The Beginnings of Dermatopathology and Dermatologic Microbiology in Spain

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"apellidos" => "del Río" "email" => array:1 [ 0 => "dermalar@clinicadermalar.com" ] ] ] "afiliaciones" => array:1 [ 0 => array:2 [ "entidad" => "Clínica Dermalar, Santiago de Compostela, Spain" "identificador" => "aff0005" ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Los comienzos de la Dermatopatología y de la Microbiología dermatológica en España" ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0065" "etiqueta" => "Figure 13" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr13.jpeg" "Alto" => 2586 "Ancho" => 1583 "Tamanyo" => 810048 ] ] "descripcion" => array:1 [ "en" => "Page with figures in the monograph on pseudoepithelioma by Juan de Azúa and Claudio Sala Pons, illustrating this publication's most important contribution: pseudoepitheliomatous hyperplasia." ] ] ] "textoCompleto" => "IntroductionMedicine is multidisciplinary by nature, but the characteristics of each discipline's store of knowledge and its peculiar requirements mean that some branches have more in common than others. Dermatology is especially dependent on microbiology and above all on pathology, to the extent that we remain unable to conceive of good clinical practice in our specialty without their support. This is the case even as our need for knowledge of genetics and immunology becomes as great or greater. By the second half of the 19th century, both microbiology and pathology had already taken their familiar forms. Their practices developed somewhat later than those of dermatology itself, whose origins can be traced to the beginning of the same century.Thus, the 3 specialties emerged and developed in Spain almost simultaneously. All were in evidence in the second half of the 19th century and were well established by the first third of the 20th. In fact, dermatology—through the pioneering laboratory of Hospital de San Juan de Dios in Madrid—provided essential support for the advancement of histopathology and microbiology.This article reviews the fundamental principles, history, and interrelations that brought pathology, microbiology, and dermatology together in Spain, covering the early period of emergence and consolidation in the second half of the 19th century and their coming of age in the first half of the 20th. As the story has been little studied and microbiologists, pathologists, and dermatologists are not fully aware of it, this record represents a modest effort to pay homage to the historical contribution of the laboratory technicians whose work provided the background for clinical practice.Sources of Information on Dermatology and the Specialty's Relation to Microbiology and PathologyVery few studies have explored the relations among the 3 disciplines under consideration here. Ten years ago, Félix Contreras Rubio<a class="elsevierStyleCrossRef" href="#bib0005">1</a> published a brief overview of the recent history of dermatopathology in Spain, and Julio Bassas-Vila<a class="elsevierStyleCrossRef" href="#bib0010">2</a> has also provided general reflections on the subject.A key secondary source of information about the early period of Spanish pathology is the doctoral thesis of Roberto Marco Cuéllar,<a class="elsevierStyleCrossRef" href="#bib0015">3</a> written under the supervision of Professor José María López Piñero in Valencia and completed in 1966. This excellent thesis was rigorous and almost encyclopedic in its scope, as it delved into the emergence and early development of pathology before Ramón y Cajal. Surprisingly, it has never been published, although Marco Cuéllar took some material from his thesis for a chapter on histology and cytology in the fifth volume of the monumental history of medicine edited by Pedro Laín Entralgo (Historia Universal de la Medicina). After receiving degrees in both chemistry and medicine, Marco Cuéllar went on to earn his PhD in medicine. He then began a career in biochemistry and in 1984 became full professor of that subject at the Universidad Autónoma de Madrid. His career pathway perhaps explains the limited public dissemination of his thesis on the history of medicine. Marco Cuéllar died on June 27, 2008.<a class="elsevierStyleCrossRef" href="#bib0020">4</a>The history of microbiology in Spain has been thoroughly studied by María José Báguena Cervellera,<a class="elsevierStyleCrossRef" href="#bib0025">5</a> whose thesis on that subject was also supervised by López Piñero. This thesis likewise remains unpublished, even though it is rigorous and the amount of information it holds makes it worthy of wider circulation and publication.Finally, the present author's doctoral thesis on the history of dermatology,<a class="elsevierStyleCrossRef" href="#bib0030">6</a> completed in 1996 under the supervision of Professor Antonio García Pérez, covered the origins of the school of dermatologic theory and practice in Madrid, the first to appear in this field in Spain. This thesis was based on a considerable number of primary sources and original documents.The Early Years of Microscopy: Crisóstomo Martínez, the First of the Spanish MicroscopistsEarly microscopes are said to have been mainly used to entertain rather than to serve medical purposes. Microscopes were available as early as the first decades of the 17th century, but it was the curiosity of a Dutch merchant, Antonie van Leeuwenhoek, that led him to polish lenses and design instruments capable of magnification ×240, thus opening up a view of a previously unimagined world and making real progress possible. The first to see ciliates and other protozoa in water, van Leeuwenhoek called them animalcules and gave a full account of his observations in letters to the Royal Society of London; he also saw sperm cells, although he did not form a clear notion of their significance, and he may have seen red blood cells and bacteria. Nearly 2 centuries would have to pass, however, before this knowledge would be applied in medicine.Robert Hooke, who was nearly a contemporary of van Leeuwenhoek, authored an interesting treatise titled Micrographia, published in 1665. Another noteworthy 17th-century user of microscopes was the Italian Marcelo Malpighi. His name is familiar to us today because of an eponymous term: the Malpighian layer of the skin, referring to the nucleated basal, spinous, and granular strata of the epidermis, excluding the stratum corneum.Among the first Spaniards to use the new instrument was Crisóstomo Martínez (1638–1694), who was a member of the first generation of microscopists that included van Leeuwenhoek and Hooke. Martínez left a beautiful collection of engravings showing the structure of bone. In a still life decorating the edge of one of the anatomical illustrations, there is a single-lens microscope (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>). This detail was used on the front cover of a monograph on the history of microscopic anatomy in 17th- and 18th-century Spain by María Luz Terrada Ferrandis.<a class="elsevierStyleCrossRef" href="#bib0035">7</a><elsevierMultimedia ident="fig0005"></elsevierMultimedia>Earliest Microscopic Images of the Skin in the Spanish Medical LiteratureMartín Martínez, a celebrated Spanish physician at the end of the 17th century and beginning of the 18th century, was responsible for what were probably the first illustrations of the skin as seen under a microscope to be published in Spain. The 2 small illustrations appeared in a 1728 human anatomy book, inserted into the upper corners of a plate. The one depicting a reticulated pattern in the upper right corner (<a class="elsevierStyleCrossRef" href="#fig0010">Fig. 2</a>) was described thus by Martínez as showing “a portion of the skin with its epidermis.” The other drawing (<a class="elsevierStyleCrossRef" href="#fig0015">Fig. 3</a>), which was also given the number 3 in the source text, was said to represent “the manufacture of the skin, as revealed under the microscope: A. Cutaneous glands. B. Nerve papillae. C. Pores. D. Hairs. E. Subcutaneous vessels.” Exaggerated bumps appear on the surface of the skin and the hairs have very unrealistic branches; clearly this drawing reflects either the microscopist's misinterpretation or the engraver's artistic license.<elsevierMultimedia ident="fig0010"></elsevierMultimedia><elsevierMultimedia ident="fig0015"></elsevierMultimedia>Over the course of the 19th century, particularly between 1825 and 1875, the microscopic structures of the skin gradually became familiar. By the last quarter of the century, Spanish dermatology texts were being published with plates depicting cutaneous structures in detail. One of the earliest was Olavide's monumental general dermatology textbook published in 1871. Several plates show histologic structures of the skin and adnexa. Interesting illustrations of histologic sections (<a class="elsevierStyleCrossRef" href="#fig0020">Fig. 4</a>) can also be found in a somewhat forgotten and less costly alternative to Olavide's much larger and more attractive work. This was the clinical manual (álbum) on dermatology by Jerónimo Pérez Ortiz,<a class="elsevierStyleCrossRef" href="#bib0040">8</a> published in 1886. Neither author seems to have prepared the histologic sections that illustrate their work, however. Rather, they seem to have reproduced illustrations and borrowed information from books already in print elsewhere in Europe.