array:24 [
  "pii" => "S0001731024003430"
  "issn" => "00017310"
  "doi" => "10.1016/j.ad.2024.04.003"
  "estado" => "S300"
  "fechaPublicacion" => "2024-06-01"
  "aid" => "3937"
  "copyright" => "AEDV"
  "copyrightAnyo" => "2022"
  "documento" => "simple-article"
  "crossmark" => 1
  "subdocumento" => "crp"
  "cita" => "Actas Dermosifiliogr. 2024;115:T597-T598"
  "abierto" => array:3 [
    "ES" => true
    "ES2" => true
    "LATM" => true
  ]
  "gratuito" => true
  "lecturas" => array:1 [
    "total" => 0
  ]
  "itemSiguiente" => array:18 [
    "pii" => "S0001731023008219"
    "issn" => "00017310"
    "doi" => "10.1016/j.ad.2023.08.012"
    "estado" => "S300"
    "fechaPublicacion" => "2024-06-01"
    "aid" => "3693"
    "copyright" => "AEDV"
    "documento" => "simple-article"
    "crossmark" => 1
    "subdocumento" => "crp"
    "cita" => "Actas Dermosifiliogr. 2024;115:599-600"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:1 [
      "total" => 0
    ]
    "es" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Casos para el diagn&#243;stico</span>"
      "titulo" => "Enfermedad ampollosa posterior a la vacunaci&#243;n frente al SARS-CoV-2"
      "tienePdf" => "es"
      "tieneTextoCompleto" => "es"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "599"
          "paginaFinal" => "600"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "en" => array:1 [
          "titulo" => "Blistering Disease After COVID-19 Vaccination"
        ]
      ]
      "contieneTextoCompleto" => array:1 [
        "es" => true
      ]
      "contienePdf" => array:1 [
        "es" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:7 [
          "identificador" => "fig0005"
          "etiqueta" => "Figura 1"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr1.jpeg"
              "Alto" => 1340
              "Ancho" => 1340
              "Tamanyo" => 466429
            ]
          ]
          "descripcion" => array:1 [
            "es" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Fotograf&#237;as cl&#237;nicas&#46; A y B&#41; Muslos con ampollas tensas y costras hemorr&#225;gicas distribuidas en rosetas&#46; C&#41; M&#225;culas hipopigmentadas residuales&#44; con un patr&#243;n en rosetas&#46;</p>"
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "L&#46;M&#46; Pulgar&#237;n, K&#46; Mu&#241;et&#243;n-Ram&#237;rez, R&#46;A&#46; Rueda"
          "autores" => array:3 [
            0 => array:2 [
              "nombre" => "L&#46;M&#46;"
              "apellidos" => "Pulgar&#237;n"
            ]
            1 => array:2 [
              "nombre" => "K&#46;"
              "apellidos" => "Mu&#241;et&#243;n-Ram&#237;rez"
            ]
            2 => array:2 [
              "nombre" => "R&#46;A&#46;"
              "apellidos" => "Rueda"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "es"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0001731023008219?idApp=UINPBA000044"
    "url" => "/00017310/0000011500000006/v1_202406021150/S0001731023008219/v1_202406021150/es/main.assets"
  ]
  "itemAnterior" => array:18 [
    "pii" => "S0001731022010468"
    "issn" => "00017310"
    "doi" => "10.1016/j.ad.2022.09.022"
    "estado" => "S300"
    "fechaPublicacion" => "2024-06-01"
    "aid" => "3333"
    "copyright" => "AEDV"
    "documento" => "simple-article"
    "crossmark" => 1
    "subdocumento" => "crp"
    "cita" => "Actas Dermosifiliogr. 2024;115:597-8"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:1 [
      "total" => 0
    ]
    "es" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Casos para el diagn&#243;stico</span>"
      "titulo" => "Lesi&#243;n de la mucosa oral negativa para la prote&#237;na S100"
      "tienePdf" => "es"
      "tieneTextoCompleto" => "es"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "597"
          "paginaFinal" => "598"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "en" => array:1 [
          "titulo" => "S100-Protein&#8211;Negative Mouth Lesion"
        ]
      ]
      "contieneTextoCompleto" => array:1 [
        "es" => true
      ]
      "contienePdf" => array:1 [
        "es" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:6 [
          "identificador" => "fig0005"
          "etiqueta" => "Figura 1"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr1.jpeg"
              "Alto" => 801
              "Ancho" => 1067
              "Tamanyo" => 284268
            ]
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "K&#46; Saez de Gordoa, L&#46; Alos, R&#46; Albero-Gonz&#225;lez"
          "autores" => array:3 [
            0 => array:2 [
              "nombre" => "K&#46;"
              "apellidos" => "Saez de Gordoa"
            ]
            1 => array:2 [
              "nombre" => "L&#46;"
              "apellidos" => "Alos"
            ]
            2 => array:2 [
              "nombre" => "R&#46;"
              "apellidos" => "Albero-Gonz&#225;lez"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "es"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0001731022010468?idApp=UINPBA000044"
    "url" => "/00017310/0000011500000006/v1_202406021150/S0001731022010468/v1_202406021150/es/main.assets"
  ]
  "asociados" => array:1 [
    0 => array:18 [
