Platelet-Rich Plasma for the Treatment of Photodamage of the Skin of the Hands

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Cabrera-Ramírez, A.G. Puebla-Mora, A. González-Ojeda, D. García-Martínez, J.A. Cortés-Lares, A.R. Márquez-Valdéz, G.I. Contreras-Hernández, J. Bracamontes-Blanco, J.A. Saucedo-Ortíz, C. Fuentes-Orozco" "autores" => array:10 [ 0 => array:3 [ "nombre" => "J.O." "apellidos" => "Cabrera-Ramírez" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "a" "identificador" => "aff0005" ] ] ] 1 => array:3 [ "nombre" => "A.G." "apellidos" => "Puebla-Mora" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "b" "identificador" => "aff0010" ] ] ] 2 => array:3 [ "nombre" => "A." "apellidos" => "González-Ojeda" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "c" "identificador" => "aff0015" ] ] ] 3 => array:3 [ "nombre" => "D." "apellidos" => "García-Martínez" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "c" "identificador" => "aff0015" ] ] ] 4 => array:3 [ "nombre" => "J.A." 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"apellidos" => "Fuentes-Orozco" "email" => array:1 [ 0 => "clotilde.fuentes@gmail.com" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "c" "identificador" => "aff0015" ] 1 => array:2 [ "etiqueta" => "*" "identificador" => "cor0005" ] ] ] ] "afiliaciones" => array:3 [ 0 => array:3 [ "entidad" => "Servicio de Cirugía Plástica, Hospital de Especialidades del Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Servicio de Anatomía y Patología, Hospital de Especialidades del Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico" "etiqueta" => "b" "identificador" => "aff0010" ] 2 => array:3 [ "entidad" => "Unidad de Investigación Biomédica 02, Unidad Médica de Alta Especialidad, Hospital de Especialidades, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico" "etiqueta" => "c" "identificador" => "aff0015" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Plasma rico en plaquetas en el tratamiento del fotodaño cutáneo en las manos" ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0010" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 1492 "Ancho" => 950 "Tamanyo" => 432611 ] ] "descripcion" => array:1 [ "en" => "Histologic features of biopsied tissue; hematoxylin and eosin, original magnification ×40. A, After treatments with platelet-rich plasma the number of fibroblasts increased. B, Before treatments." ] ] ] "textoCompleto" => "IntroductionSkin aging is the result of accumulated damage that leads to the gradual biological and functional decline of cells over time (intrinsic aging) or the influence of environmental factors such as smoking, exposure to chemicals or UV radiation (extrinsic aging).<a class="elsevierStyleCrossRef" href="#bib0155">1</a>Constant exposure to UV radiation diminishes the structural function and integrity of the extracellular matrix through collagen degeneration and abnormal elastin structure, leading to the loss of both skin tone and elasticity, causing the signs of aging.<a class="elsevierStyleCrossRef" href="#bib0160">2</a>Platelet activation in response to tissue damage and vascular exposure develops to promote hemostasis and secrete biologically active proteins<a class="elsevierStyleCrossRefs" href="#bib0165">3–6</a> that undertake functions related to chemotaxis, tissue proliferation and differentiation, angiogenesis, and regeneration of the extracellular matrix. A dose–response relationship between platelet concentration and the proliferation of mesenchymal cells, fibroblasts, and type 1 collagen has been observed in vitro.<a class="elsevierStyleCrossRefs" href="#bib0185">7–10</a>Growth factors in platelet-rich plasma (PRP) can stimulate fibroblast activation to regenerate damaged soft and hard tissues.<a class="elsevierStyleCrossRefs" href="#bib0160">2,3</a> These regenerative effects are attributed to the presence of biological factors such as platelet-derived growth factor, ß-transforming growth factor, epidermal growth factor, insulin-like growth factor, and vascular endothelial growth factor, all of which are released locally after PRP is applied.<a class="elsevierStyleCrossRef" href="#bib0205">11</a> Increased dermal and epidermal thickness as well as enhanced numbers and organization of fibroblasts in photodamaged skin indicate that these factors act on cell proliferation and reduce actinic elastosis<a class="elsevierStyleCrossRefs" href="#bib0210">12,13</a> even up to 3 months after application.<a class="elsevierStyleCrossRef" href="#bib0180">6</a>Although PRP is used in a variety of conditions, only a few experimental studies have looked at the effects of PRP stimulation to treat skin aging.<a class="elsevierStyleCrossRefs" href="#bib0180">6,14–17</a>Material and MethodsThis experimental study was carried out between August 1, 2012, and January 31, 2013. After approval by the local medical research review board and ethics committee (file number R-2013-1301-142), we enrolled patients aged between 40 and 60 years who had mild skin photoaging according to the Glogau scale.<a class="elsevierStyleCrossRef" href="#bib0240">18</a> The exclusion criteria were the use of topical treatments in the 6 months before the study, pregnancy, skin infection, a history of platelet dysfunction, use of corticosteroids, smoking, cancer, hemoglobin level less than 10g/dL, or a platelet count less than 105×109/L.Three PRP application sessions were given 30 days apart.Outcomes were assessed on the Glogau scale and the Fitzpatrick wrinkle scale<a class="elsevierStyleCrossRef" href="#bib0245">19</a> and through histologic examination of posttreatment skin biopsies. Patients were asked not to change their routine skin care habits during the study.Method for Obtaining PRPWe extracted autologous venous blood into sterile vacuum collection tubes containing a solution of 3.2% sodium citrate. The tubes were then placed in a digital centrifuge (MS2000). The first cycle was set at 1500rpm for 10minutes to separate blood components (erythrocytes, buffy layer, and plasma). The plasma was then separated into 2 upper layers of platelet-poor plasma and a lower layer of PRP. Using sterile technique, we collected the 2upper fractions from each patient sample into 2 sterile vacuum tubes using 10-mL syringes and 25-gauge, 16-mm hypodermic needles. The same procedure was then used to transfer each of the lower fractions to a third sterile vacuum tube for each sample. The 3 tubes containing the 3 plasma layers from each sample were then centrifuged at 2000rpm for 10minutes.<a class="elsevierStyleCrossRef" href="#bib0250">20</a>Anesthetic TechniqueThe dorsal surface of the hand was disinfected and an antiseptic solution was applied. Using sterile technique we performed radial and ulnar nerve blocks with subcutaneous injections of lidocaine in 2% solution. A total of 3cc was injected proximal to the styloid apophysis of the radius (anatomical snuff box), and 2cc was injected at the ulnar styloid apophysis between the flexor carpi ulnaris tendon and the ulnar artery.Biopsy MethodWe biopsied the most severely photodamaged area with a disposable 2.5-mm punch (Uni-Punch) at 2 moments: immediately before the first treatment application and 28 days after the last application.PRP ApplicationThe upper plasma fractions were activated by adding a 10:1 (10%) solution of calcium gluconate to the tube and shaking. The resulting mixture was extracted to a 100-IU syringe with a 25-gauge, 16-mm hypodermic needle. A No. 11 blade was used to make stab incisions on the dorsal surfaces of the second, third, and fourth interdigital spaces; 17-mm blunt-tipped catheters for lipids were inserted for radial and crossed-pattern injection of the plasma.<a class="elsevierStyleCrossRefs" href="#bib0255">21,22</a> Next, the lower fraction was activated in the same way, and injections of approximately 1.5mL were made into the dermis from the dorsal surface of the hand with a 25-gauge, 16-mm needle. The injections were spaced 1.5to 2mm apart.Clinical EvaluationParticipants were evaluated and classified on the Glogau photoaging scale<a class="elsevierStyleCrossRef" href="#bib0240">18</a> before starting treatment and a month after the last application. The classifications were as follows: type I, early photoaging, no wrinkles; type II, early photoaging, appearance of wrinkles when skin moves; type III, advanced photoaging, wrinkles at rest; type IV, severe photoaging, wrinkles throughout. The patients were also evaluated on the Fitzpatrick wrinkle scale<a class="elsevierStyleCrossRef" href="#bib0245">19</a> as class I, fine wrinkles and mild elastosis; class II, fine to moderately deep wrinkles and moderate elastosis; and class III, severe elastosis with fine to deep wrinkles.HistologyBiopsied tissue samples were fixed with 10% formalin, sectioned (6μm), and stained with hematoxylin-eosin for fibroblasts and nuclei; Masson's trichrome for type I collagen fibers, and periodic acid-Schiff stain for basement membranes of blood vessels.Digital images were taken and analyzed with Image software. Histologic assessment of blood vessels, collagen, and fibroblasts was performed on 10 fields, original magnification ×40; the tissue examined was from a location 2mm below the basement membrane of the epithelium (toward the deep dermis) in areas not adjacent to adnexa. Atypia, or fibroblastic reactivity in the nucleus, and elastosis were recorded as present or absent.Statistical AnalysisResults for qualitative variables were described with absolute frequencies and percentages; they were compared with the Fisher exact test or the χ2 test. Quantitative results were reported with mean (SD) and medians as appropriate; they were compared with the Wilcoxon signed-rank test. P values less than .05 were considered statistically significant.ResultsWe included 20 women, 2 of whom interrupted treatment (1 because of isolated phlebitis of the arm and 1 because of uncontrolled dyslipidemia). The remaining 18 patients finished the study. The mean age was 47.9 (4.3) years.The Fitzpatrick wrinkle scale evaluations improved significantly after the PRP treatments (P<.001). The mean pre- and posttreatment scores were 4.94 (0.80) and 2.78 (0.42), respectively, representing a change in category from class II to class I.Eleven patients were initially classified as type III and 7 as type II according to the Glogau photaging scale. After treatment, 7 of the 11 patients (63.64%) were reclassified as type II (P=.01); the classification for the remaining patients did not change (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>).<elsevierMultimedia ident="fig0005"></elsevierMultimedia>On histologic assessment, we found increases in the number of blood vessels per standardized basement membrane measure and fibroblasts per number of nuclei examined. Papillary dermal collagen had also increased with treatments (<a class="elsevierStyleCrossRefs" href="#fig0010">Figs. 2 and 3</a>).<elsevierMultimedia ident="fig0010"></elsevierMultimedia><elsevierMultimedia ident="fig0015"></elsevierMultimedia>We identified no changes in elastosis or basement membrane structure that could be attributed to PRP treatment, however. Nor did we find atypia in fibroblasts, other than vesicular nuclei and a few nucleoli in relation to slight fibroblastic reactivity.The number of fibroblasts and blood vessels observed and the amount of collagen are shown in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>. Fibroblasts and blood vessels both increased.