Article information
Abstract
Background
Cutaneous lymphocyte antigen (CLA) is expressed by a subgroup of memory T cells that exhibit skin homing and are implicated in cutaneous T-cell-mediated diseases.
Material and methodsExpression of genes associated with psoriasis was analyzed in keratinocytes taken from patients and healthy individuals and cultured under different conditions, including activation using supernatants from CLA+ T lymphocytes activated with anti-CD3 and anti-CD28 antibodies.
ResultsKeratinocytes from psoriasis patients activated by CLA+ T lymphocytes expressed higher levels of interferon-inducible protein 10, HLA-DR, intercellular cell adhesion molecule 1, and inducible nitric oxide synthase.
ConclusionsOur results suggest that we have developed an in vitro model that will allow analysis of the effector role of CLA+ T lymphocytes on keratinocytes in psoriasis. This model may allow the identification of genes involved in the pathology of psoriasis through induction by CLA+ T lymphocytes.
Key words:
psoriasis
cutaneous lymphocyte antigen
keratinocyte
inducible nitric oxide synthase
Resumen
Introducción
Los linfocitos T CLA+ representan un subgrupo de linfocitos T de memoria con tropismo cutáneo que están implicados en diferentes enfermedades cutáneas mediadas por células T.
Material y métodosSe estudió la expresión de algunos genes asociados a la psoriasis en queratinocitos de enfermos y sanos cultivados en diferentes condiciones, entre ellas la activación por sobrenadantes de linfocitos T CLA+ activados mediante anti-CD3/anti-CD28.
ResultadosLos queratinocitos psoriásicos activados por linfocitos CLA+ expresan más IP-10, HLA-DR, ICAM-1 e iNOS.
DiscusiónEstos resultados sugieren que hemos establecido un modelo in vitro que permite estudiar la función efectora de los linfocitos T CLA+ sobre los queratinocitos en la psoriasis. Este modelo puede permitir la identificación de genes relevantes en la patogenia de la psoriasis al ser inducidos por linfocitos T CLA+.
Palabras clave:
psoriasis
antígeno linfocitario cutáneo
CLA
queratinocito
iNOS
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Study financed by an award from Fundation Salud 2000 for study of clinical immunology of psoriasis (2003).
Copyright © 2008. Academia Española de Dermatología y Venereología and Elsevier España, S.L.