      "pii" => "S0001731022010468"
      "issn" => "00017310"
      "doi" => "10.1016/j.ad.2022.09.022"
      "estado" => "S300"
      "fechaPublicacion" => "2024-06-01"
      "aid" => "3333"
      "copyright" => "AEDV"
      "documento" => "simple-article"
      "crossmark" => 1
      "subdocumento" => "crp"
      "cita" => "Actas Dermosifiliogr. 2024;115:597-8"
      "abierto" => array:3 [
        "ES" => true
        "ES2" => true
        "LATM" => true
      ]
      "gratuito" => true
      "lecturas" => array:1 [
        "total" => 0
      ]
      "es" => array:11 [
        "idiomaDefecto" => true
        "cabecera" => "<span class="elsevierStyleTextfn">Casos para el diagn&#243;stico</span>"
        "titulo" => "Lesi&#243;n de la mucosa oral negativa para la prote&#237;na S100"
        "tienePdf" => "es"
        "tieneTextoCompleto" => "es"
        "paginas" => array:1 [
          0 => array:2 [
            "paginaInicial" => "597"
            "paginaFinal" => "598"
          ]
        ]
        "titulosAlternativos" => array:1 [
          "en" => array:1 [
            "titulo" => "S100-Protein&#8211;Negative Mouth Lesion"
          ]
        ]
        "contieneTextoCompleto" => array:1 [
          "es" => true
        ]
        "contienePdf" => array:1 [
          "es" => true
        ]
        "resumenGrafico" => array:2 [
          "original" => 0
          "multimedia" => array:6 [
            "identificador" => "fig0005"
            "etiqueta" => "Figura 1"
            "tipo" => "MULTIMEDIAFIGURA"
            "mostrarFloat" => true
            "mostrarDisplay" => false
            "figura" => array:1 [
              0 => array:4 [
                "imagen" => "gr1.jpeg"
                "Alto" => 801
                "Ancho" => 1067
                "Tamanyo" => 284268
              ]
            ]
          ]
        ]
        "autores" => array:1 [
          0 => array:2 [
            "autoresLista" => "K&#46; Saez de Gordoa, L&#46; Alos, R&#46; Albero-Gonz&#225;lez"
            "autores" => array:3 [
              0 => array:2 [
                "nombre" => "K&#46;"
                "apellidos" => "Saez de Gordoa"
              ]
              1 => array:2 [
                "nombre" => "L&#46;"
                "apellidos" => "Alos"
              ]
              2 => array:2 [
                "nombre" => "R&#46;"
                "apellidos" => "Albero-Gonz&#225;lez"
              ]
            ]
          ]
        ]
      ]
      "idiomaDefecto" => "es"
      "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0001731022010468?idApp=UINPBA000044"
      "url" => "/00017310/0000011500000006/v1_202406021150/S0001731022010468/v1_202406021150/es/main.assets"
    ]
  ]
  "en" => array:15 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Case for Diagnosis</span>"
    "titulo" => " S100-protein&#8211;negative Mouth Lesion"
    "tieneTextoCompleto" => true
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "T597"
        "paginaFinal" => "T598"
      ]
    ]
    "autores" => array:1 [
      0 => array:4 [
        "autoresLista" => "K&#46; Saez de Gordoa, L&#46; Alos, R&#46; Albero-Gonz&#225;lez"
        "autores" => array:3 [
          0 => array:4 [
            "nombre" => "K&#46;"
            "apellidos" => "Saez de Gordoa"
            "email" => array:1 [
              0 => "saezdegord@clinic.cat"
            ]
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "cor0005"
              ]
            ]
          ]
          1 => array:2 [
            "nombre" => "L&#46;"
            "apellidos" => "Alos"
          ]
          2 => array:2 [
            "nombre" => "R&#46;"
            "apellidos" => "Albero-Gonz&#225;lez"
          ]
        ]
        "afiliaciones" => array:1 [
          0 => array:2 [
            "entidad" => "Servicio de Anatom&#237;a Patol&#243;gica&#44; CDB&#44; Hospital Cl&#237;nic&#44; Barcelona&#44; Espa&#241;a"
            "identificador" => "aff0005"
          ]
        ]
        "correspondencia" => array:1 [
          0 => array:3 [
            "identificador" => "cor0005"
            "etiqueta" => "&#8270;"
            "correspondencia" => "Corresponding author&#46;"
          ]
        ]
      ]
    ]
    "titulosAlternativos" => array:1 [
      "es" => array:1 [
        "titulo" => "Lesi&#243;n de la mucosa oral negativa para la prote&#237;na S100"
      ]
    ]
    "resumenGrafico" => array:2 [
      "original" => 0
      "multimedia" => array:6 [
        "identificador" => "fig0005"
        "etiqueta" => "Figure 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 602
            "Ancho" => 802
            "Tamanyo" => 159831
          ]
        ]
      ]
    ]