<elsevierMultimedia ident="tbl0005"></elsevierMultimedia>DiscussionSkin shows the aging process through multiple clinical signs. One of the most important is the development of wrinkles, which gives the skin an undesirable appearance when combined with elastosis, color variations, and keratosis. Underlying these signs are histologic changes.Several studies have shown that intradermal PRP injections can stimulate growth factors, favoring the regeneration of skin cells.<a class="elsevierStyleCrossRefs" href="#bib0265">23,24</a> We saw a considerable increase in the number of fibroblastic nuclei and augmentation of blood vessel basement membranes after PRP treatments. Although the assessed variables changed significantly, the increase in the amount of collagen present was not as marked as in some other studies.<a class="elsevierStyleCrossRefs" href="#bib0180">6,17</a>In one of these studies, histology of skin biopsies after treatments with plasma rich in growth factors showed increased dermal and epidermal thickness and a significant increase in fibroblasts and collagen fibers 3 months after treatment ended.<a class="elsevierStyleCrossRef" href="#bib0180">6</a> It is possible that the timing of the biopsy may affect whether histologic changes can be visualized or not. However, our findings were consistent with a study in which patients reported a change in the severity of wrinkles in spite of the lack of a significant increase in the amount of collagen.<a class="elsevierStyleCrossRef" href="#bib0275">25</a>Fibroblasts are responsible for synthesizing the reticular, collagen, and elastic fibers of the extracellular matrix of tissues; thus, stimulating the production of fibroblasts will enhance the matrix.<a class="elsevierStyleCrossRefs" href="#bib0280">26,27</a> We therefore believe that the processes of tissue repair and regeneration will continue to take place in our patients beyond the moment when we took our measurements. Histologic changes would probably have become significantly more evident in later biopsies. The early timing of the biopsy procedures in our study was therefore a limitation.The proangiogenic factors released by PRP also promote a change in endothelial cell phenotype, favoring proliferation and enabling cells to remodel the extracellular matrix of circulating blood, as well as migrate to the focus of angiogenesis and differentiate to form new blood vessels.<a class="elsevierStyleCrossRefs" href="#bib0190">8,28</a> Cell lines responsible for angiogenesis are also involved in the recognition, repair, and chemotaxis required for regeneration. Angiogenesis could therefore be critical for sustaining the clinical effects of PRP treatment. We suggest that angiogenesis should be assessed in future clinical trials.Some authors have found that topical or subcutaneous application of PRP and growth factors lead to significant changes in the skin.<a class="elsevierStyleCrossRefs" href="#bib0295">29,30</a> These treatments restore elasticity and vitality as well as increase thickness and enhance blood flow, suggesting that PRP and growth factors regulate dermal and epidermal remodeling in facial skin. However, the fact that the mere use of a needle to inject any substance might in itself activate fibroblasts in the skin and stimulate collagen synthesis was mentioned in one study.<a class="elsevierStyleCrossRef" href="#bib0165">3</a> Abuaf et al.<a class="elsevierStyleCrossRef" href="#bib0235">17</a> recently showed that the amount of collagen increased with mesotherapy even without the application of PRP. It would be reasonable, therefore, to expect that our study would demonstrate even greater changes, given the technique we used; we did not see a large effect, however.Because the hands are generally under repeated exposure to sunlight, our findings may be useful for deciding how to treat photodamaged skin in areas that are less exposed. Our review of the literature indicates that this is the first study on the effect of hand skin rejuvenation through PRP treatments. We believe that more such studies should be done to further our understanding of the effects of applying PRP to photodamaged skin.ConclusionPRP treatments reduced signs of skin aging, favored collagen synthesis, and increased the number of fibroblasts and blood vessels in the superficial layer of the dermis.Ethical DisclosuresProtection of human and animal subjectsThe authors declare that the procedures followed adhered to the ethical guidelines of the responsible committee on human experimentation and comply with the Declaration of Helsinki of the World Medical Association.Data confidentialityThe authors declare that they followed their hospitals’ regulations regarding the publication of patient information and that written informed consent for voluntary participation was obtained for all patients.Right to privacy and informed consentThe authors have obtained the informed consent of the patients and/or subjects referred to in this article. The signed forms are in the possession of the corresponding author.Conflicts of InterestThe authors declare that they have no conflicts of interest." "textoCompletoSecciones" => array:1 [ "secciones" => array:12 [ 0 => array:3 [ "identificador" => "xres908296" "titulo" => "Abstract" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abst0005" "titulo" => "Background" ] 1 => array:2 [ "identificador" => "abst0010" "titulo" => "Material and methods" ] 2 => array:2 [ "identificador" => "abst0015" "titulo" => "Results" ] 3 => array:2 [ "identificador" => "abst0020" "titulo" => "Conclusions" ] ] ] 1 => array:2 [ "identificador" => "xpalclavsec888219" "titulo" => "Keywords" ] 2 => array:3 [ "identificador" => "xres908295" "titulo" => "Resumen" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abst0025" "titulo" => "Introducción" ] 1 => array:2 [ "identificador" => "abst0030" "titulo" => "Material y métodos" ] 2 => array:2 [ "identificador" => "abst0035" "titulo" => "Resultados" ] 3 => array:2 [ "identificador" => "abst0040" "titulo" => "Conclusiones" ] ] ] 3 => array:2 [ "identificador" => "xpalclavsec888218" "titulo" => "Palabras clave" ] 4 => array:2 [ "identificador" => "sec0005" "titulo" => "Introduction" ] 5 => array:3 [ "identificador" => "sec0010" "titulo" => "Material and Methods" "secciones" => array:7 [ 0 => array:2 [ "identificador" => "sec0015" "titulo" => "Method for Obtaining PRP" ] 1 => array:2 [ "identificador" => "sec0020" "titulo" => "Anesthetic Technique" ] 2 => array:2 [ "identificador" => "sec0025" "titulo" => "Biopsy Method" ] 3 => array:2 [ "identificador" => "sec0030" "titulo" => "PRP Application" ] 4 => array:2 [ "identificador" => "sec0035" "titulo" => "Clinical Evaluation" ] 5 => array:2 [ "identificador" => "sec0040" "titulo" => "Histology" ] 6 => array:2 [ "identificador" => "sec0045" "titulo" => "Statistical Analysis" ] ] ] 6 => array:2 [ "identificador" => "sec0050" "titulo" => "Results" ] 7 => array:2 [ "identificador" => "sec0055" "titulo" => "Discussion" ] 8 => array:2 [ "identificador" => "sec0060" "titulo" => "Conclusion" ] 9 => array:3 [ "identificador" => "sec0065" "titulo" => "Ethical Disclosures" "secciones" => array:3 [ 0 => array:2 [ "identificador" => "sec0070" "titulo" => "Protection of human and animal subjects" ] 1 => array:2 [ "identificador" => "sec0075" "titulo" => "Data confidentiality" ] 2 => array:2 [ "identificador" => "sec0080" "titulo" => "Right to privacy and informed consent" ] ] ] 10 => array:2 [ "identificador" => "sec0085" "titulo" => "Conflicts of Interest" ] 11 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2016-12-08" "fechaAceptado" => "2017-04-18" "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec888219" "palabras" => array:3 [ 0 => "Photodamage" 1 => "Platelet-rich plasma" 2 => "Growth factors" ] ] ] "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec888218" "palabras" => array:3 [ 0 => "Fotodaño" 1 => "Plasma rico en plaquetas" 2 => "Factores de crecimiento" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "en" => array:3 [ "titulo" => "Abstract" "resumen" => "BackgroundPlatelet-rich plasma (PRP) provides growth factors that stimulate fibroblast activation and induce the synthesis of collagen and other components of the extracellular matrix. The objective of this study was to evaluate the effect of PRP in the treatment of photodamage of the skin of the hands. Material and methodsExperimental study enrolling persons with photoaged skin on the dorsum of the hands (Glogau photoaging scale, type III, or Fitzpatrick wrinkle classification, type II) were included between August 2012 and January 2013. A histological comparison was made of skin biopsies taken before and after the application of PRP to the skin of the dorsum of the hands. ResultsThe mean (SD) age of the 18 women enrolled was 47.9 (4.3) years. Histological analysis showed an increase in the number of fibroblasts (P<.001), number of vessels (P<.001), and collagen density (P=.27). These changes produced significant improvements in the Fitzpatrick wrinkle and elastosis scale (P<.001) and in the Glogau photoaging scale (P=.01). ConclusionsPRP induced a reduction in the manifestations of skin aging, including an improvement in wrinkles and elastosis." "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abst0005" "titulo" => "Background" ] 1 => array:2 [ "identificador" => "abst0010" "titulo" => "Material and methods" ] 2 => array:2 [ "identificador" => "abst0015" "titulo" => "Results" ] 3 => array:2 [ "identificador" => "abst0020" "titulo" => "Conclusions" ] ] ] "es" => array:3 [ "titulo" => "Resumen" "resumen" => "IntroducciónEl plasma rico en plaquetas (PRP) aporta factores de crecimiento que inducen la síntesis de colágeno y otros componentes de la matriz extracelular, y estimula la activación de fibroblastos. El objetivo de este estudio es evaluar el efecto del PRP en el tratamiento del fotodaño en manos. Material y métodosEstudio de tipo experimental. Se incluyeron individuos con envejecimiento en el dorso de las manos, de tipo 3 en la escala de fotodaño de Glogau o de clase II de la clasificación de arrugas y elastosis de Fitzpatrick, entre agosto de 2012 y enero de 2013. Se aplicó PRP en la piel del dorso de las manos y se tomaron biopsias cutáneas antes y después del tratamiento para la comparación histológica. ResultadosSe trataron 18 mujeres con una edad media de 47,9 ± 4,3 años. El análisis histológico demostró un incremento en el número de fibroblastos (p=0,000), número de vasos (p=0,000) y cantidad de colágeno (p=0,27), representado como un cambio en la escala de clasificación de arrugas y elastosis de Fitzpatrick (p<0,001) y en la escala de fotodaño de Glogau (p=0,01) ConclusionesEl PRP indujo una reducción en los signos y síntomas del envejecimiento cutáneo, con mejoría en las arrugas y elastosis." "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abst0025" "titulo" => "Introducción" ] 1 => array:2 [ "identificador" => "abst0030" "titulo" => "Material y métodos" ] 2 => array:2 [ "identificador" => "abst0035" "titulo" => "Resultados" ] 3 => array:2 [ "identificador" => "abst0040" "titulo" => "Conclusiones" ] ] ] ] "NotaPie" => array:1 [ 0 => array:2 [ "etiqueta" => "☆" "nota" => "Please cite this article as: Cabrera-Ramírez JO, Puebla-Mora AG, González-Ojeda A, García-Martínez D, Cortés-Lares JA, Márquez-Valdés AR, et al. Plasma rico en plaquetas en el tratamiento del fotodaño cutáneo en las manos. Actas Dermosifiliogr. 