<elsevierMultimedia ident="fig0020"></elsevierMultimedia>Improvements in Microscopes and Staining Methods in the 19th CenturyThe methods used in microscopy advanced relatively little in the 18th century. Instruments were simple single-lens devices that could reveal little more than van Leeuwenhoek had managed to see. Furthermore, both these simple devices and the early compound microscopes distorted shapes and colors. After 1820 these problems began to disappear with the arrival of new lenses manufactured and polished mainly in England. Pioneers in the development of compound microscopes free of optical distortions were Carl Zeiss, in Germany, and Camille Sebastien Nachet, in France. The instruments collected by Professor Eliseo Carrascal at the University of Salamanca admirably trace the evolution of microscopes and the variations in their design. Several Internet sites display photographs of microscopes from this period and explain their characteristics.Marco Cuéllar<a class="elsevierStyleCrossRef" href="#bib0015">3</a> mentions a book by Antonio Mendoza y Rueda, published in Barcelona in 1850. Though the author's title referred to clinical studies in surgery,<a class="elsevierStyleCrossRef" href="#bib0045">9</a> the second part of the volume had a large section on the microscope and its most important practical applications. Lesson 19 covered both simple and compound microscopes and their main accessories, such as diaphragms, condensers, the camera obscura and the camera lucida (or camera clara), prisms, etc. Mendoza y Rueda also discussed how objects are prepared for viewing—using the methods of Purkinje, Hannover, Jacobson and Raspail—as well as the double-bladed knife developed by Valentin for cutting thin sections.Another key advance was the move away from reflected light toward better systems of specimen illumination, which compound microscopes required. The middle quarters of the 19th century witnessed great improvements in microtomes, illumination, and staining methods.Specimen dissection was the subject of a book by José María Gómez Alamá (Arte de disecar). Its second edition (1872) included important references to different types of single-lens and compound microscopes. Included were solar microscopes and models incorporating early arc lamps. He also discussed the various types of micrometers (some with threaded screws, others with scales etched into glass disks), and set out guidelines for achieving adequate illumination of objects by reflection or transmission. Gómez Alamá named 2 conditions to meet when preparing tissue samples: the section must be extremely thin and the surfaces must be perfectly flat. To help readers achieve both requirements, he gave advice on managing scalpels, fine saws, and polishing stones for bones or minerals. Marco Cuéllar<a class="elsevierStyleCrossRef" href="#bib0015">3</a> reports that he mentioned an early version of a microtome by Straus-Durchen (sic.), a reference to Strauss-Dürckheim. The book contains illustrations of various instruments used at the time (<a class="elsevierStyleCrossRef" href="#fig0025">Fig. 5</a>).<elsevierMultimedia ident="fig0025"></elsevierMultimedia>This same period saw the introduction of a well-known staining technique for classifying bacteria into 2 broad groups. The system is the same one we use today and was proposed in 1884 by Hans Christian Gram of Denmark.<a class="elsevierStyleCrossRef" href="#bib0050">10</a>Early methods for working with microscopes grew out of a great deal of experimentation and required skilled craftsmanship. One of the most interesting accounts of the process of staining samples for microscopy at the end of the 19th century was written in the field of dermatology, specifically the doctoral thesis on Achorion schoenleinii and tinea favosa, by José Olavide Malo,<a class="elsevierStyleCrossRef" href="#bib0055">11</a> the son of José Eugenio de Olavide Landazába. The thesis, which was published in 1888, gave a detailed description of how fungal samples were prepared. First the material was placed in an antiseptic solution (mercury chloride at a concentration of 1 part per 1000) for 2minutes. It was then inserted into a tube with gelatin, cooled to 40°C and cooled further under running water until the gel had solidified, and finally incubated at a temperature of 27°C to 28°C. Fungal colonies appeared in 6 to 8 days and could then be studied under a microscope. Olavide Malo proposed 5 steps, and they are quoted here for the historical interest of the description:“1st, the organic matter is softened in an alkaline solution, preferably one containing potassium; 2nd, oils are removed by treating the fungus with alcohol followed by sulfuric ether or chloroform or any other solvent of oils; 3rd, portions of the vegetable material is stained: one should choose potassium, to give a blue color, or eosin in water, or Bismarck brown...; 4th, the vegetable material is then treated with a very strong solution (equal parts) of potassium acetate and distilled water in order to dissolve the excess color taken up by the bulk of the primitive stem so that the tissue is more transparent; and 5th, the sample is finally fixed on a slide: instead of Canada balsam, use a thick gum in solution, adding a few drops of phenic acid to prevent the fungi from escaping into the air.”Olavide Malo went on to explain how he undertook mycologic studies by growing the samples to increase the volume and embedding the material in paraffin (as well as in gelatin and in soap, although these media were judged inferior to paraffin) for later slicing with the freezing microtome introduced by Thomas Taylor or the rocking microtome manufactured by the Cambridge Instrument Company. Of the second microtome, the author says, “It is very appropriate for this type of work, providing an excellent series of slices with paraffin edges that line up squarely as they leave the microtome on a conveyor belt some 50 or 60cm long.”A year before Olavide Malo presented his thesis to the tribunal, Julio Magraner Marinas<a class="elsevierStyleCrossRef" href="#bib0025">5</a> had already written the following summary of a quarter of a century of work in this field:“Work done with microscopes over the past 25 years has revealed that animals contain within themselves numerous colonies of living organisms; although many of these organisms are harmless, some are closely joined to the existence and production of various diseases. It was a question of experimenting, and now our experiments have answered in the affirmative.”Within a few years, he would be proven right.Microscopy Arrives in 19th-Century Spain by Way of ParisMost of our knowledge of histology and pathology dates from the period between 1825 and 1848. The main contributors were German scientists like Henle, Purkinje, Müller, Schwann, and Schleiden. Soon after this period, the circle that formed around Rudolf Virchow would play a key role. However, modern microscopic methods traveled to 19th-century Spain by way of Paris. Interestingly, a Venezuelan microscopist—Eloy Carlos Ordóñez Sordo, whom most sources refer to simply as Ordóñez—was closely linked to the development of techniques in Spain. Ordóñez Sordo was born in 1822 in Girón, a village in the province of Pamplona in Nueva Granada, one of the 3 republics of the old state of Gran Colombia. His parents were from Maracaibo, Venezuela, and he studied in Santa Fe de Bogotá. After graduation, he accompanied Archbishop Mosquera on a journey from Colombia to New York, where he stayed for 2 years. On the archbishop's death, Ordóñez Sordo settled in Paris and devoted himself to the study of microscopy, probably from 1851 onward.<a class="elsevierStyleCrossRef" href="#bib0015">3</a> He died on May 19, 1868, at the age of 46 years.In discussing dermatopathology, Marco Cuéllar<a class="elsevierStyleCrossRef" href="#bib0015">3</a> cites the 1858 French publications of Ordóñez Sordo: a report describing a pigmented tumor—probably a melanoma—in the Bulletin de la Société d’Anatomie; a report to the Society of Biologists in France on an indurated chancre on the foreskin which was reviewed in the Spanish bulletins El Siglo Médico and El Compilador Médico; and another 1863 report on hair muscles written for the same biology association.Ordoñez Sordo proposed a system for classifying tissues into 16 groups, which Aureliano Maestre de San Juan included in his book on general anatomy. Marco Cuéllar<a class="elsevierStyleCrossRef" href="#bib0015">3</a> lists them as fibrillar (cellular, laminar, or conjunctival), fibrous (transient, fibro-plastic), elastic, adipose, cartilaginous, osseous, animal muscular (striated muscle), organic muscular (smooth muscle), epithelial, hyaline or anhistic, pigmented (lamina fusca, choroid, skin), retinal, nervous, enamel (of teeth), ivory (also of teeth), and crystalline.Two men who studied under Ordóñez Sordo—Aureliano Maestre de San Juan and Federico Rubio Galí—established schools of practice in the last quarter of the 19th century. The first was associated largely with university laboratories and was the result of Maestre de San Juan's interest in the pure study of morphology and histology, in keeping with his training as an anatomist. The second reflected Rubio Galí’s more clinical inclinations. His work involved a less constrained style of extramural education and leaned more clearly in the direction of pathology.Aureliano Maestre de San JuanAn eminent anatomist, Maestre de San Juan (<a class="elsevierStyleCrossRef" href="#fig0030">Fig. 6</a>) established a solid foundation for histology in Spain, according to the account of Marco Cuéllar.<a class="elsevierStyleCrossRef" href="#bib0015">3</a> He largely renounced research that could lead to original contributions in order to carry out the more mundane task of building up a store of information. Thus, he saw himself more as a laboratory technician than a scholar, and he shared his knowledge with his contemporaries. Born in Granada on October 17, 1828, he took his degree in the first stage of medical studies (bachillerato) in the same city and then spent 3 years completing his medical education in Madrid, where he graduated on November 8, 1847, at the age of 19 years. He received his doctorate in Madrid in 1851 and returned to Granada in 1856 to work as a clinical professor in the university's faculty of medicine. In 1860 he competed for a professorship in general anatomy in Granada and occupied that position until he returned to Madrid in 1873 to take up a full professorship in normal and pathologic histology. A laboratory accident caused chemical burns that cost him his sight in 1888, and he died in Alicante on June 1, 1890. Maestre de San Juan lived simply, spending most of his money on books and materials for his work with the microscope.<elsevierMultimedia ident="fig0030"></elsevierMultimedia>One of this anatomist's most important contributions was to found the Histologic Society on February 22, 1874, in the company of Andrés del Busto, Miguel Colmeiro, Gabriel de la Puerta, and the Venezuelan Francisco José Delgado Jugo, among others.