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Vol. 99. Núm. 7.
Páginas 528-531 (septiembre 2008)
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Vol. 99. Núm. 7.
Páginas 528-531 (septiembre 2008)
Advances in dermatology
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Clinical Applications of Reflectance Confocal Microscopy in the Management of Cutaneous Tumors
Aplicaciones Clínicas de la Microscopía Confocal de Reflectancia en el Manejo de Los Tumores Cutáneos
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S. González
Autor para correspondencia
gonzals6@mskcc.org

Correspondence: Dermatology Service, Memorial Sloan-Kettering Cancer Center, 160 E 53rd Street, New York, NY10022, USA.
Dermatology Service. Memorial Sloan-Kettering Cancer Center, New York, USA Servicio de Dermatología. Hospital Ramón y Cajal, Madrid, Spain
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Abstract

Reflectance confocal microscopy is a noninvasive tool that allows skin cells and structure to be imaged in real time. The technique has been used to assess benign and malignant lesions and has shown great potential in basic research and clinical dermatology. As might be expected, it also has great potential in longitudinal clinical studies and in the evaluation of dynamic processes such as those that occur after exposure to UV radiation or during the tumor response to noninvasive therapy. This article briefly describes the fundamental aspects and basic principles of reflectance confocal microscopy and discusses its clinical applications essentially in the management of cutaneous tumors. We also consider the limitations of the technique associated with the optical properties of the skin, with instrumentation, and with interpretation of the images.

Key words:
reflectance confocal microscopy
cutaneous tumors
virtual biopsy
noninvasive technology
Resumen

La microscopía confocal de reflectancia es una herramienta de imágenes que permite la visualización de células y estructuras de la piel de forma no invasiva y en tiempo real. Ésta se ha usado para la evaluación de lesiones benignas y malignas, demostrando un gran potencial para aplicaciones en investigación básica y en dermatología clínica. Como cabría pensar, esta tecnología tiene un gran potencial en estudios clínicos longitudinales y en la evaluación de procesos dinámicos como los que acontecen tras la exposición a radiación ultravioleta o durante la respuesta de los tumores a terapias no invasivas. Este artículo discute brevemente los fundamentos o principios básicos de la microscopía confocal de reflectancia, sus aplicaciones clínicas, fundamentalmente en el manejo de los tumores cutáneos, y las limitaciones que la técnica presenta asociadas a las propiedades ópticas de la piel, con la instrumentación y con la interpretación de las imágenes.