2017;108:746–751." ] ] "multimedia" => array:4 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1422 "Ancho" => 950 "Tamanyo" => 187656 ] ] "descripcion" => array:1 [ "en" => "Dorsal surfaces of treated hands. A, Before treatments with platelet-rich plasma. B, After treatments." ] ] 1 => array:7 [ "identificador" => "fig0010" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 1492 "Ancho" => 950 "Tamanyo" => 432611 ] ] "descripcion" => array:1 [ "en" => "Histologic features of biopsied tissue; hematoxylin and eosin, original magnification ×40. A, After treatments with platelet-rich plasma the number of fibroblasts increased. B, Before treatments." ] ] 2 => array:7 [ "identificador" => "fig0015" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 1435 "Ancho" => 951 "Tamanyo" => 452283 ] ] "descripcion" => array:1 [ "en" => "Collagen fibers; Masson's trichrome, original magnification ×40. A, Before treatments with platelet-rich plasma. B, After treatments collagen had increased." ] ] 3 => array:8 [ "identificador" => "tbl0005" "etiqueta" => "Table 1" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at1" "detalle" => "Table " "rol" => "short" ] ] "tabla" => array:2 [ "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head " rowspan="2" align="left" valign="top" scope="col" style="border-bottom: 2px solid black"></th><th class="td" title="table-head " colspan="3" align="center" valign="top" scope="col" style="border-bottom: 2px solid black">Before Treatments</th><th class="td" title="table-head " colspan="3" align="center" valign="top" scope="col" style="border-bottom: 2px solid black">After Treatments</th><th class="td" title="table-head " rowspan="2" align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Z Score</th><th class="td" title="table-head " rowspan="2" align="left" valign="top" scope="col" style="border-bottom: 2px solid black">P<a class="elsevierStyleCrossRef" href="#tblfn0005">a</a></th></tr><tr title="table-row"><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">No. \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Mean (SD) \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Median \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">No. \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Mean (SD) \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Median \t\t\t\t\t\t\n \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Fibroblasts/250μm2 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">18 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">2.78 (0.42) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">3 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">18 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">4.94 (0.80) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">5 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">−3.77 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">.000 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Mean fraction of the area with collagen \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">18 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">60.67 (2.78) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">62 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">18 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">63 (1.60) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">63 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">−2.20 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">.27 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">No. of blood vessels/250μm2 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">18 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">6.72 (1.80) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">6.5 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">18 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">11.5 (2.3) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">11.5 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">−3.49 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">.000 \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab1527657.png" ] ] ] "notaPie" => array:1 [ 0 => array:3 [ "identificador" => "tblfn0005" "etiqueta" => "a" "nota" => "Wilcoxon signed-rank test." ] ] ] "descripcion" => array:1 [ "en" => "Histologic Findings Before and After Platelet-Rich Plasma Treatments." ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:30 [ 0 => array:3 [ "identificador" => "bib0155" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Fifty years of skin aging" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "M. Yaar" 1 => "M.S. Eller" 2 => "B.A. Gilchrest" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "J Invest Dermatol Symposium Proceedings." "fecha" => "2002" "volumen" => "7" "paginaInicial" => "51" "paginaFinal" => "58" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0160" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Applications of platelet-rich fibrin matrix in facial plastic surgery" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "A.P. Sclafani" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1055/s-0029-1242033" "Revista" => array:7 [ "tituloSerie" => "Facial Plast Surg." "fecha" => "2009" "volumen" => "25" "paginaInicial" => "270" "paginaFinal" => "276" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19924600" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S0140673615003402" "estado" => "S300" "issn" => "01406736" ] ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib0165" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Can platelet-rich plasma be used for skin rejuvenation? Evaluation of effects of platelet-rich plasma on human dermal fibroblast" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "D.H. Kim" 1 => "Y.J. Je" 2 => "C.D. Kim" 3 => "Y.H. Lee" 4 => "Y.J. Seo" 5 => "J.H. Lee" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.5021/ad.2011.23.4.424" "Revista" => array:6 [ "tituloSerie" => "Ann Dermatol." "fecha" => "2011" "volumen" => "23" "paginaInicial" => "424" "paginaFinal" => "431" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22148008" "web" => "Medline" ] ] ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib0170" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The potential impact of the preparation rich in growth factors (PRGF) in different medical fields" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "E. Anitua" 1 => "M. Sanchez" 2 => "G. Orive" 3 => "I. Andía" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.biomaterials.2007.06.037" "Revista" => array:6 [ "tituloSerie" => "Biomaterials." "fecha" => "2007" "volumen" => "28" "paginaInicial" => "4551" "paginaFinal" => "4560" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17659771" "web" => "Medline" ] ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib0175" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Systematic review of the use of platelet-rich plasma in aesthetic dermatology" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "M.S. Leo" 1 => "A.S. Kumar" 2 => "R. Kirit" 3 => "R. Konathan" 4 => "R.K. Sivamani" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/jocd.12167" "Revista" => array:6 [ "tituloSerie" => "J Cosmet Dermatol" "fecha" => "2015" "volumen" => "14" "paginaInicial" => "315" "paginaFinal" => "323" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26205133" "web" => "Medline" ] ] ] ] ] ] ] ] 5 => array:3 [ "identificador" => "bib0180" "etiqueta" => "6" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Benefits of plasma rich in growth factors (PRGF) in skin photodamage: Clinical response and histological assessment" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "B. Díaz-Ley" 1 => "J. Cuevas" 2 => "L. Alonso-Castro" 3 => "M.I. Calvo" 4 => "L. Ríos-Buceta" 5 => "G. Orive" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/dth.12228" "Revista" => array:7 [ "tituloSerie" => "Dermatol Ther." "fecha" => "2015" "volumen" => "28" "paginaInicial" => "258" "paginaFinal" => "263" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/25864463" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S0735109713060294" "estado" => "S300" "issn" => "07351097" ] ] ] ] ] ] ] 6 => array:3 [ "identificador" => "bib0185" "etiqueta" => "7" "referencia" => array:1 [ 0 => array:1 [ "referenciaCompleta" => "Online Archive of International cellular medicine society. Section VIII Platelet rich plasma (PRP) guidelines 2011 [cited 2014 Oct 3). Available from: <a href="http://www.cellmedicinesociety.org/attachments/370_Section%2010%20-%20Platelet%20Rich%20Plasma%20(PRP)%20Guidelines.pdf">http://www.cellmedicinesociety.org/attachments/370_Section%2010%20-%20Platelet%20Rich%20Plasma%20(PRP)%20Guidelines.pdf</a>." ] ] ] 7 => array:3 [ "identificador" => "bib0190" "etiqueta" => "8" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The use of plasma-rich growth factors (PRGF) in oral surgery" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "E. Anitua" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Pract Proced Aesthet Dent." "fecha" => "2001" "volumen" => "13" "paginaInicial" => "487" "paginaFinal" => "493" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11544821" "web" => "Medline" ] ] ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bib0195" "etiqueta" => "9" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Plasma rico en plaquetas; una revisión bibliográfica" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "T. Beca" 1 => "G. Hernández" 2 => "S. Morante" 3 => "A. Bascones" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Av Periodon Implantol." "fecha" => "2007" "volumen" => "19" "paginaInicial" => "39" "paginaFinal" => "52" ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bib0200" "etiqueta" => "10" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The effects of autologous platelet gel on wound healing" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "J. Henderson" 1 => "C. Cupp" 2 => "E. Ross" 3 => "P. Shick" 4 => "M. Keefe" 5 => "D. Wester" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Ear Nose Throat J." "fecha" => "2003" "volumen" => "82" "paginaInicial" => "598" "paginaFinal" => "602" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/14503096" "web" => "Medline" ] ] ] ] ] ] ] ] 10 => array:3 [ "identificador" => "bib0205" "etiqueta" => "11" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Platelet-rich plasma combined with fractional laser therapy for skin rejuvenation" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "M. Shin" 1 => "J. Lee" 2 => "S. Lee" 3 => "N. Kim" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/j.1524-4725.2011.02280.x" "Revista" => array:7 [ "tituloSerie" => "Dermatol