<a class="elsevierStyleCrossRef" href="#bib0015">3</a> None of these men were engaged with the fields of dermatopathology or skin microbiology, probably because they felt no need to confine themselves to such specific areas at this time. The society they founded was short-lived—it disappeared after 3 years—but the section on histology of the Academy of Physicians and Surgeons of Madrid carried the project forward. The formation of the Histologic Society was thus an important step toward laying a solid foundation for microscopic research in Spain.Marco Cuéllar<a class="elsevierStyleCrossRef" href="#bib0015">3</a> reports that in 1879 Maestre de San Juan published a treatise on normal and pathologic histology that included a discussion of laboratory technique (Tratado elemental de Histología normal y patológica precedido de un resumen de técnica de laboratorio). A second, extensively revised edition published in 1885 dealt with the compound microscope in greater depth and gave a good discussion of the Natchet (<a class="elsevierStyleCrossRef" href="#fig0035">Fig. 7</a>) and Verick microscopes, the camera lucida, micrometers, polarization devices, goniometers, immersion lenses, correction, heated slides and other specimen holders, rotating components, mounts for dissection, prism inverters, the Abbe condenser, and the spectroscope. A section on chemicals, vehicles, and reagents covered harmless vehicles (water, physiologic serum, amniotic fluid), reagents (alcohol and acetic, chromic, picric and other acids), preserving agents (glycerin, turpentine), cements (bitumen of Judea, Zeigler's bitumen), stains that impregnate tissues (silver nitrate, gold or palladium chloride, osmic acid), and stains that act by inhibition (carmine, picrocarminate, Ranvier purpurin, sulfate, and rosaniline acetate, fuchsine or aniline red, quinoline blue, hematoxylin, indigo carmine, eosin, and more). Also discussed were preserving and clearing agents such as glycerin, turpentine, Canada balsam, clove oil, and dammar resin.<a class="elsevierStyleCrossRef" href="#bib0015">3</a><elsevierMultimedia ident="fig0035"></elsevierMultimedia>Although Maestre de San Juan initially hesitated to align himself with a particular approach, he finally opted to follow Rudolf Virchow's German school of histology, while still conscious of the debt he owed his French training. He used, for example, a Ranvier microtome, which was little more than a screw and shaving razor. Methods for fixing samples were being introduced at this time. Initially, gum arabic was used. Freezing was also discussed, although it was felt to be difficult to find equipment that could achieve the desired result.Federico Rubio GalíAnother key figure, was Rubio Galí (<a class="elsevierStyleCrossRef" href="#fig0040">Fig. 8</a>), a histologist and surgeon whose interest in the microscope dated from the time he spent working in Seville. There, he joined a group of surgeons who used histologic techniques themselves or took an interest in them. Others in this circle, Marco Cuéllar<a class="elsevierStyleCrossRef" href="#bib0015">3</a> reports, were Adolfo Moreno Pozo, Juan Creus Manso, Santiago González Encinas, and José Calvo Martín.<elsevierMultimedia ident="fig0040"></elsevierMultimedia>Rubio Galí was born in Puerto de Santa María in 1827 and studied medicine in Cadiz, graduating in 1850 with high marks. He won a post at the Hospital Central de Sevilla that same year, but his political liberalism led to serious professional setbacks. At one point he had to give classes in fencing—a skill he excelled at—in order to make ends meet. His interest in histology began during a period of exile to France, where he became interested in Ordóñez Sordo, according to Marco Cuéllar.<a class="elsevierStyleCrossRef" href="#bib0015">3</a> He returned to Seville for a time and later worked in Madrid.In 1872 he authored a paper on an analysis of air in the hospital wards of Dr Ezequiel Martín de Pedro in the Hospital General de Madrid<a class="elsevierStyleCrossRef" href="#bib0060">12</a>; the analysis was accomplished in collaboration with José Eugenio de Olavide. They found various bacteria and described 3 types: bacterium punctum (which we would refer to as cocci today), bacterium catenula (chains of 2—i.e., diplococci—or 3 cocci), and bacterium baculum (bacilli). The publication even described how these bacteria moved.Most of the histologic preparations for the autopsies Olavide later described in his anatomic atlas were done by Rubio Galí. Some of these preparations, even made it possible to supplement the color plates of the atlas with black and white illustrations of clinical lesions (<a class="elsevierStyleCrossRefs" href="#fig0045">Figs. 9 and 10</a>).<elsevierMultimedia ident="fig0045"></elsevierMultimedia><elsevierMultimedia ident="fig0050"></elsevierMultimedia>Salvador CardenalBorn in Valencia in 1852, Salvador Cardenal studied to be a surgeon in Barcelona, graduating in 1875. Cardenal's report on histologic and clinical characteristics of lupus, epithelioma, and ulcerating tumors, which also compared their different treatments, won a competition organized by the Royal Academy of Medicine in Madrid. Marco Cuéllar<a class="elsevierStyleCrossRef" href="#bib0015">3</a> notes that the report revealed Cardenal's considerable understanding of histologic techniques and a wide range of stains and fixing agents. Cardenal recommended carminic acid with the addition of picric acid to stain epithelioma samples, for example. He also published a manual on surgical antisepsis, of which several editions were printed.Cardenal's work gave evidence of the many advances that led to enormous growth in knowledge in the field of microbiology in the last third of the 19th century, according to Báguena Cervellera.<a class="elsevierStyleCrossRef" href="#bib0025">5</a> Relevant advances included improvements in the manufacture of microscopes (better objectives and apochromatic light-condensing lenses) and the use of aniline dyes to stain microorganisms.Some Contributions of Spanish Microbiology to EpidemiologyThe study of infectious diseases was an independently progressing field that furthered the development of microscopy, as presented by Báguena Cervellera<a class="elsevierStyleCrossRef" href="#bib0025">5</a> in her thesis. Pablo Colvée, for example, demonstrated the means of contagion during an epidemic of trichinosis in the town of Villar del Arzobispo in 1877. Vicente Peset Cervera, who had studied both medicine and chemistry, published on the role of fermentation in physiology and pathology in 1878. His meticulous studies of the fermentation of foods in the digestive tract followed a line similar to Pasteur's. From this work it was possible to infer the existence of microorganisms that promoted the digestive process, ultimately undermining the theory of spontaneous generation.Báguena Cervellera<a class="elsevierStyleCrossRef" href="#bib0025">5</a> also describes an 1898 textbook on basic microbiology for students of human and veterinary medicine by Luis del Río y Lara, professor of histology at the university in Saragossa. Del Río y Lara's book was the first to present microbiology as a discipline separate from pathology. A considerable number of pages in this book were devoted to 3 diseases relevant to dermatology: actinomycosis, tuberculosis in its various forms, and leprosy. Cocci (staphylococci, streptococci, tetrads, and sarcinae) were mentioned in relation to phlegmon- and abscess-causing pyodermatitis. Del Río y Lara also studied lymphangitis, erysipelas (which he referred to as streptococcal dermatitis of Fehleisen) and carbuncles. Syphilis, trypanosomiasis (durina), and gonococcal infection were also given ample treatment. Del Río y Lara customarily used a glycerinated gel-forming broth that already contained agar-agar and resembled the media used in microbiology today.José Eugenio de Olavide published a short paper on Vibrio cholerae<a class="elsevierStyleCrossRef" href="#bib0065">13</a> in 1884, the same year Robert Koch described this bacterium. This publication apparently bore no relation to his practice of dermatology. He was working at the laboratory of Hospital San Juan de Dios, which belonged to the provincial government of Madrid (the Diputación). This hospital was a center of reference in the effort to control the cholera epidemics raging in Spain at the end of the 19th century.Plant and Animal ParasitismDermatology played an important role in helping the concept of contagion take root in medicine because lice infestation and scabies provided the paradigm for parasitic diseases in general and for disease transmission. It was reasoned that if lice and mites could pass from one individual to another and cause disease, even smaller parasites that were as yet unidentified could also do so.José Eugenio de Olavide's atlas of anatomy contained a magnificent illustration of the Sarcoptes scabiei mite and explained the transmission of scabies.Báguena Cervellera<a class="elsevierStyleCrossRef" href="#bib0025">5</a> also notes the gradual acceptance of the idea that plants—fungi to be precise—could sometimes cause disease after Agostino Bassi demonstrated, around 1830, that a fungus was responsible for muscadine in silk worms. Just such a fungal disease provides the starting point for the story in Alessandro Baricco's recent successful novel, Silk.The first studies of parasitic plants were done by botanists. Johann Schönlein, Jacob Henle, and Ernest Bazin were outstanding contributors to this branch of knowledge in the middle decades of the 19th century; other names associated with parasitic plant studies included Gruby, Richter, Audouin, Vogel, and Lebert.<a class="elsevierStyleCrossRef" href="#bib0070">14</a> In the previously mentioned monograph on cholera by Olavide,<a class="elsevierStyleCrossRef" href="#bib0065">13</a> it is interesting that he referred to the bacterial pathogen as a “microscopic plant.” The influence of botany on microbiology may surprise us today, but even now our language reflects the ties between these disciplines, as we continue to refer to microorganisms in general as germs.Historically Important Research FacilitiesLaboratories were—and continue to be—the places where microbiologists and dermatopathologists conduct their research. Therefore, it is essential to name the important research facilities as well as the relevant scientists. Báguena Cervellera<a class="elsevierStyleCrossRef" href="#bib0025">5</a> described them in her thesis. One was the outstanding laboratory for the study of normal histology, pathology, and bacteriology founded in 1875 by Maestre de San Juan at the faculty of medicine in Madrid. Another important laboratory in the same city was the National Institute of Hygiene and Bacteriology, established in 1894. This facility would grow into the larger National Institute of Serotherapy, Vaccination and Bacteriology (also called the Alfonso XIII National Institute of Hygiene), which was created by Royal Decree on October 28, 1899. The laboratory at Madrid's military hospital—where Rafael Ariza and José Alabern worked—must also be mentioned. That facility undertook both chemical and microscopic studies. Finally, the laboratory of Hospital de San Juan de Dios mentioned earlier will merit special attention in a separate section below.The city of Barcelona created its own municipal laboratory in 1887. Also noteworthy in the same city were the Ferrán Institute (where Jaime Ferrán did his work) and the Institute of Biology (where Ramón Turró worked).