Palabras clave:
microscopía confocal de reflectancia
tumores cutáneos
biopsia virtual
tecnología no invasiva
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References
[1.]
N.D. Gladkova, G.A. Petrova, N.K. Nikulin, S.G. Radenska-Lopovok, L.B. Snopova, Y.P. Chumakov, et al.
In vivo optical coherence tomography imaging of human skin: norm and pathology.
Skin Res Technol, 6 (2000), pp. 6-16
[2.]
M.C. Pierce, J. Strasswimmer, B.H. Park, B. Cense, J.F. de Boer.
Advances in optical coherence tomography imaging for dermatology.
J Invest Dermatol, 123 (2004), pp. 458-463
[3.]
B.D. Fornage.
High-frequency sonography of the skin.
Eur J Ultrasound, 2 (1995), pp. 173-182
[4.]
B. Bessoud, N. Lassau, S. Koscielny, C. Longvert, M.-F. Avril, P. Duvillard, et al.
High-frequency sonography and color Doppler in the management of pigmented skin lesions.
Ultrasound Med Biol, 29 (2003), pp. 875-879
[5.]
M. Rajadhyaksha, M. Grossman, D. Esterowitz, R.H. Webb, R.R. Anderson.
In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast.
J Invest Dermatol, 104 (1995), pp. 946-952
[6.]
M. Rajadhyaksha, S. González, J.M. Zavislan, R.R. Anderson, R.H. Webb.
In vivo confocal scanning laser microscopy of human skin II: advances in instrumentation and comparison with histology.
J Invest Dermatol, 113 (1999), pp. 293-303
[7.]
S. González, K. Swindells, M. Rajadhyaksha, A. Torres.
Changing paradigms in dermatology: confocal microscopy in clinical and surgical dermatology.
Clin Dermatol, 21 (2003), pp. 359-369
[8.]
Reflectance confocal microscopy of cutaneous tumors: an atlas with clinical, dermoscopic and histological correlations,
[9.]
M. Rajadhyaksha.
Confocal reflectance microscopy: diagnosis of skin cancer without biopsy? Frontiers of Engineering.
National Academies Press, (1999),
[10.]
S. González, R. Sackstein, R.R. Anderson, M. Rajadhyaksha.
Real-time evidence of in vivo leukocyte trafficking in human skin by reflectance confocal microscopy.
J Invest Dermatol, 117 (2001), pp. 384-386
[11.]
T. Yamashita, T. Kuwahara, S. González, M. Takahashi.
Noninvasive visualization of melanin and melanocytes by reflectance-mode confocal microscopy.
J Invest Dermatol, 124 (2005), pp. 235-240
[12.]
C. Curiel-Lewandrowski, C.M. Williams, K.J. Swindells, S.R. Tahan, S. Astner, R.A. Frankenthaler, et al.
Use of in vivo confocal microscopy in malignant melanoma: an aid in diagnosis and assessment of surgical and nonsurgical therapeutic approaches.
Arch Dermatol, 140 (2004), pp. 1127-1132
[13.]
M. Huzaira, F. Rius, M. Rajadhyaksha, R.R. Anderson, S. González.
Topographic variations in normal skin, as viewed by in vivo reflectance confocal microscopy.
J Invest Dermatol, 116 (2001), pp. 846-852
[14.]
M. Rajadhyaksha, G. Menaker, T. Flotte, P.J. Dwyer, S. González.
Confocal examination of nonmelanoma cancers in thick skin excisions to potentially guide Mohs micrographic surgery without frozen histopathology.
J Invest Dermatol, 117 (2001), pp. 1137-1143
[15.]
A.L. Agero, M. Gill, M. Ardigo, P. Myskowski, A.C. Halpern, S. González.
In vivo reflectance confocal microscopy of mycosis fungoides: A preliminary study.
J Am Acad Dermatol, 57 (2007), pp. 435-441
[16.]
C.S. Chen, M. Elias, K. Busam, M. Rajadhyaksha, A.A. Marghoob.
Multimodal in vivo optical imaging, including confocal microscopy, facilitates presurgical margin mapping for clinically complex lentigo maligna melanoma.
Br J Dermatol, 153 (2005), pp. 1031-1036
[17.]
Z.S. Tannous, M.C. Mihm, T.J. Flotte, S. González.
In vivo examination of lentigo maligna and malignant melanoma in situ, lentigo maligna type by near-infrared reflectance confocal microscopy: comparison of in vivo confocal images with histologic sections.
J Am Acad Dermatol, 46 (2002), pp. 260-263
[18.]
G. Pellacani, A.M. Cesinaro, S. Seidenari.
In vivo assessment of melanocytic nests in nevi and melanomas by reflectance confocal microscopy.
Mod Pathol, 18 (2005), pp. 469-474
[19.]
K.J. Busam, K. Hester, C. Charles, D.L. Sachs, C.R. Antonescu, S. González, et al.
Detection of clinically amelanotic malignant melanoma and assessment of its margins by in vivo confocal scanning laser microscopy.
Arch Dermatol, 137 (2001), pp. 923-929
[20.]
M. Trehan, K. Swindells, C.R. Taylor, A.L. Racette, S. González.
Confocal microscopy imaging of actinic keratoses postphotodynamic therapy with 5-ALA.
Paper presented at: 20th World Congress of Dermatology,
[21.]
A. Torres, A. Niemeyer, B. Berkes, D. Marra, C. Schanbacher, S. González, et al.
5% imiquimod cream and reflectancemode confocal microscopy as adjunct modalities to Mohs micrographic surgery for treatment of basal cell carcinoma.
Dermatol Surg, 30 (2004), pp. 1462-1469
[22.]
M. Goldgeier, C.A. Fox, J.M. Zavislan, D. Harris, S. González.
Noninvasive imaging, treatment, and microscopic confirmation of clearance of basal cell carcinoma.
Dermatol Surg, 29 (2003), pp. 205-210
[23.]
D.E. Marra, A. Torres, C.F. Schanbacher, S. González.
Detection of residual basal cell carcinoma by in vivo confocal microscopy.
Dermatol Surg, 31 (2005), pp. 538-541
Copyright © 2008. Academia Española de Dermatología y Venereología and Elsevier España, S.L.
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