Surg." "fecha" => "2012" "volumen" => "38" "paginaInicial" => "623" "paginaFinal" => "630" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22288389" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S0140673612617280" "estado" => "S300" "issn" => "01406736" ] ] ] ] ] ] ] 11 => array:3 [ "identificador" => "bib0210" "etiqueta" => "12" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Autologous platelet-rich plasma: A potential therapeutic tool for promoting hair growth" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "Z. Li" 1 => "H. Choi" 2 => "D. Choi" 3 => "K. Sohn" 4 => "M. Im" 5 => "Y. Seo" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/j.1524-4725.2012.02394.x" "Revista" => array:6 [ "tituloSerie" => "Dermatol Surg." "fecha" => "2012" "volumen" => "38" "paginaInicial" => "1040" "paginaFinal" => "1046" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22455565" "web" => "Medline" ] ] ] ] ] ] ] ] 12 => array:3 [ "identificador" => "bib0215" "etiqueta" => "13" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Photoaging, mechanisms and repair" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "J. Rabe" 1 => "A. Mamelak" 2 => "P. McElgunn" 3 => "W. Morison" 4 => "D. Sauder" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.jaad.2005.05.010" "Revista" => array:6 [ "tituloSerie" => "J Am Acad Dermatol." "fecha" => "2006" "volumen" => "55" "paginaInicial" => "1" "paginaFinal" => "13" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16781287" "web" => "Medline" ] ] ] ] ] ] ] ] 13 => array:3 [ "identificador" => "bib0220" "etiqueta" => "14" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Current applications of platelet gels in facial plastic surgery" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "S. Bhanot" 1 => "J.C. Alex" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1055/s-2002-19824" "Revista" => array:6 [ "tituloSerie" => "Facial Plast Surg." "fecha" => "2002" "volumen" => "18" "paginaInicial" => "27" "paginaFinal" => "33" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11823930" "web" => "Medline" ] ] ] ] ] ] ] ] 14 => array:3 [ "identificador" => "bib0225" "etiqueta" => "15" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Gene therapy in wound repair and regeneration" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "E. Yao" 1 => "E. Eriksson" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Wound Repair Regen" "fecha" => "2000" "volumen" => "8" "paginaInicial" => "4443" "paginaFinal" => "4451" ] ] ] ] ] ] 15 => array:3 [ "identificador" => "bib0230" "etiqueta" => "16" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Terapia de bioestimulación con plasma rico en plaquetas para el envejecimiento cutáneo" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "H.M. Escobar" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:3 [ "tituloSerie" => "Rev Argent Dermatol." "fecha" => "2012" "volumen" => "93" ] ] ] ] ] ] 16 => array:3 [ "identificador" => "bib0235" "etiqueta" => "17" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Histologic evidence of new collagen formulation using platelet rich plasma in skin rejuvenation: A prospective controlled clinical study" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "O.K. Abuaf" 1 => "H. Yildiz" 2 => "H. Baloglu" 3 => "M.E. Bilgili" 4 => "H.A. Simsek" 5 => "B. Dogan" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.5021/ad.2016.28.6.718" "Revista" => array:6 [ "tituloSerie" => "Ann Dermatol" "fecha" => "2016" "volumen" => "28" "paginaInicial" => "718" "paginaFinal" => "724" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/27904271" "web" => "Medline" ] ] ] ] ] ] ] ] 17 => array:3 [ "identificador" => "bib0240" "etiqueta" => "18" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Aesthetic and anatomic analysis of the aging skin" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "R.G. Glogau" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:7 [ "tituloSerie" => "Semin Cutan Med Surg." "fecha" => "1996" "volumen" => "15" "paginaInicial" => "134" "paginaFinal" => "138" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8948530" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S0140673609603184" "estado" => "S300" "issn" => "01406736" ] ] ] ] ] ] ] 18 => array:3 [ "identificador" => "bib0245" "etiqueta" => "19" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Pulsed carbon dioxide laser resurfacing of photo-aged facial skin" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "R.E. Fitzpatrick" 1 => "M.P. Goldman" 2 => "N.M. Satur" 3 => "W.D. Tope" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:7 [ "tituloSerie" => "Arch Dermatol" "fecha" => "1996" "volumen" => "132" "paginaInicial" => "395" "paginaFinal" => "402" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8629842" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S0140673613610971" "estado" => "S300" "issn" => "01406736" ] ] ] ] ] ] ] 19 => array:3 [ "identificador" => "bib0250" "etiqueta" => "20" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Relevant aspects of centrifugation step in the preparation of platelet-rich plasma" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "A.G. Perez" 1 => "J.F.S. Lana" 2 => "A.A. Rodrigues" 3 => "A.C.M. Luzo" 4 => "W.D. Belangero" 5 => "M.H.A. Santana" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:2 [ "tituloSerie" => "ISRN Hematol" "fecha" => "2014" ] ] ] ] ] ] 20 => array:3 [ "identificador" => "bib0255" "etiqueta" => "21" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Lipoaugmentation for aging hands: A comparision of the longevity and aesthetic results of centrifuged versus noncentrifuged fat" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "K.J. Butterwick" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Dermatol Surg" "fecha" => "2002" "volumen" => "28" "paginaInicial" => "987" "paginaFinal" => "991" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12460290" "web" => "Medline" ] ] ] ] ] ] ] ] 21 => array:3 [ "identificador" => "bib0260" "etiqueta" => "22" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Hand rejuvenation with structural fat grafting" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "S.R. Coleman" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1097/01.PRS.0000033936.43357.08" "Revista" => array:6 [ "tituloSerie" => "Plast Reconstr Surg" "fecha" => "2002" "volumen" => "110" "paginaInicial" => "1731" "paginaFinal" => "1744" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12447057" "web" => "Medline" ] ] ] ] ] ] ] ] 22 => array:3 [ "identificador" => "bib0265" "etiqueta" => "23" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Proliferation-promoting effect of platelet-rich plasma on human adipose–derived stem cells and human dermal fibroblasts" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "N. Kakudo" 1 => "T. Minakata" 2 => "T. Mitsui" 3 => "S. Kushida" 4 => "F.Z. Notodihardjo" 5 => "K. Kusumoto" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Plast Reconstr Surg." "fecha" => "2008" "volumen" => "11" "paginaInicial" => "1352" "paginaFinal" => "1360" ] ] ] ] ] ] 23 => array:3 [ "identificador" => "bib0270" "etiqueta" => "24" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Plasma rico en plaquetas ¿Herramienta versátil de la medicina regenerativa?" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "P. Carrillo" 1 => "A. Gonzalez" 2 => "S. Macias" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Cir Cir." "fecha" => "2013" "volumen" => "81" "paginaInicial" => "74" "paginaFinal" => "82" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23461926" "web" => "Medline" ] ] ] ] ] ] ] ] 24 => array:3 [ "identificador" => "bib0275" "etiqueta" => "25" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Assessment of the efficacy and safety of single platelet-rich plasma injection on different types and grades of facial wrinkles" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "N.Y. Elnehrawy" 1 => "Z.A. Ibrahim" 2 => "A.M. Eltoukhy" 3 => "H.M. Nagy" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/jocd.12258" "Revista" => array:2 [ "tituloSerie" => "J Cosmet Dermatol" "fecha" => "2016" ] ] ] ] ] ] 25 => array:3 [ "identificador" => "bib0280" "etiqueta" => "26" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Platelet-rich plasma: Support for its use in wound healing" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "K. Lacci" 1 => "A. Dardik" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:7 [ "tituloSerie" => "Yale J Biol Med." "fecha" => "2010" "volumen" => "83" "paginaInicial" => "1" "paginaFinal" => "9" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20351977" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S0735109714068363" "estado" => "S300" "issn" => "07351097" ] ] ] ] ] ] ] 26 => array:3 [ "identificador" => "bib0285" "etiqueta" => "27" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Plasma rico en plaquetas en cirugía estética" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "P. Arquero" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Cir Estet Plas." "fecha" => "2009" "volumen" => "5" "paginaInicial" => "42" "paginaFinal" => "48" ] ] ] ] ] ] 27 => array:3 [ "identificador" => "bib0290" "etiqueta" => "28" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Reversal of photodamage with topical growth factors: A pilot study" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "R.E. Fitzpatrick" 1 => "E.F. Rostan" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:4 [ "tituloSerie" => "J Cosmet Laser Ther" "fecha" => "2003" "volumen" => "5" "paginaInicial" => "25e34" ] ] ] ] ] ] 28 => array:3 [ "identificador" => "bib0295" "etiqueta" => "29" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Improvement in the appearance of wrinkles with topical transforming growth factor beta and 1-ascorbic acid" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "M. Ehrlich" 1 => "J. Rao" 2 => "A. Pabby" 3 => "M.P. Goldman" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/j.1524-4725.2006.32132.x" "Revista" => array:6 [ "tituloSerie" => "Dermatol Surg" "fecha" => "2006" "volumen" => "32" "paginaInicial" => "618" "paginaFinal" => "625" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16706755" "web" => "Medline" ] ] ] ] ] ] ] ] 29 => array:3 [ "identificador" => "bib0300" "etiqueta" => "30" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Efficacy of novel skin cream containing mixture of human growth factors" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "M.H. Gold" 1 => "M.P. Goldman" 2 => "J. Biron" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "J Drugs Dermatol." "fecha" => "2007" "volumen" => "6" "paginaInicial" => "197" "paginaFinal" => "201" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17373178" "web" => "Medline" ] ] ] ] ] ] ] ] ] ] ] ] ] "idiomaDefecto" => "en" "url" => "/15782190/0000010800000008/v1_201710010015/S157821901730238X/v1_201710010015/en/main.assets" "Apartado" => array:4 [ "identificador" => "6256" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Original article" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/15782190/0000010800000008/v1_201710010015/S157821901730238X/v1_201710010015/en/main.pdf?idApp=UINPBA000044&text.app=https://actasdermo.org/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S157821901730238X?idApp=UINPBA000044"]