<a class="elsevierStyleCrossRef" href="#bib0025">5</a>Microscopy and Histochemistry: Microbiology, Pathology, and Clinical BiochemistryGiven our current notion of microbiology, pathology, and clinical biochemistry as distinct disciplines, it seems striking to us today that the early microscopists worked simultaneously in all these branches of knowledge. Microbiology and pathology were thrown together for a simple reason: they used the same tool—the microscope—as well as similar chemicals for analysis and staining. The term microscopy (or the contemporary alternative micrography) originally encompassed both those disciplines and was widely used inclusively to refer to them at the end of the 19th century. The link to clinical biochemistry may seem more tenuous, but in fact this field was once called histochemistry, a term that has been recycled in recent decades with a completely new meaning. The connection was that the microscopy laboratory was also the place for performing chemical tests. Some contemporary dermatology textbooks referred to technicians in all these fields simply as laboratory men.The end of the 19th century saw lines drawn between microscopy as practiced by pathologists on the one hand and microbiologists on the other. It was Luís del Río y Lara, mentioned earlier, who brought about this change.In dermatology, however, the so-called laboratory men would continue to have broad areas of practice, working as pathologists, microbiologists, and mycologists while at the same time performing serology and other analyses.Microscopy, Microphotography, and Pathologists as DraftsmenThe distinction between microscopy and microphotography (also termed photomicrography) should be underlined. The earliest photoengravings of histologic sections date from the beginning of the 20th century, and the first volume of the journal Actas Dermo-Sifiliográficas (1909–1910) contained several such illustrations.<a class="elsevierStyleCrossRef" href="#bib0075">15</a> Microphotography was a bit of a luxury, however, and early researchers did not routinely use it. This meant that they were obliged to develop competence as draftsmen so they could accurately represent what they saw through their microscopes. Contreras Rubio<a class="elsevierStyleCrossRef" href="#bib0005">1</a> noted that drawing remained a valued skill until very recently. The cultivation of this avocation—or even essential craft—meant that certain classical European dermatopathologists, such as Felix Pinkus and Achille Civatte, became fine draftsmen who left us curious caricatures and miniature works of art in their laboratory notebooks and manuscripts.The Olavides: Father and SonJosé Eugénio de Olavide became familiar with the microscope while still a student. As early as 1857, when he summarized the case of a patient from whom his supervisor, Manuel Soler, had removed a tumor, Olavide remarked that “the microscope revealed only globules of fat.” Mentioned earlier was Olavide and Federico Rubio Galí’s analysis of the air in the wards under Dr Martín de Pedro to see if it contained potentially pathogenic microorganisms; that collaboration led to the 1872 paper by Rubio Galí.<a class="elsevierStyleCrossRef" href="#bib0060">12</a>On his own, Olavide studied fungi under the microscope, sometimes commenting on observations made at magnifications of ×350 and ×700. However, when it was necessary to prepare tissue and other pathologic samples for viewing through a microscope, Olavide relied on Rubio Galí’s expertise.Olavide made it clear in his publications that he was convinced that scabies, filariasis, and tinea were caused by living microorganisms. He was also the most prominent promotor of the concept of plant and animal parasitism in Spanish dermatology at the time. One of his most celebrated experiments—carried out with Rubio Galí and Benito Hernando Espinosa—was successfully transmitting tinea from a patient to 3 animals: a mouse, a cat, and a dog. The mouse died, but the dog escaped, running through the precincts of the hospital—according to Olavide's own account—until a member of the hospital's staff, fearing he would himself be infected, turned the animal loose on the street, where it soon vanished. Olavide's talk on his induction into the Royal National Academy of Medicine and several articles in a Spanish journal covering ophthalmology, venereology, dermatology, and urologic diseases (Revista Especial de Oftalmología, Sifiliografía, Dermatología y Afecciones Urinarias) also dealt with plant and animal parasitism. He argued that contagion was a fundamental concept of pathogenesis, naming 3 types: parasitic, miasmatic, and virulent. Scabies and mycoses provided paradigms for the first type, miasmatic contagion, as Olavide understood it, was related to fermentation. Finally, in virulent contagion, the infectious agent would be a fluid (of a purulent or serous type).José Olavide Malo, José Eugenio's son, was also a dermatologist and a founding member of the Spanish Society of Dermatology. Mentioned earlier was Olavide Malo's doctoral thesis on Trichophyton schoenleinii—classified as Achorion schoenleini at that time.Antonio Mendoza and the Laboratory at Hospital de San Juan de Dios in MadridJosé Eugenio de Olavide was the driving force behind the establishment of the laboratory at Hospital de San Juan de Dios in its first location (Plaza de Antón Martín) and he served as its head, but the true star of this enterprise was one Antonio Mendoza (<a class="elsevierStyleCrossRef" href="#fig0055">Fig. 11</a>), who must not be confused with Antonio Mendoza y Rueda, professor of surgery at the University of Barcelona. Mendoza the microscopist (1848–1917) worked more in microbiology than dermatopathology, and he was particularly expert in methods for isolating and observing germs. It was Olavide who brought Mendoza from Cadiz to Hospital de San Juan de Dios, advancing much of the cost of Mendoza's work out of his own pocket, though he was later reimbursed through funds from the provincial government of Madrid (the Diputación) through the accounts of the public charity hospital (Beneficencia Provincial).<elsevierMultimedia ident="fig0055"></elsevierMultimedia>Mendoza was a highly skilled microbiologist and laboratory technician. Some of his contemporaries—notably Rafael Ariza—considered him to have a weakness for comfort and luxury, but this tendency is easy to understand if we remember the underlying vulnerability of his economic situation. What is certain is that Mendoza's expertise yielded remarkable results in the laboratory. Some decades later, Sainz de Aja<a class="elsevierStyleCrossRef" href="#bib0080">16</a> recounted an anecdote, possibly with slight exaggeration: “When Dr Mendoza presided over the laboratory at Hospital de San Juan de Dios, encouraged by Dr Olavide's progressive, research-oriented spirit, he made this suggestion: ‘What do you think if we put up a sign saying, “Discoveries made in this laboratory this week”?”’This hospital laboratory had cutting edge equipment. In 1880, Maestre de San Juan even mentioned in passing that they had a device for projecting images of what they saw under the microscope<a class="elsevierStyleCrossRef" href="#bib0015">3</a>:“For classroom instruction in our department we use various types of microscopes to examine fresh specimens, and we also have other fixed specimens in collections as well as atlases and line drawings for coloring. In some sessions we use Dr Le Bon's device to project lantern slides. For 8 years we have been using this projector, which is similar to Molteni's, and have added a microscope to the apparatus in a way that magnifies the slides without the preparations becoming damaged by heat. The pharmacy department (Professor Lleget) has the latest model, and there is another one at the histochemistry laboratory at Hospital de San Juan de Dios here in Madrid.”Olavide also noted that Mendoza was commissioned by the authorities to travel abroad in 1884 to study Vibrio cholerae shortly after it was identified by Robert Koch.<a class="elsevierStyleCrossRef" href="#bib0065">13</a>In 1899 a central health facility—the National Institute of Serotherapy, Vaccination and Bacteriology, also commonly referred to as the Alfonso XIII Institute—was founded by merging the National Institute of Hygiene and Bacteriology with the Central Institute of Vaccination. Antonio Mendoza was probably associated with this new facility from the beginning or joined the staff soon after it was founded. At the health spa in Cabreiroá, in the province of Ourense, the visitor can find a plaque giving details of a chemical analysis of the waters, dated 1905. The plaque shows a report of an analysis signed that year by Mendoza in his capacity as member of the staff of the national institute in Madrid (<a class="elsevierStyleCrossRef" href="#fig0060">Fig. 12</a>).<elsevierMultimedia ident="fig0060"></elsevierMultimedia>Finally, the historical sources that Báguena Cervellera<a class="elsevierStyleCrossRef" href="#bib0025">5</a> analyzed in her doctoral thesis revealed that Mendoza was named as second coauthor on 6 publications, more often than any other microbiologist except Jaime Ferrán.Claudio Sala PonsSala Pons merits a place of honor in the history of Spanish dermatopathology, even if only for his collaboration with Juan de Azúa in describing pseudoepithelioma (vegetative pyoderma). I have been unable to learn a great deal about his personal history, but he was a follower of Ramón y Cajal, working with him between 1892 and 1894. In the master's recollections<a class="elsevierStyleCrossRef" href="#bib0085">17</a> there is mention of their collaboration, which led to several titles by Sala Pons on the spinal cord of amphibians (Barcelona, 1890), the cerebral cortex of birds (1893), and his thesis on the glial cells of vertebrates. Ramón y Cajal reported that Sala Pons presented the thesis to a tribunal in Barcelona in 1894.On October 8, 1911, the newspaper ABC announced a course taught by Sala Pons at the faculty of medicine in Madrid. The initial lecture dealt with physical, chemical and microscopy techniques with clinical applications, including the reagents and equipment required by a laboratory.