    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0005">Medical history</span><p id="par0005" class="elsevierStylePara elsevierViewall">An 83-year-old woman with a past medical history of atrial fibrillation and hypothyroidism presented with a 1-year history painful and bleeding lesion of the hard palate&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Physical examination</span><p id="par0010" class="elsevierStylePara elsevierViewall">A 5cm nodular reddish lesion was found on the hard palate&#44; with some surrounding pigmentation &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>A&#41;&#46; No locoregional lymphadenopathies were described&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Histopathology</span><p id="par0015" class="elsevierStylePara elsevierViewall">Histopathological examination revealed the presence of a non-pigmented&#44; submucosal cellular proliferation of atypical characteristics consisting of large&#44; discohesive&#44; epithelioid and spindle cells&#44; with pleomorphic nuclei&#44; visible nucleoli&#44; and multiple mitotic figures &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>B-D&#41;&#46; Due to the absence of a specific morphological pattern&#44; a broad differential diagnosis was considered&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Other supplementary tests</span><p id="par0020" class="elsevierStylePara elsevierViewall">The initial immunohistochemical study performed included epithelial &#40;broad-spectrum keratins&#58; CK AE1&#47;AE3&#41;&#44; hematological &#40;CD20 and CD68&#41;&#44; muscular &#40;&#945;-actin&#41;&#44; and melanocytic markers &#40;S100 protein&#41;&#46; All tested negative&#44; with appropriate positive external and&#47;or internal controls &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>A and B&#41;&#46; A second immunohistochemical panel conducted included EMA&#44; p40&#44; CK19&#44; CD45&#44; desmin&#44; ERG&#44; and SOX10&#46; Tumor cells exhibited intense expression of SOX10 &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>C&#41;&#44; which prompted further study&#44; and eventually revealed the diffuse expression of PRAME and HMB45&#44; and focal positivity for Melan-A &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>D-F&#41;&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia><p id="par0025" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">What is your diagnosis&#63;</span></p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Diagnosis</span><p id="par0030" class="elsevierStylePara elsevierViewall">The definitive histopathological diagnosis was invasive melanoma of the oral mucosa&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Course of the disease and treatment</span><p id="par0035" class="elsevierStylePara elsevierViewall">The molecular analysis performed using the NGS Oncomine<span class="elsevierStyleSup">TM</span> Focus panel &#40;Thermo Fisher Scientific&#41; detected gains in the KIT &#40;4q12&#41; and CDK4 &#40;12q14&#46;1&#41; genes&#44; without any mutations or fusions being reported in the studied DNA and RNA regions&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">A PET-CT scan performed during the staging process revealed the presence of bilateral lung nodules with metabolic activity&#46; The patient did not start cancer treatment due to rapid disease progression and died 2 months after diagnosis&#46;</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Discussion</span><p id="par0045" class="elsevierStylePara elsevierViewall">Oral mucosal melanoma &#40;OMM&#41; is a rare neoplasm&#44; representing between 0&#46;2&#37; and 0&#46;5&#37; of all oral neoplasms and 1&#37; of melanomas&#46;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">1</span></a> The most commonly affected locations are the hard palate and the maxillary and mandibular gingiva&#46;<a class="elsevierStyleCrossRefs" href="#bib0040"><span class="elsevierStyleSup">2&#44;3</span></a> Compared to its cutaneous counterpart&#44; OMM occurs in older patients &#40;around the 5<span class="elsevierStyleSup">th</span> decade of life&#41;&#44; and is more common in white individuals than in darker skin types&#44; although with a lower predisposition than cutaneous melanoma&#46;<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">2</span></a></p><p id="par0050" class="elsevierStylePara elsevierViewall">The most common clinical presentation is an asymptomatic flat or nodular lesion that may have satellite lesions too&#46;<a class="elsevierStyleCrossRefs" href="#bib0035"><span class="elsevierStyleSup">1&#44;3</span></a> OMMs are often pigmented whether uniformly or heterogeneously&#46; Nonetheless&#44; they can also exhibit an amelanotic lesion in 10&#37; to 30&#37; of the patients&#46; Ulceration and bleeding may occur&#44; especially in later stages of the disease&#46;<a class="elsevierStyleCrossRefs" href="#bib0035"><span class="elsevierStyleSup">1&#44;2</span></a></p><p id="par0055" class="elsevierStylePara elsevierViewall">A recent literature search revealed 2 case series&#44; which reported on 1 case of OMM with negative immunohistochemistry for the S100 protein in each of them&#46;<a class="elsevierStyleCrossRefs" href="#bib0050"><span class="elsevierStyleSup">4&#44;5</span></a> Prasad et al&#46; reported 1 negative case of a series of 35 patients&#46;<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">4</span></a> The tumor tested positive for the remaining markers &#40;T311&#44; A103&#44; HMB45&#44; and D5&#41;&#44; with sensitivity rates of 94&#37;&#44; 85&#37;&#44; 71&#37;&#44; and 74&#37;&#44; respectively&#46; Yu et al&#46; reported a series of 6 cases of OMM with a tumor that