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Platelet-Rich Plasma for the Treatment of Photodamage of the Skin of the Hands

Plasma rico en plaquetas en el tratamiento del fotodaño cutáneo en las manos

J.O. Cabrera-Ramíreza, A.G. Puebla-Morab, A. González-Ojedac, D. García-Martínezc, J.A. Cortés-Laresc, A.R. Márquez-Valdézc, G.I. Contreras-Hernándezc, J. Bracamontes-Blancoa, J.A. Saucedo-Ortíza, C. Fuentes-Orozcoc,

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clotilde.fuentes@gmail.com

Corresponding author.

a Servicio de Cirugía Plástica, Hospital de Especialidades del Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico

b Servicio de Anatomía y Patología, Hospital de Especialidades del Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico

c Unidad de Investigación Biomédica 02, Unidad Médica de Alta Especialidad, Hospital de Especialidades, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico

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tissue proliferation and differentiation&#44; angiogenesis&#44; and regeneration of the extracellular matrix&#46; A dose&#8211;response relationship between platelet concentration and the proliferation of mesenchymal cells&#44; fibroblasts&#44; and type 1 collagen has been observed in vitro&#46;<a class="elsevierStyleCrossRefs" href="#bib0185"><span class="elsevierStyleSup">7&#8211;10</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">Growth factors in platelet-rich plasma &#40;PRP&#41; can stimulate fibroblast activation to regenerate damaged soft and hard tissues&#46;<a class="elsevierStyleCrossRefs" href="#bib0160"><span class="elsevierStyleSup">2&#44;3</span></a> These regenerative effects are attributed to the presence of biological factors such as platelet-derived growth factor&#44; &#223;-transforming growth factor&#44; epidermal growth factor&#44; insulin-like growth factor&#44; and vascular endothelial growth factor&#44; all of which are released locally after PRP is applied&#46;<a class="elsevierStyleCrossRef" href="#bib0205"><span class="elsevierStyleSup">11</span></a> Increased dermal and epidermal thickness as well as enhanced numbers and organization of fibroblasts in photodamaged skin indicate that these factors act on cell proliferation and reduce actinic elastosis<a class="elsevierStyleCrossRefs" href="#bib0210"><span class="elsevierStyleSup">12&#44;13</span></a> even up to 3 months after application&#46;<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">6</span></a></p><p id="par0025" class="elsevierStylePara elsevierViewall">Although PRP is used in a variety of conditions&#44; only a few experimental studies have looked at the effects of PRP stimulation to treat skin aging&#46;<a class="elsevierStyleCrossRefs" href="#bib0180"><span class="elsevierStyleSup">6&#44;14&#8211;17</span></a></p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Material and Methods</span><p id="par0030" class="elsevierStylePara elsevierViewall">This experimental study was carried out between August 1&#44; 2012&#44; and January 31&#44; 2013&#46; After approval by the local medical research review board and ethics committee &#40;file number R-2013-1301-142&#41;&#44; we enrolled patients aged between 40 and 60 years who had mild skin photoaging according to the Glogau scale&#46;<a class="elsevierStyleCrossRef" href="#bib0240"><span class="elsevierStyleSup">18</span></a> The exclusion criteria were the use of topical treatments in the 6 months before the study&#44; pregnancy&#44; skin infection&#44; a history of platelet dysfunction&#44; use of corticosteroids&#44; smoking&#44; cancer&#44; hemoglobin level less than 10<span class="elsevierStyleHsp" style=""></span>g&#47;dL&#44; or a platelet count less than 105<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">9</span>&#47;L&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">Three PRP application sessions were given 30 days apart&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">Outcomes were assessed on the Glogau scale and the Fitzpatrick wrinkle scale<a class="elsevierStyleCrossRef" href="#bib0245"><span class="elsevierStyleSup">19</span></a> and through histologic examination of posttreatment skin biopsies&#46; Patients were asked not to change their routine skin care habits during the study&#46;</p><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Method for Obtaining PRP</span><p id="par0045" class="elsevierStylePara elsevierViewall">We extracted autologous venous blood into sterile vacuum collection tubes containing a solution of 3&#46;2&#37; sodium citrate&#46; The tubes were then placed in a digital centrifuge &#40;MS<span class="elsevierStyleHsp" style=""></span>2000&#41;&#46; The first cycle was set at 1500<span class="elsevierStyleHsp" style=""></span>rpm for 10<span class="elsevierStyleHsp" style=""></span>minutes to separate blood components &#40;erythrocytes&#44; buffy layer&#44; and plasma&#41;&#46; The plasma was then separated into 2 upper layers of platelet-poor plasma and a lower layer of PRP&#46; Using sterile technique&#44; we collected the 2<span class="elsevierStyleHsp" style=""></span>upper fractions from each patient sample into 2 sterile vacuum tubes using 10-mL syringes and 25-gauge&#44; 16-mm hypodermic needles&#46; The same procedure was then used to transfer each of the lower fractions to a third sterile vacuum tube for each sample&#46; The 3 tubes containing the 3 plasma layers from each sample were then centrifuged at 2000<span class="elsevierStyleHsp" style=""></span>rpm for 10<span class="elsevierStyleHsp" style=""></span>minutes&#46;<a class="elsevierStyleCrossRef" href="#bib0250"><span class="elsevierStyleSup">20</span></a></p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Anesthetic Technique</span><p id="par0050" class="elsevierStylePara elsevierViewall">The dorsal surface of the hand was disinfected and an antiseptic solution was applied&#46; Using sterile technique we performed radial and ulnar nerve blocks with subcutaneous injections of lidocaine in 2&#37; solution&#46; A total of 3<span class="elsevierStyleHsp" style=""></span>cc was injected proximal to the styloid apophysis of the radius &#40;anatomical snuff box&#41;&#44; and 2<span class="elsevierStyleHsp" style=""></span>cc was injected at the ulnar styloid apophysis between the flexor carpi ulnaris tendon and the ulnar artery&#46;</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Biopsy Method</span><p id="par0055" class="elsevierStylePara elsevierViewall">We biopsied the most severely photodamaged area with a disposable 2&#46;5-mm punch &#40;Uni-Punch&#41; at 2 moments&#58; immediately before the first treatment application and 28 days after the last application&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">PRP Application</span><p id="par0060" class="elsevierStylePara elsevierViewall">The upper plasma fractions were activated by adding a 10&#58;1 &#40;10&#37;&#41; solution of calcium gluconate to the tube and shaking&#46; The resulting mixture was extracted to a 100-IU syringe with a 25-gauge&#44; 16-mm hypodermic needle&#46; A No&#46; 11 blade was used to make stab incisions on the dorsal surfaces of the second&#44; third&#44; and fourth interdigital spaces&#59; 17-mm blunt-tipped catheters for lipids were inserted for radial and crossed-pattern injection of the plasma&#46;<a class="elsevierStyleCrossRefs" href="#bib0255"><span class="elsevierStyleSup">21&#44;22</span></a> Next&#44; the lower fraction was activated in the same way&#44; and injections of approximately 1&#46;5<span class="elsevierStyleHsp" style=""></span>mL were made into the dermis from the dorsal surface of the hand with a 25-gauge&#44; 16-mm needle&#46; The injections were spaced 1&#46;5<span class="elsevierStyleHsp" style=""></span>to 2<span class="elsevierStyleHsp" style=""></span>mm apart&#46;</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Clinical Evaluation</span><p id="par0065" class="elsevierStylePara elsevierViewall">Participants were evaluated and classified on the Glogau photoaging scale<a class="elsevierStyleCrossRef" href="#bib0240"><span class="elsevierStyleSup">18</span></a> before starting treatment and a month after the last application&#46; The classifications were as follows&#58; type I&#44; early photoaging&#44; no wrinkles&#59; type II&#44; early photoaging&#44; appearance of wrinkles when skin moves&#59; type III&#44; advanced photoaging&#44; wrinkles at rest&#59; type IV&#44; severe photoaging&#44; wrinkles throughout&#46; The patients were also evaluated on the Fitzpatrick wrinkle scale<a class="elsevierStyleCrossRef" href="#bib0245"><span class="elsevierStyleSup">19</span></a> as class I&#44; fine wrinkles and mild elastosis&#59; class II&#44; fine to moderately deep wrinkles and moderate elastosis&#59; and class III&#44; severe elastosis with fine to deep wrinkles&#46;</p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Histology</span><p id="par0070" class="elsevierStylePara elsevierViewall">Biopsied tissue samples were fixed with 10&#37; formalin&#44; sectioned &#40;6<span class="elsevierStyleHsp" style=""></span>&#956;m&#41;&#44; and stained with hematoxylin-eosin for fibroblasts and nuclei&#59; Masson&#39;s trichrome for type I collagen fibers&#44; and periodic acid-Schiff stain for basement membranes of blood vessels&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">Digital images were taken and analyzed with Image software&#46; Histologic assessment of blood vessels&#44; collagen&#44; and fibroblasts was performed on 10 fields&#44; original magnification &#215;40&#59; the tissue examined was from a location 2<span class="elsevierStyleHsp" style=""></span>mm below the basement membrane of the epithelium &#40;toward the deep dermis&#41; in areas not adjacent to adnexa&#46; Atypia&#44; or fibroblastic reactivity in the nucleus&#44; and elastosis were recorded as present or absent&#46;</p></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Statistical Analysis</span><p id="par0080" class="elsevierStylePara elsevierViewall">Results for qualitative variables were described with absolute frequencies and percentages&#59; they were compared with the Fisher exact test or the &#967;<span class="elsevierStyleSup">2</span> test&#46; Quantitative results were reported with mean &#40;SD&#41; and medians as appropriate&#59; they were compared with the Wilcoxon signed-rank test&#46; <span class="elsevierStyleItalic">P</span> values less than &#46;05 were considered statistically significant&#46;</p></span></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Results</span><p id="par0085" class="elsevierStylePara elsevierViewall">We included 20 women&#44; 2 of whom interrupted treatment &#40;1 because of isolated phlebitis of the arm and 1 because of uncontrolled dyslipidemia&#41;&#46; The remaining 18 patients finished the study&#46; The mean age was 47&#46;9 &#40;4&#46;3&#41; years&#46;</p><p id="par0090" class="elsevierStylePara elsevierViewall">The Fitzpatrick wrinkle scale evaluations improved significantly after the PRP treatments &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>&#46;001&#41;&#46; The mean pre- and posttreatment scores were 4&#46;94 &#40;0&#46;80&#41; and 2&#46;78 &#40;0&#46;42&#41;&#44; respectively&#44; representing a change in category from class II to class I&#46;</p><p id="par0095" class="elsevierStylePara elsevierViewall">Eleven patients were initially classified as type III and 7 as type II according to the Glogau photaging scale&#46; After treatment&#44; 7 of the 11 patients &#40;63&#46;64&#37;&#41; were reclassified as type II &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;01&#41;&#59; the classification for the remaining patients did not change &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>&#41;&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0100" class="elsevierStylePara elsevierViewall">On histologic assessment&#44; we found increases in the number of blood vessels per standardized basement membrane measure and fibroblasts per number of nuclei examined&#46; Papillary dermal collagen had also increased with treatments &#40;<a class="elsevierStyleCrossRefs" href="#fig0010">Figs&#46; 2 and 3</a>&#41;&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia><elsevierMultimedia ident="fig0015"></elsevierMultimedia><p id="par0105" class="elsevierStylePara elsevierViewall">We identified no changes in elastosis or basement membrane structure that could be attributed to PRP treatment&#44; however&#46; Nor did we find atypia in fibroblasts&#44; other than vesicular nuclei and a few nucleoli in relation to slight fibroblastic reactivity&#46;</p><p id="par0110" class="elsevierStylePara elsevierViewall">The number of fibroblasts and blood vessels observed and the amount of collagen are shown in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#46; Fibroblasts and blood vessels both increased&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">Discussion</span><p id="par0115" class="elsevierStylePara elsevierViewall">Skin shows the aging process through multiple clinical signs&#46; One of the most important is the development of wrinkles&#44; which gives the skin an undesirable appearance when combined with elastosis&#44; color variations&#44; and keratosis&#46; Underlying these signs are histologic changes&#46;</p><p id="par0120" class="elsevierStylePara elsevierViewall">Several studies have shown that intradermal PRP injections can stimulate growth factors&#44; favoring the regeneration of skin cells&#46;<a class="elsevierStyleCrossRefs" href="#bib0265"><span class="elsevierStyleSup">23&#44;24</span></a> We saw a considerable increase in the number of fibroblastic nuclei and augmentation of blood vessel basement membranes after PRP treatments&#46; Although the assessed variables changed significantly&#44; the increase in the amount of collagen present was not as marked as in some other studies&#46;<a class="elsevierStyleCrossRefs" href="#bib0180"><span class="elsevierStyleSup">6&#44;17</span></a></p><p id="par0125" class="elsevierStylePara elsevierViewall">In one of these studies&#44; histology of skin biopsies after treatments with plasma rich in growth factors showed increased dermal and epidermal thickness and a significant increase in fibroblasts and collagen fibers 3 months after treatment ended&#46;<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">6</span></a> It is possible that the timing of the biopsy may affect whether histologic changes can be visualized or not&#46; However&#44; our findings were consistent with a study in which patients reported a change in the severity of wrinkles in spite of the lack of a significant increase in the amount of collagen&#46;<a class="elsevierStyleCrossRef" href="#bib0275"><span class="elsevierStyleSup">25</span></a></p><p id="par0130" class="elsevierStylePara elsevierViewall">Fibroblasts are responsible for synthesizing the reticular&#44; collagen&#44; and elastic fibers of the extracellular matrix of tissues&#59; thus&#44; stimulating the production of fibroblasts will enhance the matrix&#46;<a class="elsevierStyleCrossRefs" href="#bib0280"><span class="elsevierStyleSup">26&#44;27</span></a> We therefore believe that the processes of tissue repair and regeneration will continue to take place in our patients beyond the moment when we took our measurements&#46; Histologic changes would probably have become significantly more evident in later biopsies&#46; The early timing of the biopsy procedures in our study was therefore a limitation&#46;</p><p id="par0135" class="elsevierStylePara elsevierViewall">The proangiogenic factors released by PRP also promote a change in endothelial cell phenotype&#44; favoring proliferation and enabling cells to remodel the extracellular matrix of circulating blood&#44; as well as migrate to the focus of angiogenesis and differentiate to form new blood vessels&#46;<a class="elsevierStyleCrossRefs" href="#bib0190"><span class="elsevierStyleSup">8&#44;28</span></a> Cell lines responsible for angiogenesis are also involved in the recognition&#44; repair&#44; and chemotaxis required for regeneration&#46; Angiogenesis could therefore be critical for sustaining the clinical effects of PRP treatment&#46; We suggest that angiogenesis should be assessed in future clinical trials&#46;</p><p id="par0140" class="elsevierStylePara elsevierViewall">Some authors have found that topical or subcutaneous application of PRP and growth factors lead to significant changes in the skin&#46;<a class="elsevierStyleCrossRefs" href="#bib0295"><span class="elsevierStyleSup">29&#44;30</span></a> These treatments restore elasticity and vitality as well as increase thickness and enhance blood flow&#44; suggesting that PRP and growth factors regulate dermal and epidermal remodeling in facial skin&#46; However&#44; the fact that the mere use of a needle to inject any substance might in itself activate fibroblasts in the skin and stimulate collagen synthesis was mentioned in one study&#46;<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">3</span></a> Abuaf et al&#46;<a class="elsevierStyleCrossRef" href="#bib0235"><span class="elsevierStyleSup">17</span></a> recently showed that the amount of collagen increased with mesotherapy even without the application of PRP&#46; It would be reasonable&#44; therefore&#44; to expect that our study would demonstrate even greater changes&#44; given the technique we used&#59; we did not see a large effect&#44; however&#46;</p><p id="par0145" class="elsevierStylePara elsevierViewall">Because the hands are generally under repeated exposure to sunlight&#44; our findings may be useful for deciding how to treat photodamaged skin in areas that are less exposed&#46; Our review of the literature indicates that this is the first study on the effect of hand skin rejuvenation through PRP treatments&#46; We believe that more such studies should be done to further our understanding of the effects of applying PRP to photodamaged skin&#46;</p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0120">Conclusion</span><p id="par0150" class="elsevierStylePara elsevierViewall">PRP treatments reduced signs of skin aging&#44; favored collagen synthesis&#44; and increased the number of fibroblasts and blood vessels in the superficial layer of the dermis&#46;</p></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0125">Ethical Disclosures</span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0130">Protection of human and animal subjects</span><p id="par0155" class="elsevierStylePara elsevierViewall">The authors declare that the procedures followed adhered to the ethical guidelines of the responsible committee on human experimentation and comply with the Declaration of Helsinki of the World Medical Association&#46;</p></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0135">Data confidentiality</span><p id="par0160" class="elsevierStylePara elsevierViewall">The authors declare that they followed their hospitals&#8217; regulations regarding the publication of patient information and that written informed consent for voluntary participation was obtained for all patients&#46;</p></span><span id="sec0080" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0140">Right to privacy and informed consent</span><p id="par0165" class="elsevierStylePara elsevierViewall">The authors have obtained the informed consent of the patients and&#47;or subjects referred to in this article&#46; The signed forms are in the possession of the corresponding author&#46;</p></span></span><span id="sec0085" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0145">Conflicts of Interest</span><p id="par0170" class="elsevierStylePara elsevierViewall">The authors declare that they have no conflicts of interest&#46;</p></span></span>"