<a class="elsevierStyleCrossRef" href="#bib0090">18</a> The announcement can now be found on the Internet. The monograph on pseudoepithelioma coauthored with Juan de Azúa lists Sala Pons's affiliations as the faculties of pharmacy and sciences; specifically he was full professor in charge of microbiology at the faculty of pharmacy. Another historical document that can be accessed on the Internet mentions Sala Pons's application for the directorship of the Museum of Natural Sciences in Barcelona in February 1921; his candidacy was rejected on the grounds that he was more than 45 years old.<a class="elsevierStyleCrossRef" href="#bib0095">19</a>Sala Pons and de Azúa worked closely together; de Azúa described a complicated case of cutaneous necrobiosis in a young male patient at an international medical conference in Madrid in 1903, using slides prepared by Sala Pons. The case report was published the following year with 2 excellent illustrations of the capillaries of the skin, a venule, and an arteriole. For the same conference, these men prepared another presentation on the possible microbial origin of eczema. However, it is their joint work on pseudoepitheliomas (<a class="elsevierStyleCrossRef" href="#fig0065">Fig. 13</a>) that perhaps marks their main contribution to the literature, as it is one of the first—if not the very first—descriptions of cutaneous pseudomalignancy.<elsevierMultimedia ident="fig0065"></elsevierMultimedia>Juan Sanchez Puente: Pharmacists as MicrobiologistsSanchez Puente, an enigmatic microbiologist working in the early years of the 20th century, became known for a monograph on the human leprosy bacillus published in 1915.<a class="elsevierStyleCrossRef" href="#bib0100">20</a> At that time he was identified as having a PhD in pharmacy and had a post as pharmacist in the public charity hospital (Beneficencia Provincial) of Madrid. He presented himself at the beginning of the book, however, as chief of the laboratory at Hospital de San Juan de Dios in the same city and called himself a follower of Dr Castro, in whose microbiology laboratory he began his study of the leprosy bacillus.Although we have little information about Sánchez Puente, he merits mention in the present article because he belonged to that outstanding group of pharmacists and biologists who have been working in our hospital laboratories for a century now, even though we are often not aware that they initial trained in those disciplines.Pathology and Microbiology in Catalan Dermatology: Juan Giné Partagás and José Viñeta BellaserraJuan Giné Partagás (<a class="elsevierStyleCrossRef" href="#fig0070">Fig. 14</a>) was a prominent figure at the start of Spanish dermatology, working in the field nearly as early as Olavide. His main accomplishment in microscopy was the translation of a 19th-century classic, Rudolf Virchow's great work on cellular pathology. Marco Cuéllar<a class="elsevierStyleCrossRef" href="#bib0015">3</a> notes that Dr Bartolomeu Robert also worked on the translation, which was published by the journal El Pabellón Médico. In 1871 Giné Partagás was named professor of surgery at the faculty of medicine in Barcelona, succeeding Mendoza y Rueda. In the first stage of his professorship his interest centered on normal histology. His work in clinical histology came later. Marco Cuéllar<a class="elsevierStyleCrossRef" href="#bib0015">3</a> notes that his interest in psychiatry toward the end of his life distanced Giné Partagás from histology. Several beautiful engravings illustrating the histologic structure of the skin appeared at the end of his book on surgical dermatology, which also contained clinical photographs.<elsevierMultimedia ident="fig0070"></elsevierMultimedia>Another Barcelona dermatologist, José Viñeta Bellaserra, was born in Havana in 1857. While an undergraduate, he studied with Giné Partagás but later worked with Olavide on his doctorate, which could only be earned in Madrid at that time. Viñeta Bellaserra continued his studies in Paris (Hôpital Saint-Louis), returning to Barcelona to work in 1882. That same year he published a study on cutaneous diphtheria in the journal Revista de Ciencias Médicas, and in 1883 there was a paper describing 5 cases of cutaneous lymphadenia, or Alibert mycosis fungoides. Each was accompanied by histopathologic analysis. Four of the cases were from the time he spent in Paris and the fifth was from Barcelona.Santiago Ramón y Cajal and Pío del Río HortegaIt would be unpardonable to leave the father of Spanish histology out of this account, although it is true that Ramón y Cajal's own contributions to the study of the skin, or dermatopathology, were scarce or nil. He focused his attention so specifically on nerve tissues—about which he made so many magnificent discoveries—that he practically ignored pathology and histology unrelated to the nervous system. However, some of his followers eventually contributed significantly to Spanish dermatopathology, so we can say that he did influence this discipline, but more through his creation of a school of practice than through direct contributions. His new staining methods were influential to a certain degree and were applied to skin samples.Another figure to mention is Pío del Río Hortega. Interestingly, he appeared in 1909 as a founding member of the Spanish Society of Dermatology (later, the Academy). His residence was in Valladolid, and he was therefore listed as a supernumerary member (corresponsal nacional), a category reserved for those who did not live in the capital. Although there are no signs that del Río Hortega participated actively in the sessions of the Society or that he ever published in Actas Dermo-Sifiliográficas, his name appeared regularly on the annual roster of members.Dermatologists, Pathologists, and Microbiologists of the 1920s and 1930sThe 1920s and 1930s were a period of excellence in the history of Spanish science. Authors like Nicolás Calvín, Guillermo de la Rosa King, and Lorenzo Ruiz de Arcaute kept alive the ties between dermatology and the laboratory bench during this time. That all three were serologists reflected the significance of these techniques in venereology after Wasserman's discoveries at the turn of the century. Both de la Rosa and Ruiz de Arcaute were followers of Jorge Francisco Tello,<a class="elsevierStyleCrossRef" href="#bib0105">21</a> although Fernández de la Portilla<a class="elsevierStyleCrossRef" href="#bib0110">22</a> described Ruíz de Arcaute as a direct disciple of Ramón y Cajal.De la Rosa was chief of the pathology department at Hospital de la Princesa de Madrid, and he also worked at the venereal disease clinic of Juan de Azúa (Dispensario Azúa). An obituary in Actas Dermo-Sifiliográficas in 1944 noted that he had studied in New York, thus forging a trail that would be taken by the long and brilliant list of professionals who have since traveled across the Atlantic to complete their training. When Tello won the post of professor of histology and pathology at the faculty of medicine in 1926, Ruiz de Arcaute took up Tello's post at the Hospital Clínico de San Carlos.<a class="elsevierStyleCrossRef" href="#bib0105">21</a> He was also connected with the Alfonso XIII Institute, according to de la Portilla.<a class="elsevierStyleCrossRef" href="#bib0110">22</a> Both men worked with Martínez Anido in a clinic to treat venereal diseases in Madrid, on Calle de Sandoval, where the offices of the Spanish Academy of Dermatology and Venereology (AEDV) were located for several decades. De la Portilla was director of the clinic and Ruiz de Arcaute headed the laboratory. They taught several courses on syphilis, as we know from announcements in dermatology journals of the 1930s. In a 1919 issue of Actas Dermo-Sifiliográficas, Ruíz de Arcaute<a class="elsevierStyleCrossRef" href="#bib0115">23</a> published an article with interesting additional information on pathologic aspects of a case report by Enrique Álvarez Sainz de Aja that had appeared earlier in the same journal. The subject was sclerosis and erythema affecting the fingers. Ruíz de Arcaute died in 1936 when Franco's forces bombed Madrid during the Spanish Civil War.<a class="elsevierStyleCrossRef" href="#bib0105">21</a> These 3 men, who died too soon, were part of a promising generation of laboratory dermatologists.Another small group of dermatologists carried out their own microbiologic and pathology studies. One was Vicente Gimeno, assistant professor of dermatology with Juan de Azúa at the medical faculty in Madrid. In the early years of Actas Dermo-Sifiliográficas, he authored several papers describing his own dermatopathologic research. In a paper on multiple subcutaneous hydatid cysts,<a class="elsevierStyleCrossRef" href="#bib0120">24</a> he set out to discuss “the first slides we prepared for observation under the microscope as well as the photographs taken in the general pathology laboratory of the faculty of medicine.” And in fact the text is accompanied by more than 36 interesting photoengravings. Contemporary accounts also describe José Gay Prieto preparing his own material for histology at least occasionally.<a class="elsevierStyleCrossRef" href="#bib0125">25</a>José Sánchez-Covisa and Julio Bejarano, who carried on the work of Juan de Azúa, presented an interesting study (in French) in Strasbourg in 1923. They had stained skin samples with the same silver nitrate method Ramón y Cajal and his followers had put to such good use for the study of nerves. They did not clarify whether they or their associates at Hospital de San Juan de Dios prepared the samples. Sánchez-Covisa had not yet acceded to the professorship in dermatology he would earn later and through which he would create a strong laboratory under the direction of Manuel Hombría Íñiguez. Stains based on a variety of silver salts must still have seemed novel and attractive in the 1940s. In 1943 Xavier Vilanova and Juan Rubió Roig<a class="elsevierStyleCrossRef" href="#bib0130">26</a> published a modification of del Río Hortega's method for staining epithelial fibrils.The Civil War: Purges and the Case of Manuel Hombría ÍñiguezThe case of Hombría Íñiguez offers an example of how lives were tragically disrupted by the Spanish Civil War, as it affected the population in general and dermatology in particular. Hombría Íñiguez's brother Antonio—also a dermatologist—was executed by Franco's troops when they took Cordoba in 1936, and as Hombría Íñiguez himself was not allowed to work in the public health system or in education, the years following the war were difficult. On December 19, 1940, at the College of Physicians in Madrid, he was declared unfit for posts that required public trust and was not rehabilitated politically until February 8, 1946. His death in 1953 was noted in Actas Dermo-Sifiliográficas. A large number of young physicians who trained in Madrid in the 1930s suffered the same fate and professional ostracism as Hombría Íñiguez. Among those most grievously affected were those who had studied under José Sánchez-Covisa—men like Serviliano Pineda, Covisa's assistant for dermatology and photomicroscopy starting in 1932; Luis Vallejo Vallejo, assistant instructor of serology in the dermatology laboratory; and Emilio Enterría Gaínza.Two Women in the History of Laboratory Dermatology in SpainTwo women who worked in dermatology in Madrid and Barcelona in the 1930s were true pioneers in a field that was otherwise the exclusive province of men at the time. One of them, María de Castro Cantalapiedra, was named in one source as assistant professor for practical sessions under Sánchez-Covisa; however, she was later named in purges and no further information about her has come to light.