tested negative for the S100 protein and Melan-A&#44; and positive for HMB4<span class="elsevierStyleSup">5</span>&#46; In this study&#44; the sensitivity rates of HMB45<span class="elsevierStyleSup">5</span> and Melan-A were 100&#37; and 67&#37;&#44; respectively&#46;</p><p id="par0060" class="elsevierStylePara elsevierViewall">For the molecular profile of OMM&#44; changes in copy number and amplifications are a common finding&#44; including amplifications of 4q12 &#40;KIT&#41; and 12q14 &#40;CDK4&#41; as seen in this case&#46;<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">6</span></a></p><p id="par0065" class="elsevierStylePara elsevierViewall">In conclusion&#44; the expression of the S100 protein for the diagnosis of OMM shows high sensitivity though it can be negative in up to 3&#37; of the cases&#46; Therefore&#44; in the initial study of an undifferentiated malignant tumor in this region&#44; using multiple melanocytic markers is recommended to safely exclude the diagnosis of melanoma&#46;</p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Conflicts of interest</span><p id="par0070" class="elsevierStylePara elsevierViewall">None declared&#46;</p></span></span>"
    "textoCompletoSecciones" => array:1 [
      "secciones" => array:10 [
        0 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Medical history"
        ]
        1 => array:2 [
          "identificador" => "sec0010"
          "titulo" => "Physical examination"
        ]
        2 => array:2 [
          "identificador" => "sec0015"
          "titulo" => "Histopathology"
        ]
        3 => array:2 [
          "identificador" => "sec0020"
          "titulo" => "Other supplementary tests"
        ]
        4 => array:2 [
          "identificador" => "sec0025"
          "titulo" => "Diagnosis"
        ]
        5 => array:2 [
          "identificador" => "sec0030"
          "titulo" => "Course of the disease and treatment"
        ]
        6 => array:2 [
          "identificador" => "sec0035"
          "titulo" => "Discussion"
        ]
        7 => array:2 [
          "identificador" => "sec0040"
          "titulo" => "Conflicts of interest"
        ]
        8 => array:2 [
          "identificador" => "xack748922"
          "titulo" => "Acknowledgements"
        ]
        9 => array:1 [
          "titulo" => "References"
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "multimedia" => array:2 [
      0 => array:6 [
        "identificador" => "fig0005"
        "etiqueta" => "Figure 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 602
            "Ancho" => 802
            "Tamanyo" => 159831
          ]
        ]
      ]
      1 => array:6 [
        "identificador" => "fig0010"
        "etiqueta" => "Figure 2"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr2.jpeg"
            "Alto" => 606
            "Ancho" => 806
            "Tamanyo" => 171991
          ]
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0015"
          "bibliografiaReferencia" => array:6 [
            0 => array:3 [
              "identificador" => "bib0035"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Oral malignant melanoma&#58; a review of the literature"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "M&#46; Meleti"
                            1 => "C&#46;R&#46; Leemans"
                            2 => "W&#46;J&#46; Mooi"
                            3 => "P&#46; Vescovi"
                            4 => "I&#46; van der Waal"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.oraloncology.2006.04.001"
                      "Revista" => array:6 [
                        "tituloSerie" => "Oral Oncol&#46;"
                        "fecha" => "2007 Feb"
                        "volumen" => "43"
                        "paginaInicial" => "116"
                        "paginaFinal" => "121"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16931116"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0040"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Premalignant and malignant oral mucosal lesions&#58; Clinical and pathological findings"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "M&#46;B&#46;C&#46; Maymone"
                            1 => "R&#46;O&#46; Greer"
                            2 => "J&#46; Kesecker"
                            3 => "P&#46;C&#46; Sahitya"
                            4 => "L&#46;K&#46; Burdine"
                            5 => "A&#46;D&#46; Cheng"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.jaad.2018.09.060"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Am Acad Dermatol&#46;"
                        "fecha" => "2019 Jul"
                        "volumen" => "81"
                        "paginaInicial" => "59"
                        "paginaFinal" => "71"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/30447325"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0045"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Mucosal melanoma of the head and neck"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "P&#46;A&#46; Ascierto"
                            1 => "R&#46; Accorona"
                            2 => "G&#46; Botti"
                            3 => "D&#46; Farina"