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            0 => "Photodamage"
            1 => "Platelet-rich plasma"
            2 => "Growth factors"
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          "palabras" => array:3 [
            0 => "Fotoda&#241;o"
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        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Background</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Platelet-rich plasma &#40;PRP&#41; provides growth factors that stimulate fibroblast activation and induce the synthesis of collagen and other components of the extracellular matrix&#46; The objective of this study was to evaluate the effect of PRP in the treatment of photodamage of the skin of the hands&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Material and methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Experimental study enrolling persons with photoaged skin on the dorsum of the hands &#40;Glogau photoaging scale&#44; type III&#44; or Fitzpatrick wrinkle classification&#44; type II&#41; were included between August 2012 and January 2013&#46; A histological comparison was made of skin biopsies taken before and after the application of PRP to the skin of the dorsum of the hands&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">The mean &#40;SD&#41; age of the 18 women enrolled was 47&#46;9 &#40;4&#46;3&#41; years&#46; Histological analysis showed an increase in the number of fibroblasts &#40;<span class="elsevierStyleItalic">P</span>&#60;&#46;001&#41;&#44; number of vessels &#40;<span class="elsevierStyleItalic">P</span>&#60;&#46;001&#41;&#44; and collagen density &#40;<span class="elsevierStyleItalic">P</span>&#61;&#46;27&#41;&#46; These changes produced significant improvements in the Fitzpatrick wrinkle and elastosis scale &#40;<span class="elsevierStyleItalic">P</span>&#60;&#46;001&#41; and in the Glogau photoaging scale &#40;<span class="elsevierStyleItalic">P</span>&#61;&#46;01&#41;&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">PRP induced a reduction in the manifestations of skin aging&#44; including an improvement in wrinkles and elastosis&#46;</p></span>"
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        "resumen" => "<span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introducci&#243;n</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">El plasma rico en plaquetas &#40;PRP&#41; aporta factores de crecimiento que inducen la s&#237;ntesis de col&#225;geno y otros componentes de la matriz extracelular&#44; y estimula la activaci&#243;n de fibroblastos&#46; El objetivo de este estudio es evaluar el efecto del PRP en el tratamiento del fotoda&#241;o en manos&#46;</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Material y m&#233;todos</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Estudio de tipo experimental&#46; Se incluyeron individuos con envejecimiento en el dorso de las manos&#44; de tipo 3 en la escala de fotoda&#241;o de Glogau o de clase II de la clasificaci&#243;n de arrugas y elastosis de Fitzpatrick&#44; entre agosto de 2012 y enero de 2013&#46; Se aplic&#243; PRP en la piel del dorso de las manos y se tomaron biopsias cut&#225;neas antes y despu&#233;s del tratamiento para la comparaci&#243;n histol&#243;gica&#46;</p></span> <span id="abst0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Se trataron 18 mujeres con una edad media de 47&#44;9 <span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span> 4&#44;3 a&#241;os&#46; El an&#225;lisis histol&#243;gico demostr&#243; un incremento en el n&#250;mero de fibroblastos &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">0&#44;000</span>&#41;&#44; n&#250;mero de vasos &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">0&#44;000</span>&#41; y cantidad de col&#225;geno &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">&#61;</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">0&#44;27</span>&#41;&#44; representado como un cambio en la escala de clasificaci&#243;n de arrugas y elastosis de Fitzpatrick &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">0&#44;001</span>&#41; y en la escala de fotoda&#241;o de Glogau &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">0&#44;01</span>&#41;</p></span> <span id="abst0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Conclusiones</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">El PRP indujo una reducci&#243;n en los signos y s&#237;ntomas del envejecimiento cut&#225;neo&#44; con mejor&#237;a en las arrugas y elastosis&#46;</p></span>"
        "secciones" => array:4 [
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        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0010">Please cite this article as&#58; Cabrera-Ram&#237;rez JO&#44; Puebla-Mora AG&#44; Gonz&#225;lez-Ojeda A&#44; Garc&#237;a-Mart&#237;nez D&#44; Cort&#233;s-Lares JA&#44; M&#225;rquez-Vald&#233;s AR&#44; et al&#46; Plasma rico en plaquetas en el tratamiento del fotoda&#241;o cut&#225;neo en las manos&#46; Actas Dermosifiliogr&#46; 2017&#59;108&#58;746&#8211;751&#46;</p>"
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          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Dorsal surfaces of treated hands&#46; A&#44; Before treatments with platelet-rich plasma&#46; B&#44; After treatments&#46;</p>"
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          "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Histologic features of biopsied tissue&#59; hematoxylin and eosin&#44; original magnification &#215;40&#46; A&#44; After treatments with platelet-rich plasma the number of fibroblasts increased&#46; B&#44; Before treatments&#46;</p>"
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          "en" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Collagen fibers&#59; Masson&#39;s trichrome&#44; original magnification &#215;40&#46; A&#44; Before treatments with platelet-rich plasma&#46; B&#44; After treatments collagen had increased&#46;</p>"
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          "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">Histologic Findings Before and After Platelet-Rich Plasma Treatments&#46;</p>"
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      "titulo" => "References"
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              "etiqueta" => "1"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Fifty years of skin aging"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "M&#46; Yaar"
                            1 => "M&#46;S&#46; Eller"
                            2 => "B&#46;A&#46; Gilchrest"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "J Invest Dermatol Symposium Proceedings&#46;"
                        "fecha" => "2002"
                        "volumen" => "7"
                        "paginaInicial" => "51"
                        "paginaFinal" => "58"
                      ]
                    ]
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            1 => array:3 [
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              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Applications of platelet-rich fibrin matrix in facial plastic surgery"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "A&#46;P&#46; Sclafani"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1055/s-0029-1242033"
                      "Revista" => array:7 [
                        "tituloSerie" => "Facial Plast Surg&#46;"
                        "fecha" => "2009"
                        "volumen" => "25"
                        "paginaInicial" => "270"
                        "paginaFinal" => "276"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19924600"
                            "web" => "Medline"
                          ]
                        ]
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                          "pii" => "S0140673615003402"
                          "estado" => "S300"
                          "issn" => "01406736"
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                  ]
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              "identificador" => "bib0165"
              "etiqueta" => "3"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Can platelet-rich plasma be used for skin rejuvenation&#63; Evaluation of effects of platelet-rich plasma on human dermal fibroblast"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "D&#46;H&#46; Kim"
                            1 => "Y&#46;J&#46; Je"
                            2 => "C&#46;D&#46; Kim"
                            3 => "Y&#46;H&#46; Lee"
                            4 => "Y&#46;J&#46; Seo"
                            5 => "J&#46;H&#46; Lee"
                          ]
                        ]
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                    ]
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                    0 => array:2 [
                      "doi" => "10.5021/ad.2011.23.4.424"
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Dermatol&#46;"
                        "fecha" => "2011"
                        "volumen" => "23"
                        "paginaInicial" => "424"
                        "paginaFinal" => "431"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22148008"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
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              "etiqueta" => "4"
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                    0 => array:2 [
                      "titulo" => "The potential impact of the preparation rich in growth factors &#40;PRGF&#41; in different medical fields"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "E&#46; Anitua"
                            1 => "M&#46; Sanchez"
                            2 => "G&#46; Orive"
                            3 => "I&#46; And&#237;a"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.biomaterials.2007.06.037"
                      "Revista" => array:6 [
                        "tituloSerie" => "Biomaterials&#46;"
                        "fecha" => "2007"
                        "volumen" => "28"
                        "paginaInicial" => "4551"
                        "paginaFinal" => "4560"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17659771"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0175"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Systematic review of the use of platelet-rich plasma in aesthetic dermatology"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "M&#46;S&#46; Leo"
                            1 => "A&#46;S&#46; Kumar"
                            2 => "R&#46; Kirit"
                            3 => "R&#46; Konathan"
                            4 => "R&#46;K&#46; Sivamani"
                          ]
                        ]
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                    0 => array:2 [
                      "doi" => "10.1111/jocd.12167"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Cosmet Dermatol"
                        "fecha" => "2015"
                        "volumen" => "14"
                        "paginaInicial" => "315"
                        "paginaFinal" => "323"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26205133"
                            "web" => "Medline"
                          ]
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            5 => array:3 [
              "identificador" => "bib0180"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Benefits of plasma rich in growth factors &#40;PRGF&#41; in skin photodamage&#58; Clinical response and histological assessment"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "B&#46; D&#237;az-Ley"
                            1 => "J&#46; Cuevas"
                            2 => "L&#46; Alonso-Castro"
                            3 => "M&#46;I&#46; Calvo"
                            4 => "L&#46; R&#237;os-Buceta"
                            5 => "G&#46; Orive"
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                        ]
                      ]
                    ]
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                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/dth.12228"
                      "Revista" => array:7 [
                        "tituloSerie" => "Dermatol Ther&#46;"
                        "fecha" => "2015"
                        "volumen" => "28"
                        "paginaInicial" => "258"
                        "paginaFinal" => "263"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/25864463"
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                          ]
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                          "pii" => "S0735109713060294"
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                          "issn" => "07351097"
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              "identificador" => "bib0185"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:1 [
                  "referenciaCompleta" => "Online Archive of International cellular medicine society&#46; Section VIII Platelet rich plasma &#40;PRP&#41; guidelines 2011 &#91;cited 2014 Oct 3&#41;&#46; Available from&#58; <a href="http://www.cellmedicinesociety.org/attachments/370_Section%2010%20-%20Platelet%20Rich%20Plasma%20(PRP)%20Guidelines.pdf">http&#58;&#47;&#47;www&#46;cellmedicinesociety&#46;org&#47;attachments&#47;370&#95;Section&#37;2010&#37;20-&#37;20Platelet&#37;20Rich&#37;20Plasma&#37;20&#40;PRP&#41;&#37;20Guidelines&#46;pdf</a>&#46;"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The use of plasma-rich growth factors &#40;PRGF&#41; in oral surgery"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "E&#46; Anitua"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Pract Proced Aesthet Dent&#46;"
                        "fecha" => "2001"
                        "volumen" => "13"
                        "paginaInicial" => "487"
                        "paginaFinal" => "493"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11544821"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib0195"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Plasma rico en plaquetas&#59; una revisi&#243;n bibliogr&#225;fica"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "T&#46; Beca"
                            1 => "G&#46; Hern&#225;ndez"
                            2 => "S&#46; Morante"
                            3 => "A&#46; Bascones"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Av Periodon Implantol&#46;"
                        "fecha" => "2007"
                        "volumen" => "19"
                        "paginaInicial" => "39"
                        "paginaFinal" => "52"
                      ]
                    ]
                  ]
                ]
              ]
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            9 => array:3 [
