence of EV cells may be the result of cytopathic effects of certain HPV subtypes, such as HPV 5 or 8, but this will need to be demonstrated using techniques such as polymerase chain reaction.

Key words:
epidermodysplasia verruciformis
immunosuppression
papillomavirus
solar elastosis
Resumen
Introducción

La epidermodisplasia verruciforme (EV) es una enfermedad en la que existe una mayor susceptibilidad genética a la infección por determinados subtipos oncogénicos del virus del papiloma humano (VPH). Entre otros hallazgos histológicos, en la EV suelen observarse unas células claras, grandes, ovaladas o redondeadas (células EV) en la capa granulosa, que según algunos autores se podrían considerar como un marcador histológico de inmunodepresión.

Material y métodos

Hemos estudiado 229 carcinomas espinocelulares (CE) para valorar si presentaban células EV y si se relacionaban con inmunodepresión, tanto localizada o cutánea (tumores con signos de intenso daño solar crónico o con una dermatitis de estasis intensa), como sistémica (pacientes inmunodeprimidos o ancianos).

Resultados

Observamos células EV en 33 carcinomas. No hemos encontrado relación significativa entre la existencia de células EV e inmunodepresión. Realizamos reacción en cadena de la polimerasa (PCR) en 8 lesiones, pero los resultados no fueron valorables porque el ADN estaba desnaturalizado.

Conclusiones

No hemos demostrado relación entre las células EV e inmunodepresión, localizada o sistémica, posiblemente porque la muestra no era adecuada (se podría considerar que prácticamente todos los CE estudiados se asociaban a signos de inmunodepresión, tanto aquellos que presentaban células EV como los que no). Serían necesarios más estudios que comparasen lesiones asociadas a inmunodepresión con otras que no. Es posible que estas células EV sean el resultado de los efectos citopáticos de determinados subtipos de VPH, como el VPH-5 o el VPH-8, aunque sería necesario demostrar esta hipótesis mediante técnicas de PCR o similar.

Palabras clave:
epidermodisplasia verruciforme
inmunodepresión
papilomavirus
elastosis solar
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2006 August C. Stiefel Award.

Copyright © 2008. Academia Española de Dermatología y Venereología and Elsevier España, S.L.
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