                            4 => "P&#46; Fossati"
                            5 => "G&#46; Gatta"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.critrevonc.2017.01.019:136-52"
                      "Revista" => array:6 [
                        "tituloSerie" => "Crit Rev Oncol Hematol&#46;"
                        "fecha" => "2017 Apr"
                        "volumen" => "112"
                        "paginaInicial" => "136"
                        "paginaFinal" => "152"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/28325255"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib0050"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Expression of melanocytic differentiation markers in malignant melanomas of the oral and sinonasal mucosa"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "M&#46;L&#46; Prasad"
                            1 => "A&#46;A&#46; Jungbluth"
                            2 => "K&#46; Iversen"
                            3 => "A&#46;G&#46; Huvos"
                            4 => "K&#46;J&#46; Busam"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1097/00000478-200106000-00010"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Surg Pathol&#46;"
                        "fecha" => "2001 Jun"
                        "volumen" => "25"
                        "paginaInicial" => "782"
                        "paginaFinal" => "787"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11395556"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0055"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "HMB-45 may be a more sensitive maker than S-100 or Melan-A for immunohistochemical diagnosis of primary oral and nasal mucosal melanomas"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "C&#46;H&#46; Yu"
                            1 => "H&#46;H&#46; Chen"
                            2 => "C&#46;M&#46; Liu"
                            3 => "Y&#46;M&#46; Jeng"
                            4 => "J&#46;T&#46; Wang"
                            5 => "Y&#46;P&#46; Wang"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/j.1600-0714.2005.00340.x"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Oral Pathol Med&#46;"
                        "fecha" => "2005 Oct"
                        "volumen" => "34"
                        "paginaInicial" => "540"
                        "paginaFinal" => "545"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16138892"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0060"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Distinct sets of genetic alterations in melanoma"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "J&#46;A&#46; Curtin"
                            1 => "J&#46; Fridlyand"
                            2 => "T&#46; Kageshita"
                            3 => "H&#46;N&#46; Patel"
                            4 => "K&#46;J&#46; Busam"
                            5 => "H&#46; Kutzner"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1056/NEJMoa050092"
                      "Revista" => array:7 [
                        "tituloSerie" => "N Engl J Med&#46;"
                        "fecha" => "2005 Nov 17"
                        "volumen" => "353"
                        "paginaInicial" => "2135"
                        "paginaFinal" => "2147"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16291983"
                            "web" => "Medline"
                          ]
                        ]
                        "itemHostRev" => array:3 [
                          "pii" => "S1473309922008167"
                          "estado" => "S300"
                          "issn" => "14733099"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
    "agradecimientos" => array:1 [
      0 => array:4 [
        "identificador" => "xack748922"
        "titulo" => "Acknowledgements"
        "texto" => "<p id="par0075" class="elsevierStylePara elsevierViewall">We wish to thank Dr&#46; Pedro Jares for his collaboration in the molecular study of the case and Dr&#46; Carles Mart&#237; for providing relevant clinical data&#46;</p>"
        "vista" => "all"
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/00017310/0000011500000006/v1_202406021150/S0001731024003430/v1_202406021150/en/main.assets"
  "Apartado" => array:4 [
    "identificador" => "6161"
    "tipo" => "SECCION"
    "es" => array:2 [
      "titulo" => "Casos para el diagn&#243;stico"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "es"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/00017310/0000011500000006/v1_202406021150/S0001731024003430/v1_202406021150/en/main.pdf?idApp=UINPBA000044&text.app=https://actasdermo.org/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0001731024003430?idApp=UINPBA000044"
]
Compartir
Información de la revista
Vol. 115. Núm. 6.
Páginas T597-T598 (junio 2024)
Compartir
Compartir
Descargar PDF
Más opciones de artículo
Visitas
1612
Vol. 115. Núm. 6.
Páginas T597-T598 (junio 2024)
Case for Diagnosis
Acceso a texto completo
S100-protein–negative Mouth Lesion
Lesión de la mucosa oral negativa para la proteína S100
Visitas
1612
K. Saez de Gordoa
Autor para correspondencia
saezdegord@clinic.cat