              "identificador" => "bib0200"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The effects of autologous platelet gel on wound healing"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "J&#46; Henderson"
                            1 => "C&#46; Cupp"
                            2 => "E&#46; Ross"
                            3 => "P&#46; Shick"
                            4 => "M&#46; Keefe"
                            5 => "D&#46; Wester"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ear Nose Throat J&#46;"
                        "fecha" => "2003"
                        "volumen" => "82"
                        "paginaInicial" => "598"
                        "paginaFinal" => "602"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/14503096"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib0205"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Platelet-rich plasma combined with fractional laser therapy for skin rejuvenation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "M&#46; Shin"
                            1 => "J&#46; Lee"
                            2 => "S&#46; Lee"
                            3 => "N&#46; Kim"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/j.1524-4725.2011.02280.x"
                      "Revista" => array:7 [
                        "tituloSerie" => "Dermatol Surg&#46;"
                        "fecha" => "2012"
                        "volumen" => "38"
                        "paginaInicial" => "623"
                        "paginaFinal" => "630"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22288389"
                            "web" => "Medline"
                          ]
                        ]
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                          "pii" => "S0140673612617280"
                          "estado" => "S300"
                          "issn" => "01406736"
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                  ]
                ]
              ]
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            11 => array:3 [
              "identificador" => "bib0210"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Autologous platelet-rich plasma&#58; A potential therapeutic tool for promoting hair growth"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "Z&#46; Li"
                            1 => "H&#46; Choi"
                            2 => "D&#46; Choi"
                            3 => "K&#46; Sohn"
                            4 => "M&#46; Im"
                            5 => "Y&#46; Seo"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/j.1524-4725.2012.02394.x"
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                        "tituloSerie" => "Dermatol Surg&#46;"
                        "fecha" => "2012"
                        "volumen" => "38"
                        "paginaInicial" => "1040"
                        "paginaFinal" => "1046"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22455565"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib0215"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Photoaging&#44; mechanisms and repair"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "J&#46; Rabe"
                            1 => "A&#46; Mamelak"
                            2 => "P&#46; McElgunn"
                            3 => "W&#46; Morison"
                            4 => "D&#46; Sauder"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.jaad.2005.05.010"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Am Acad Dermatol&#46;"
                        "fecha" => "2006"
                        "volumen" => "55"
                        "paginaInicial" => "1"
                        "paginaFinal" => "13"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16781287"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
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            13 => array:3 [
              "identificador" => "bib0220"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Current applications of platelet gels in facial plastic surgery"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "S&#46; Bhanot"
                            1 => "J&#46;C&#46; Alex"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1055/s-2002-19824"
                      "Revista" => array:6 [
                        "tituloSerie" => "Facial Plast Surg&#46;"
                        "fecha" => "2002"
                        "volumen" => "18"
                        "paginaInicial" => "27"
                        "paginaFinal" => "33"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11823930"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib0225"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Gene therapy in wound repair and regeneration"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "E&#46; Yao"
                            1 => "E&#46; Eriksson"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Wound Repair Regen"
                        "fecha" => "2000"
                        "volumen" => "8"
                        "paginaInicial" => "4443"
                        "paginaFinal" => "4451"
                      ]
                    ]
                  ]
                ]
              ]
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            15 => array:3 [
              "identificador" => "bib0230"
              "etiqueta" => "16"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Terapia de bioestimulaci&#243;n con plasma rico en plaquetas para el envejecimiento cut&#225;neo"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "H&#46;M&#46; Escobar"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:3 [
                        "tituloSerie" => "Rev Argent Dermatol&#46;"
                        "fecha" => "2012"
                        "volumen" => "93"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            16 => array:3 [
              "identificador" => "bib0235"
              "etiqueta" => "17"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Histologic evidence of new collagen formulation using platelet rich plasma in skin rejuvenation&#58; A prospective controlled clinical study"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "O&#46;K&#46; Abuaf"
                            1 => "H&#46; Yildiz"
                            2 => "H&#46; Baloglu"
                            3 => "M&#46;E&#46; Bilgili"
                            4 => "H&#46;A&#46; Simsek"
                            5 => "B&#46; Dogan"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.5021/ad.2016.28.6.718"
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Dermatol"
                        "fecha" => "2016"
                        "volumen" => "28"
                        "paginaInicial" => "718"
                        "paginaFinal" => "724"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/27904271"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            17 => array:3 [
              "identificador" => "bib0240"
              "etiqueta" => "18"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Aesthetic and anatomic analysis of the aging skin"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "R&#46;G&#46; Glogau"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "Semin Cutan Med Surg&#46;"
                        "fecha" => "1996"
                        "volumen" => "15"
                        "paginaInicial" => "134"
                        "paginaFinal" => "138"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8948530"
                            "web" => "Medline"
                          ]
                        ]
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                          "pii" => "S0140673609603184"
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                          "issn" => "01406736"
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                  ]
                ]
              ]
            ]
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              "identificador" => "bib0245"
              "etiqueta" => "19"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pulsed carbon dioxide laser resurfacing of photo-aged facial skin"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "R&#46;E&#46; Fitzpatrick"
                            1 => "M&#46;P&#46; Goldman"
                            2 => "N&#46;M&#46; Satur"
                            3 => "W&#46;D&#46; Tope"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "Arch Dermatol"
                        "fecha" => "1996"
                        "volumen" => "132"
                        "paginaInicial" => "395"
                        "paginaFinal" => "402"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8629842"
                            "web" => "Medline"
                          ]
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                          "pii" => "S0140673613610971"
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                          "issn" => "01406736"
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                  ]
                ]
              ]
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              "identificador" => "bib0250"
              "etiqueta" => "20"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Relevant aspects of centrifugation step in the preparation of platelet-rich plasma"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "A&#46;G&#46; Perez"
                            1 => "J&#46;F&#46;S&#46; Lana"
                            2 => "A&#46;A&#46; Rodrigues"
                            3 => "A&#46;C&#46;M&#46; Luzo"
                            4 => "W&#46;D&#46; Belangero"
                            5 => "M&#46;H&#46;A&#46; Santana"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:2 [
                        "tituloSerie" => "ISRN Hematol"
                        "fecha" => "2014"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            20 => array:3 [
              "identificador" => "bib0255"
              "etiqueta" => "21"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lipoaugmentation for aging hands&#58; A comparision of the longevity and aesthetic results of centrifuged versus noncentrifuged fat"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "K&#46;J&#46; Butterwick"
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                    ]
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                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Dermatol Surg"
                        "fecha" => "2002"
                        "volumen" => "28"
                        "paginaInicial" => "987"
                        "paginaFinal" => "991"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12460290"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            21 => array:3 [
              "identificador" => "bib0260"
              "etiqueta" => "22"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Hand rejuvenation with structural fat grafting"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "S&#46;R&#46; Coleman"
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                    ]
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                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1097/01.PRS.0000033936.43357.08"
                      "Revista" => array:6 [
                        "tituloSerie" => "Plast Reconstr Surg"
                        "fecha" => "2002"
                        "volumen" => "110"
                        "paginaInicial" => "1731"
                        "paginaFinal" => "1744"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12447057"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            22 => array:3 [
              "identificador" => "bib0265"
              "etiqueta" => "23"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Proliferation-promoting effect of platelet-rich plasma on human adipose&#8211;derived stem cells and human dermal fibroblasts"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "N&#46; Kakudo"
                            1 => "T&#46; Minakata"
                            2 => "T&#46; Mitsui"
                            3 => "S&#46; Kushida"
                            4 => "F&#46;Z&#46; Notodihardjo"
                            5 => "K&#46; Kusumoto"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Plast Reconstr Surg&#46;"
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                        "volumen" => "11"
                        "paginaInicial" => "1352"
                        "paginaFinal" => "1360"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            23 => array:3 [
              "identificador" => "bib0270"
              "etiqueta" => "24"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Plasma rico en plaquetas &#191;Herramienta vers&#225;til de la medicina regenerativa&#63;"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "P&#46; Carrillo"
                            1 => "A&#46; Gonzalez"
                            2 => "S&#46; Macias"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Cir Cir&#46;"
                        "fecha" => "2013"
                        "volumen" => "81"
                        "paginaInicial" => "74"
                        "paginaFinal" => "82"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23461926"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
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              "identificador" => "bib0275"
              "etiqueta" => "25"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Assessment of the efficacy and safety of single platelet-rich plasma injection on different types and grades of facial wrinkles"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "N&#46;Y&#46; Elnehrawy"
                            1 => "Z&#46;A&#46; Ibrahim"
                            2 => "A&#46;M&#46; Eltoukhy"
                            3 => "H&#46;M&#46; Nagy"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/jocd.12258"
                      "Revista" => array:2 [
                        "tituloSerie" => "J Cosmet Dermatol"
                        "fecha" => "2016"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            25 => array:3 [
              "identificador" => "bib0280"
              "etiqueta" => "26"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Platelet-rich plasma&#58; Support for its use in wound healing"
                      "autores" => array:1 [
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                          "etal" => false
                          "autores" => array:2 [
                            0 => "K&#46; Lacci"
                            1 => "A&#46; Dardik"
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                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "Yale J Biol Med&#46;"
                        "fecha" => "2010"
                        "volumen" => "83"
                        "paginaInicial" => "1"
                        "paginaFinal" => "9"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20351977"
                            "web" => "Medline"
                          ]
                        ]
                        "itemHostRev" => array:3 [
                          "pii" => "S0735109714068363"
                          "estado" => "S300"
                          "issn" => "07351097"
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                  ]
                ]
              ]
            ]
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              "identificador" => "bib0285"
              "etiqueta" => "27"
              "referencia" => array:1 [
                0 => array:2 [
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                    0 => array:2 [
                      "titulo" => "Plasma rico en plaquetas en cirug&#237;a est&#233;tica"
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                        "paginaInicial" => "25e34"
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                    ]
                  ]
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                      "titulo" => "Improvement in the appearance of wrinkles with topical transforming growth factor beta and 1-ascorbic acid"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "M&#46; Ehrlich"
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                      ]
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                        "tituloSerie" => "Dermatol Surg"
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                      "titulo" => "Efficacy of novel skin cream containing mixture of human growth factors"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "M&#46;H&#46; Gold"
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                        ]
                      ]
                    ]
                  ]
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                      "Revista" => array:6 [
                        "tituloSerie" => "J Drugs Dermatol&#46;"
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                        "paginaFinal" => "201"
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Article information

