Article information
Abstract
The European Biomedicine and Health (BIOMED-2) Concerted Action Project BMH4-CT98-3936 has defined standardized protocols for polymerase chain reaction (PCR) amplification of different loci of the T-cell receptor (TCR) and immunoglobulin (Ig) genes with a view to achieving greater sensitivity and specificity in the assessment of clonality of lymphoid neoplasms. To assess T-cell clonality, analysis of TCRβ gene and TCRδ rearrangements (useful in cases of Tγδ+ cell neoplasms) is proposed alongside that of TCRγ. For analysis of B-cell clonality, along with the framework (FR) III segment of the IgH gene, other segments are studied (FRI, FRII) in addition to Igγ and Igκ genes or incomplete DJ rearrangements of the IgH gene and the κ deleting element. The results of the amplification are read using automatic reading systems (GeneScan) or using a heteroduplex system.
Key words:
cutaneous lymphoma
gene rearrangement
clonality
polymerase chain reaction
BIOMED-2
GeneScan
Resumen
El proyecto europeo BIOMED-2 Concerted Action BMH4-CT98-3936 describe protocolos estandarizados de amplificación por reacción en cadena de la polimerasa (PCR) de los diferentes loci de los genes del receptor de la célula T (TCR) e inmunoglobulinas (Ig) con el objetivo de obtener una mayor sensibilidad y especificidad en el estudio de clonalidad de las neoplasias linfoides. En el estudio de clonalidad T, además del TCRγ se propone el estudio adicional de los reordenamientos del gen TCRβ y del locus TCRδ (útil en casos de neoplasias de células Tγδ+). En el estudio de clonalidad B, junto al segmento FRIII del gen IgH se diseña el estudio de otros segmentos (FRI, FRII), así como de los genes Igλ e Igκ o reordenamientos incompletos DJ del gen IgH y κde (kappa deleting element). La lectura de los resultados de la amplificación se realiza mediante sistemas automatizados de lectura (GeneScan) o mediante el sistema heterodúplex.
Palabras clave:
linfoma cutáneo
reordenamiento genético
clonalidad
reacción en cadena de la polimerasa
BIOMED-2
GeneScan
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This study was partly funded by the FIS 03/394 grant from the Spanish Ministry of Health and Consumer Affairs.
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