Corresponding author.
, L. Alos, R. Albero-González
Servicio de Anatomía Patológica, CDB, Hospital Clínic, Barcelona, España
Contenido relacionado
Actas Dermosifiliogr. 2024;115:597-810.1016/j.ad.2022.09.022
K. Saez de Gordoa, L. Alos, R. Albero-González
Este artículo ha recibido
Información del artículo
Texto completo
Bibliografía
Descargar PDF
Estadísticas
Figuras (2)
Texto completo
Medical history

An 83-year-old woman with a past medical history of atrial fibrillation and hypothyroidism presented with a 1-year history painful and bleeding lesion of the hard palate.

Physical examination

A 5cm nodular reddish lesion was found on the hard palate, with some surrounding pigmentation (Fig. 1A). No locoregional lymphadenopathies were described.

Figure 1
(0.15MB).
Histopathology

Histopathological examination revealed the presence of a non-pigmented, submucosal cellular proliferation of atypical characteristics consisting of large, discohesive, epithelioid and spindle cells, with pleomorphic nuclei, visible nucleoli, and multiple mitotic figures (Fig. 1B-D). Due to the absence of a specific morphological pattern, a broad differential diagnosis was considered.

Other supplementary tests

The initial immunohistochemical study performed included epithelial (broad-spectrum keratins: CK AE1/AE3), hematological (CD20 and CD68), muscular (α-actin), and melanocytic markers (S100 protein). All tested negative, with appropriate positive external and/or internal controls (Fig. 2A and B). A second immunohistochemical panel conducted included EMA, p40, CK19, CD45, desmin, ERG, and SOX10. Tumor cells exhibited intense expression of SOX10 (Fig. 2C), which prompted further study, and eventually revealed the diffuse expression of PRAME and HMB45, and focal positivity for Melan-A (Fig. 2D-F).

Figure 2
(0.16MB).

What is your diagnosis?

Diagnosis

The definitive histopathological diagnosis was invasive melanoma of the oral mucosa.