ISSN: 15782190
Original language: English

DOI:10.1016/j.adengl.2017.07.002

The statistics are updated each day

Year/Month	Html	Pdf	Total

2024 November	11	16	27
2024 October	81	40	121
2024 September	101	44	145
2024 August	130	84	214
2024 July	109	65	174
2024 June	97	57	154
2024 May	66	43	109
2024 April	80	36	116
2024 March	98	33	131
2024 February	87	38	125
2024 January	70	34	104
2023 December	67	16	83
2023 November	94	33	127
2023 October	86	37	123
2023 September	82	41	123
2023 August	52	25	77
2023 July	60	41	101
2023 June	79	29	108
2023 May	94	29	123
2023 April	59	24	83
2023 March	69	37	106
2023 February	80	28	108
2023 January	50	36	86
2022 December	76	48	124
2022 November	53	30	83
2022 October	33	36	69
2022 September	39	62	101
2022 August	29	40	69
2022 July	44	44	88
2022 June	31	28	59
2022 May	32	61	93
2022 April	59	38	97
2022 March	64	47	111
2022 February	35	32	67
2022 January	42	41	83
2021 December	36	40	76
2021 November	55	74	129
2021 October	53	76	129
2021 September	48	57	105
2021 August	44	48	92
2021 July	49	37	86
2021 June	43	41	84
2021 May	64	64	128
2021 April	83	84	167
2021 March	80	60	140
2021 February	65	38	103
2021 January	61	40	101
2020 December	67	43	110
2020 November	62	39	101
2020 October	30	28	58
2020 September	58	19	77
2020 August	37	23	60
2020 July	42	30	72
2020 June	63	36	99
2020 May	20	13	33
2020 April	38	16	54
2020 March	40	18	58
2020 February	5	0	5
2020 January	4	0	4
2019 December	8	0	8
2019 November	4	0	4
2019 September	8	0	8
2019 August	2	0	2
2019 July	6	0	6
2019 June	6	0	6
2019 May	2	0	2
2019 April	4	0	4
2019 March	4	0	4
2019 January	3	0	3
2018 December	2	0	2
2018 November	2	0	2
2018 October	3	0	3
2018 September	6	0	6
2018 February	30	12	42
2018 January	58	14	72
2017 December	57	32	89
2017 November	63	28	91
2017 October	142	71	213
2017 September	5	26	31
2017 August	5	22	27

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