Course of the disease and treatment

The molecular analysis performed using the NGS OncomineTM Focus panel (Thermo Fisher Scientific) detected gains in the KIT (4q12) and CDK4 (12q14.1) genes, without any mutations or fusions being reported in the studied DNA and RNA regions.

A PET-CT scan performed during the staging process revealed the presence of bilateral lung nodules with metabolic activity. The patient did not start cancer treatment due to rapid disease progression and died 2 months after diagnosis.

Discussion

Oral mucosal melanoma (OMM) is a rare neoplasm, representing between 0.2% and 0.5% of all oral neoplasms and 1% of melanomas.1 The most commonly affected locations are the hard palate and the maxillary and mandibular gingiva.2,3 Compared to its cutaneous counterpart, OMM occurs in older patients (around the 5th decade of life), and is more common in white individuals than in darker skin types, although with a lower predisposition than cutaneous melanoma.2

The most common clinical presentation is an asymptomatic flat or nodular lesion that may have satellite lesions too.1,3 OMMs are often pigmented whether uniformly or heterogeneously. Nonetheless, they can also exhibit an amelanotic lesion in 10% to 30% of the patients. Ulceration and bleeding may occur, especially in later stages of the disease.1,2

A recent literature search revealed 2 case series, which reported on 1 case of OMM with negative immunohistochemistry for the S100 protein in each of them.4,5 Prasad et al. reported 1 negative case of a series of 35 patients.4 The tumor tested positive for the remaining markers (T311, A103, HMB45, and D5), with sensitivity rates of 94%, 85%, 71%, and 74%, respectively. Yu et al. reported a series of 6 cases of OMM with a tumor that tested negative for the S100 protein and Melan-A, and positive for HMB45. In this study, the sensitivity rates of HMB455 and Melan-A were 100% and 67%, respectively.

For the molecular profile of OMM, changes in copy number and amplifications are a common finding, including amplifications of 4q12 (KIT) and 12q14 (CDK4) as seen in this case.6

In conclusion, the expression of the S100 protein for the diagnosis of OMM shows high sensitivity though it can be negative in up to 3% of the cases. Therefore, in the initial study of an undifferentiated malignant tumor in this region, using multiple melanocytic markers is recommended to safely exclude the diagnosis of melanoma.

Conflicts of interest

None declared.

Acknowledgements

We wish to thank Dr. Pedro Jares for his collaboration in the molecular study of the case and Dr. Carles Martí for providing relevant clinical data.

References
[1]
M. Meleti, C.R. Leemans, W.J. Mooi, P. Vescovi, I. van der Waal.
Oral malignant melanoma: a review of the literature.
Oral Oncol., 43 (2007 Feb), pp. 116-121
[2]
M.B.C. Maymone, R.O. Greer, J. Kesecker, P.C. Sahitya, L.K. Burdine, A.D. Cheng, et al.
Premalignant and malignant oral mucosal lesions: Clinical and pathological findings.
J Am Acad Dermatol., 81 (2019 Jul), pp. 59-71
[3]
P.A. Ascierto, R. Accorona, G. Botti, D. Farina, P. Fossati, G. Gatta, et al.
Mucosal melanoma of the head and neck.
Crit Rev Oncol Hematol., 112 (2017 Apr), pp. 136-152
[4]
M.L. Prasad, A.A. Jungbluth, K. Iversen, A.G. Huvos, K.J. Busam.
Expression of melanocytic differentiation markers in malignant melanomas of the oral and sinonasal mucosa.
Am J Surg Pathol., 25 (2001 Jun), pp. 782-787
[5]
C.H. Yu, H.H. Chen, C.M. Liu, Y.M. Jeng, J.T. Wang, Y.P. Wang, et al.
HMB-45 may be a more sensitive maker than S-100 or Melan-A for immunohistochemical diagnosis of primary oral and nasal mucosal melanomas.
J Oral Pathol Med., 34 (2005 Oct), pp. 540-545
[6]
J.A. Curtin, J. Fridlyand, T. Kageshita, H.N. Patel, K.J. Busam, H. Kutzner, et al.
Distinct sets of genetic alterations in melanoma.
N Engl J Med., 353 (2005 Nov 17), pp. 2135-2147
Copyright © 2022. AEDV
Descargar PDF
Idiomas
Actas Dermo-Sifiliográficas
Opciones de artículo
Herramientas
es en

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?