<a class="elsevierStyleCrossRef" href="#bib0135">27</a> The second woman, who worked in Barcelona, was acknowledged as “Dr J. Salvaus,” the supplier of the anatomic and bacteriologic studies for a 1935 report in Actas Dermo-Sifiliográficas on a case of pseudopelade of Brocq, authored by Xavier Vilanova.<a class="elsevierStyleCrossRef" href="#bib0140">28</a>The Last of the Old Generation: Julio Rodríguez Puchol and Juan Rubió RoigThe immediate predecessors of today's dermatopathologists are 2 outstanding figures: Julio Rodríguez Puchol in Madrid and Juan Rubió Roig in Barcelona. Contreras Rubio<a class="elsevierStyleCrossRef" href="#bib0005">1</a> mentioned them with respect. Rodríguez Puchol was a follower of Tello and later worked with Ramón Martínez in the Hospital del Rey.<a class="elsevierStyleCrossRef" href="#bib0105">21</a> Pérez Peña<a class="elsevierStyleCrossRef" href="#bib0145">29</a> mentions Rodríguez Puchol in his book on physicians who were exiled or purged from the medical faculty in Madrid, although evidence to confirm the report is lacking. According to the author, Rodríguez Puchol was born in Valladolid on April 23, 1912, studied medicine in Madrid in the academic years of 1928–1929 and 1934–1935. His grades were good and he was a student intern in pathology. He graduated with a medical degree on March 23, 1942, receiving his diploma in September 1943 and his PhD in 1960, with a thesis on the histopathology of cutaneous angiitis completed under the guidance of Fernando de Castro. He completed specialist training in histopathology and clinical biochemistry in Valladolid in 1965. As early as 1945, Rodríguez Puchol<a class="elsevierStyleCrossRef" href="#bib0150">30</a> authored a publication in which his affiliation was given as head of the laboratory in the service of Enrique Álvarez Sainz de Aja at Hospital de San Juan de Dios. In that same academic year of 1945–1946, he was listed as a member of the AEDV residing in Madrid (number 7, Calle de Campomanes). Gay Prieto designated him professor of histopathology in the professional studies program in dermatology under his direction at the university. With Contreras Dueñas he served as assistant secretary of the Sixth International Conference of Leprology held in Madrid in 1953 and was then named professor of histopathology in courses on leprology given at the sanatorium in Fontilles, Alicante, under the direction of Gay Prieto and Contreras Dueñas.Félix Contreras Rubio described Rodríguez Puchol as follows in the inaugural talk at the Twenty-Second Conference of the Spanish Society of Pathology (SEAP) in Palma de Mallorca in 2005: “He was a very well rounded general pathologist with specialist knowledge of infectious diseases, endocrinology, cardiology, cytology, and of course dermatology. He served on the Board of Directors of the Spanish Academy of Dermatology for years and was founder and first treasurer of our own Spanish Society of Pathology. Intelligent, hard-working, cultivated, pleasant, and an excellent teacher, he mentored many aspiring pathologists in those early 1960s.” Rodríguez Puchol served as secretary of the AEDV in the 1960s.Juan Rubió Roig was another great name in Spanish dermatopathology in the years after the Civil War. According to Xavier Sierra Valentí, who kindly furnished me with a few personal details, Rubió Roig was born on December 16, 1917, and died on January 21, 2003. His father, Joan Rubió i Bellver, was an important Modernista architect who numbered among the successors of Gaudí. Rubió Roig finished his undergraduate medical studies in 1934. He began working as a teaching assistant at the University of Barcelona in 1965.Contreras Rubio also provided a vivid portrait of Rubió Roig in his opening talk at the SEAP conference: “Joan Rubió was not much interested in presentations, publications, conferences, or public acts of any type. He nonetheless played a significant role in the international courses on surgical pathology organized by Lorenzo Galindo in Barcelona. He was a wonderful person, considerate and accomplished. His many talents included an artistic inclination that he expressed through painting. He was also inventive and designed his own tools, such as a small microtome for surgical biopsies. Another device was an apparatus that automatically embedded samples in paraffin. Manufactured by Myr, S. L. in Barcelona, this processor was the first to be used in Spain when paraffin embedding was just becoming a part of laboratories’ routine practice. In that unforgettable laboratory at the department of dermatology in Barcelona, many generations of dermatologists were taught as well as all the pathologists who chose to spend shorter or longer periods learning from him.”Rubió Roig's career was spent largely in the shadow of Xavier Vilanova, whom he accompanied to posts at the faculties in Valladolid, Valencia, and Barcelona. In some of the papers he authored alone, his credentials were given as departmental histopathologist. In Valencia, he worked alongside José Esteller for a time. Rubió Roig's name first appeared on the roster of the AEDV in Volume 36 (1944–1945), when he was said to reside at number 190 on Via Layetana in Barcelona.From the Age of Pioneers to the PresentAt the beginning of the 20th century, clinical biochemistry—especially serology—and microbiology began to take paths that led them to diverge somewhat from dermatology. The ties between mycology and dermatology, on the other hand, remained close. Dermatologists played notable roles in this field in the 1930s and 1940s. Important were Eduardo de Gregorio in Saragossa in the 1930s and 1940s, and Manuel Pereiro Cuesta and Manuel Pereiro Miguéns (father and son) in Santiago de Compostela.Names in the recent history of dermatopathology are much more familiar to us than those mentioned earlier in this article. Some, like Abelardo Moreno, an outstanding pathologist, and Adolfo Aliaga, a superb dermatologist, are no longer with us. They, along with their generation of mentors in America and other European countries, are the ones who trained the young, rising group of dermatopathologists who work beside us in our laboratories today.Ethical DisclosuresProtection of human and animal subjectsThe author states that no experiments were performed on humans or animals for this investigation.Confidentiality of dataThe author declares that he followed the protocols of his hospital concerning the publication of patient data, and that all the patients mentioned were appropriately informed and gave their written informed consent.Right to privacy and informed consentThe author declares that he obtained informed consent from the patients and/or subjects referred to in this article.Conflicts of InterestThe author declares that he has no conflicts of interest." "textoCompletoSecciones" => array:1 [ "secciones" => array:32 [ 0 => array:2 [ "identificador" => "xres318979" "titulo" => "Abstract" ] 1 => array:2 [ "identificador" => "xpalclavsec301767" "titulo" => "Keywords" ] 2 => array:2 [ "identificador" => "xres318980" "titulo" => "Resumen" ] 3 => array:2 [ "identificador" => "xpalclavsec301768" "titulo" => "Palabras clave" ] 4 => array:2 [ "identificador" => "sec0005" "titulo" => "Introduction" ] 5 => array:2 [ "identificador" => "sec0010" "titulo" => "Sources of Information on Dermatology and the Specialty's Relation to Microbiology and Pathology" ] 6 => array:2 [ "identificador" => "sec0015" "titulo" => "The Early Years of Microscopy: Crisóstomo Martínez, the First of the Spanish Microscopists" ] 7 => array:2 [ "identificador" => "sec0020" "titulo" => "Earliest Microscopic Images of the Skin in the Spanish Medical Literature" ] 8 => array:2 [ "identificador" => "sec0025" "titulo" => "Improvements in Microscopes and Staining Methods in the 19th Century" ] 9 => array:2 [ "identificador" => "sec0030" "titulo" => "Microscopy Arrives in 19th-Century Spain by Way of Paris" ] 10 => array:2 [ "identificador" => "sec0035" "titulo" => "Aureliano Maestre de San Juan" ] 11 => array:2 [ "identificador" => "sec0040" "titulo" => "Federico Rubio Galí" ] 12 => array:2 [ "identificador" => "sec0045" "titulo" => "Salvador Cardenal" ] 13 => array:2 [ "identificador" => "sec0050" "titulo" => "Some Contributions of Spanish Microbiology to Epidemiology" ] 14 => array:2 [ "identificador" => "sec0055" "titulo" => "Plant and Animal Parasitism" ] 15 => array:2 [ "identificador" => "sec0060" "titulo" => "Historically Important Research Facilities" ] 16 => array:2 [ "identificador" => "sec0065" "titulo" => "Microscopy and Histochemistry: Microbiology, Pathology, and Clinical Biochemistry" ] 17 => array:2 [ "identificador" => "sec0070" "titulo" => "Microscopy, Microphotography, and Pathologists as Draftsmen" ] 18 => array:2 [ "identificador" => "sec0075" "titulo" => "The Olavides: Father and Son" ] 19 => array:2 [ "identificador" => "sec0145" "titulo" => "Antonio Mendoza and the Laboratory at Hospital de San Juan de Dios in Madrid" ] 20 => array:2 [ "identificador" => "sec0080" "titulo" => "Claudio Sala Pons" ] 21 => array:2 [ "identificador" => "sec0150" "titulo" => "Juan Sanchez Puente: Pharmacists as Microbiologists" ] 22 => array:2 [ "identificador" => "sec0085" "titulo" => "Pathology and Microbiology in Catalan Dermatology: Juan Giné Partagás and José Viñeta Bellaserra" ] 23 => array:2 [ "identificador" => "sec0090" "titulo" => "Santiago Ramón y Cajal and Pío del Río Hortega" ] 24 => array:2 [ "identificador" => "sec0095" "titulo" => "Dermatologists, Pathologists, and Microbiologists of the 1920s and 1930s" ] 25 => array:2 [ "identificador" => "sec0100" "titulo" => "The Civil War: Purges and the Case of Manuel Hombría Íñiguez" ] 26 => array:2 [ "identificador" => "sec0105" "titulo" => "Two Women in the History of Laboratory Dermatology in Spain" ] 27 => array:2 [ "identificador" => "sec0110" "titulo" => "The Last of the Old Generation: Julio Rodríguez Puchol and Juan Rubió Roig" ] 28 => array:2 [ "identificador" => "sec0115" "titulo" => "From the Age of Pioneers to the Present" ] 29 => array:3 [ "identificador" => "sec0120" "titulo" => "Ethical Disclosures" "secciones" => array:3 [ 0 => array:2 [ "identificador" => "sec0125" "titulo" => "Protection of human and animal subjects" ] 1 => array:2 [ "identificador" => "sec0130" "titulo" => "Confidentiality of data" ] 2 => array:2 [ "identificador" => "sec0135" "titulo" => "Right to privacy and informed consent" ] ] ] 30 => array:2 [ "identificador" => "sec0140" "titulo" => "Conflicts of Interest" ] 31 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2012-07-30" "fechaAceptado" => "2013-01-01" "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec301767" "palabras" => array:4 [ 0 => "History of Spanish dermatology" 1 => "History of Spanish dermatologic pathology" 2 => "History of Spanish microbiology" 3 => "History of Spanish mycology" ] ] ] "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec301768" "palabras" => array:4 [ 0 => "Historia de la dermatología española" 1 => "Historia de la dermatopatología española" 2 => "Historia de la microbiología española" 3 => "Historia de la micología española" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "en" => array:2 [ "titulo" => "Abstract" "resumen" => "Crisóstomo Martínez from Valencia was a pioneering microscopist in 17th-century Europe. The first microscopic representations of skin in Spain appeared in an 18th-century work by Martín Martínez. Microbiology and histopathology progressed considerably in the late 19th century thanks to anatomists like Maestre de San Juan and surgeons like Federico Rubio Galí. The first Spanish pathologist to specialize in dermatology was Antonio Mendoza, a colleague of José Eugenio de Olavide at the Hospital San Juan de Dios in Madrid. Claudio Sala and Juan de Azúa also made significant contributions, including the description of pseudoepithelioma.Several disciples of Santiago Ramón y Cajal and Jorge FranciscoTello, such as Lorenzo Ruiz de Arcaute and Guillermo de la Rosa King, consolidated the dermatology laboratory, but the Civil War sent many into exile or deprived them of their professional status.Juan Rubió in Barcelona and Julio Rodríguez Puchol in Madrid were the immediate predecessors of today's dermatopathologists." ] "es" => array:2 [ "titulo" => "Resumen" "resumen" => "El valenciano Crisóstomo Martínez fue un microscopista pionero en Europa en el siglo xvii. Las primeras representaciones microscópicas cutáneas en España aparecen en una obra del siglo xviii de Martín Martínez.La microbiología y la histopatología progresaron mucho a finales del siglo xix gracias a anatomistas, como Maestre de San Juan, y cirujanos, como Federico Rubio Galí.El primer dermatopatólogo español fue Antonio Mendoza, colaborador de José Eugenio de Olavide en el Hospital de San Juan de Dios de Madrid. Claudio Sala hizo importantes aportaciones con Azúa, como la descripción de los pseudoepiteliomas.Algunos discípulos de Santiago Ramón y Cajal y Jorge Francisco Tello, como Lorenzo Ruiz de Arcaute y Guillermo de la Rosa King, consolidaron el laboratorio dermatológico. La Guerra Civil española llevó a muchos al exilio o a la depuración.Juan Rubió en Barcelona y Julio Rodríguez Puchol en Madrid son los antecesores inmediatos de nuestros actuales dermatopatólogos." ] ] "NotaPie" => array:1 [ 0 => array:2 [ "etiqueta" => "☆" "nota" => "Please cite this article as: del Río E. Los comienzos de la Dermatopatología y de la Microbiología dermatológica en España. Actas Dermosifiliogr. 2014;105:135–149." ] ] "multimedia" => array:14 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1667 "Ancho" => 868 "Tamanyo" => 464627 ] ] "descripcion" => array:1 [ "en" => "Beautiful rendering of a primitive single-lens microscope. This detail, from an engraving by the Valencian microscopist Crisóstomo Martínez, dates from the second half of the 17th century." ] ] 1 => array:7 [ "identificador" => "fig0010" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 997 "Ancho" => 1500 "Tamanyo" => 341787 ] ] "descripcion" => array:1 [ "en" => "Detail from a plate illustrating Martín Martínez's human anatomy text (Anatomía completa del hombre). This small figure is probably the first Spanish depiction of skin as seen under a microscope." ] ] 2 => array:7 [ "identificador" => "fig0015" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 1013 "Ancho" => 1500 "Tamanyo" => 372454 ] ] "descripcion" => array:1 [ "en" => "Three-dimensional rendering of skin (another detail from the same plate <a class="elsevierStyleCrossRef" href="#fig0010">Figure 2</a> was taken from). Some features, such as the branches on hairs, are probably errors or the result of artistic license." ] ] 3 => array:7 [ "identificador" => "fig0020" "etiqueta" => "Figure 4" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr4.jpeg" "Alto" => 1767 "Ancho" => 1200 "Tamanyo" => 610433 ] ] "descripcion" => array:1 [ "en" => "Histologic illustration of skin adnexa from the 1886 dermatology manual (Álbum Clínico de Dermatología) by Jerónimo Pérez Ortiz, published by the journal Revista de Medicina y Cirugía Prácticas. The illustrations were probably versions of those that appeared in other European anatomy textbooks of the period." ] ] 4 => array:7 [ "identificador" => "fig0025" "etiqueta" => "Figure 5" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr5.jpeg" "Alto" => 2227 "Ancho" => 1583 "Tamanyo" => 397257 ] ] "descripcion" => array:1 [ "en" => "Illustration of a Raspail-type microscope and other equipment used by José María Gómez Alamá in practical classes. This plate appeared in an interesting book by this author on dissection (Arte de disecar); this plate is from the second edition of 1872." ] ] 5 => array:7 [ "identificador" => "fig0030" "etiqueta" => "Figure 6" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr6.jpeg" "Alto" => 2206 "Ancho" => 1583 "Tamanyo" => 647603 ] ] "descripcion" => array:1 [ "en" => "Portrait of Aureliano Maestre de San Juan, the first and best example of the Spanish anatomists who pioneered the study of histology in the second half of the 19th century. Maestre de San Juan was the most important histologist working in Spain before Ramón y Cajal." ] ] 6 => array:7 [ "identificador" => "fig0035" "etiqueta" => "Figure 7" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr7.jpeg" "Alto" => 2427 "Ancho" => 1583 "Tamanyo" => 485669 ] ] "descripcion" => array:1 [ "en" => "Illustration of a Nachet-type microscope, taken from Maestre de San Juan's 1879 treatise on general anatomy (Tratado de anatomía general). This microscope was among those favored by Spanish authors of the 1870s and 1880s." ] ] 7 => array:7 [ "identificador" => "fig0040" "etiqueta" => "Figure 8" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr8.jpeg" "Alto" => 1284 "Ancho" => 1000 "Tamanyo" => 236451 ] ] "descripcion" => array:1 [ "en" => "Federico Rubio Galí, an eminent surgeon and good friend of José Eugenio de Olavide. The 2 men collaborated at various points in their careers. This portrait was published in the Spanish anatomy journal edited by Pedro González de Velasco: Anfiteatro Anatómico Español." ] ] 8 => array:7 [ "identificador" => "fig0045" "etiqueta" => "Figure 9" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr9.jpeg" "Alto" => 1903 "Ancho" => 1667 "Tamanyo" => 538408 ] ] "descripcion" => array:1 [ "en" => "Detail of an illustration in José Eugenio de Olavide's atlas of anatomy, showing a liver with syphilitic gummas found in an autopsy performed by Federico Rubio Galí. The histologic section of the lesion is shown above the liver." ] ] 9 => array:7 [ "identificador" => "fig0050" "etiqueta" => "Figure 10" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr10.jpeg" "Alto" => 1836 "Ancho" => 1667 "Tamanyo" => 706226 ] ] "descripcion" => array:1 [ "en" => "Color lithograph from José Eugenio de Olavide's atlas of anatomy, showing an ulcerated, tuberous lesion on the upper eyelid treated by the surgeon Federico Rubio Galí. The fine lines drawn around the eye suggest the flap used to repair the defect. On the right is a rendering of the microscopic view of the histologic specimen prepared by Rubio Galí. It is described as “embryonic tissue: epithelial cells in various stages of formation.”" ] ] 10 => array:7 [ "identificador" => "fig0055" "etiqueta" => "Figure 11" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr11.jpeg" "Alto" => 2436 "Ancho" => 1482 "Tamanyo" => 604638 ] ] "descripcion" => array:1 [ "en" => "Caricature of Antonio Mendoza, microscopist at Hospital de San Juan de Dios in Madrid, published in a magazine of medical satire, El Doctor Sangredo. The egg Mendoza is hatching from is a reference to cytology, and bacteria (cocci, rods, and spirilla) can be seen along the upper edge of the drawing." ] ] 11 => array:7 [ "identificador" => "fig0060" "etiqueta" => "Figure 12" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr12.jpeg" "Alto" => 2500 "Ancho" => 732 "Tamanyo" => 362413 ] ] "descripcion" => array:1 [ "en" => "Plaque commemorating an analysis of the waters at the Cabeiroá spa in Verin, in the province of Ourense, in 1905. Antonio Mendoza signed the analysis in his capacity as a member of the staff of the Instituto Alfonso XIII, directed by Santiago Ramon y Cajal." ] ] 12 => array:7 [ "identificador" => "fig0065" "etiqueta" => "Figure 13" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr13.jpeg" "Alto" => 2586 "Ancho" => 1583 "Tamanyo" => 810048 ] ] "descripcion" => array:1 [ "en" => "Page with figures in the monograph on pseudoepithelioma by Juan de Azúa and Claudio Sala Pons, illustrating this publication's most important contribution: pseudoepitheliomatous hyperplasia." ] ] 13 => array:7 [ "identificador" => "fig0070" "etiqueta" => "Figure 14" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr14.jpeg" "Alto" => 1852 "Ancho" => 1500 "Tamanyo" => 430800 ] ] "descripcion" => array:1 [ "en" => "Portrait of Juan Giné Partagás in the publication Anfiteatro Anatómico Español. A physician with many talents who applied himself to dermatology, surgery, and psychiatry, Giné Partagás published a book on dermatologic surgery that contained beautiful illustrations of the skin." ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:30 [ 0 => array:3 [ "identificador" => "bib0005" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Dermatopatología: ayer, hoy ¿y mañana?" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "F. Contreras Rubio" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Piel" "fecha" => "2003" "volumen" => "18" "paginaInicial" => "463" "paginaFinal" => "467" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0010" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "¿Quién ganó el debate? Reflexiones sobre el futuro de la Dermatopatología" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "J. Basas-Vila" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Actas Dermosifiliogr" "fecha" => "2008" "volumen" => "99" "paginaInicial" => "749" "paginaFinal" => "752" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19091212" "web" => "Medline" ] ] ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib0015" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "La morfología microscópica normal y patológica en la medicina del siglo xix anterior a Cajal. Tesis de doctorado" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "R. Marco Cuéllar" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "1966" "editorial" => "Universidad de Valencia" "editorialLocalizacion" => "Valencia" ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib0020" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:1 [ "referenciaCompleta" => "Garesse R, Sillero A. Obituario. Roberto Marco Cuéllar [cited 2012 Feb 5]. Available from: <a id="intr0010" class="elsevierStyleInterRef" href="http://www.sebbm.com/pdf/157/ob157.pdf">http://www.sebbm.com/pdf/157/ob157.pdf</a>" ] ] ] 4 => array:3 [ "identificador" => "bib0025" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "La introducción de la microbiología en la medicina española del siglo xix. Tesis de doctorado" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "M.J. Báguena Cervellera" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "1983" "editorial" => "Universidad de Valencia" "editorialLocalizacion" => "Valencia" ] ] ] ] ] ] 5 => array:3 [ "identificador" => "bib0030" "etiqueta" => "6" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Los orígenes de la escuela madrileña de dermatología. Tesis de doctorado" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "E. Del Río de la Torre" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "1996" "editorial" => "Universidad Complutense" "editorialLocalizacion" => "Madrid" ] ] ] ] ] ] 6 => array:3 [ "identificador" => "bib0035" "etiqueta" => "7" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "La Anatomía microscópica en España (siglos xvii-xviii)" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "M.L. Terrada Ferrandis" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "1969" "editorial" => "Seminario de Historia de la Medicina Española" "editorialLocalizacion" => "Salamanca" ] ] ] ] ] ] 7 => array:3 [ "identificador" => "bib0040" "etiqueta" => "8" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Álbum clínico de Dermatología" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "J. Pérez Ortiz" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "1886" "editorial" => "Imprenta Nicolás Moya" "editorialLocalizacion" => "Madrid" ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bib0045" "etiqueta" => "9" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Estudios clínicos de Cirujía" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "A. Mendoza Rueda" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "1850" "editorial" => "Imprenta de A. Frexas" "editorialLocalizacion" => "Barcelona" ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bib0050" "etiqueta" => "10" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "De la vénéréologie aux maladies sexuellement transmissibles" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "M. Janier" 1 => "V. Saada" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Ann Dermatol Veneorol" "fecha" => "1989" "volumen" => "116" "paginaInicial" => "957" "paginaFinal" => "964" ] ] ] ] ] ] 10 => array:3 [ "identificador" => "bib0055" "etiqueta" => "11" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "El Achorion Schoenleini. Contribución al estudio de la tiña favosa" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "J. Olavide Malo" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "1888" "editorial" => "Celestino Apaolaza" "editorialLocalizacion" => "Madrid" ] ] ] ] ] ] 11 => array:3 [ "identificador" => "bib0060" "etiqueta" => "12" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Sobre el examen microscópico del vapor atmosférico de la enfermería del Dr. Martín de Pedro en el Hospital General" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "F. Rubio" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "El Siglo Médico" "fecha" => "1872" "volumen" => "19" "paginaInicial" => "404" "paginaFinal" => "407" ] ] ] ] ] ] 12 => array:3 [ "identificador" => "bib0065" "etiqueta" => "13" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Tratamiento curativo y preservativo del cólera" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "(JE). Olavide" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "1884" "editorial" => "Imprenta y estereotipia de El Liberal" "editorialLocalizacion" => "Madrid" ] ] ] ] ] ] 13 => array:3 [ "identificador" => "bib0070" "etiqueta" => "14" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Historia de las enfermedades producidas por hongos" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "X. Sierra" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "1997" "editorial" => "MRA" "editorialLocalizacion" => "Barcelona" ] ] ] ] ] ] 14 => array:3 [ "identificador" => "bib0075" "etiqueta" => "15" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Breve historia de Actas Dermo-Sifiliográficas" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "E. Del Río" 1 => "L. Conde-Salazar" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Actas Dermosifiliogr" "fecha" => "2006" "volumen" => "97" "paginaInicial" => "159" "paginaFinal" => "168" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16796962" "web" => "Medline" ] ] ] ] ] ] ] ] 15 => array:3 [ "identificador" => "bib0080" "etiqueta" => "16" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Discurso del presidente de la Academia. Dr. A Sainz de Aja. Ante mi tercera elección para presidente" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "E.A. Sainz de Aja" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Actas Dermosifiliogr" "fecha" => "1955" "volumen" => "47" "paginaInicial" => "136" "paginaFinal" => "142" ] ] ] ] ] ] 16 => array:3 [ "identificador" => "bib0085" "etiqueta" => "17" "referencia" => array:1 [ 0 => array:1 [ "referenciaCompleta" => "Cajal>Recuerdos de mi vida>Sumario>Apéndice 2 [cited 2012 Jan 31]. Available from: <a id="intr0015" class="elsevierStyleInterRef" href="http://cvc.cervantes.es/ciencia/cajal/cajal_recuerdos/recuerdos/apendice_02.htm">http://cvc.cervantes.es/ciencia/cajal/cajal_recuerdos/recuerdos/apendice_02.htm</a>" ] ] ] 17 => array:3 [ "identificador" => "bib0090" "etiqueta" => "18" "referencia" => array:1 [ 0 => array:1 [ "referenciaCompleta" => "Academias y Centros. Facultad de Medicina. ABC, domingo 8 de octubre de 1911. p. 12. [cited 2012 Jan 31]. Available from: <a id="intr0020" class="elsevierStyleInterRef" href="http://hemeroteca.abc.es/nav/Navigate.exe/hemeroteca/madrid/abc/1911/10/08/012.html">http://hemeroteca.abc.es/nav/Navigate.exe/hemeroteca/madrid/abc/1911/10/08/012.html</a>" ] ] ] 18 => array:3 [ "identificador" => "bib0095" "etiqueta" => "19" "referencia" => array:1 [ 0 => array:1 [ "referenciaCompleta" => "Apendix IV. Dictamen de la ponència nomenada per a informar sobre els merits dels concursants a la plaça de Director del Museu [cited 2012 Jan 31]. Available from: <a id="intr0025" class="elsevierStyleInterRef" href="http://bibdigital.rjb.csic.es/Imagenes/P1101_05/P1101_05_034.pdf">http://bibdigital.rjb.csic.es/Imagenes/P1101_05/P1101_05_034.pdf</a>" ] ] ] 19 => array:3 [ "identificador" => "bib0100" "etiqueta" => "20" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Contribución al estudio de la monografía del bacilo de la lepra humana" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "J. Sánchez Puente" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "1915" "editorial" => "Imprenta Ibérica" "editorialLocalizacion" => "Madrid" ] ] ] ] ] ] 20 => array:3 [ "identificador" => "bib0105" "etiqueta" => "21" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "La Escuela de Cajal. La creación del primer Servicio de Anatomía Patológica en España por D. Francisco Tello" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "F.J. Martínez Tello" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Rev Esp Patol" "fecha" => "2002" "volumen" => "35" "paginaInicial" => "475" "paginaFinal" => "480" "itemHostRev" => array:3 [ "pii" => "S0140673613610971" "estado" => "S300" "issn" => "01406736" ] ] ] ] ] ] ] 21 => array:3 [ "identificador" => "bib0110" "etiqueta" => "22" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Nota necrológica. Lorenzo Ruiz de Arcaute" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "J. Fernández de la Portilla" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:4 [ "tituloSerie" => "Actas Dermosifiliogr" "fecha" => "1938" "paginaInicial" => "29549" "paginaFinal" => "29550" ] ] ] ] ] ] 22 => array:3 [ "identificador" => "bib0115" "etiqueta" => "23" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Estudio anatomopatológico del caso de eritematoesclerosis de los dedos publicado por el Dr. Sainz de Aja en el número 2" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "L. Ruiz de Arcaute" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Actas Dermosifiliogr" "fecha" => "1919" "volumen" => "10" "paginaInicial" => "189" "paginaFinal" => "191" "itemHostRev" => array:3 [ "pii" => "S0140673613610831" "estado" => "S300" "issn" => "01406736" ] ] ] ] ] ] ] 23 => array:3 [ "identificador" => "bib0120" "etiqueta" => "24" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Caso notable de hidátides múltiples en el tejido celular subcutáneo" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "V. Gimeno" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Actas Dermosifiliogr" "fecha" => "1915" "volumen" => "7" "paginaInicial" => "29" "paginaFinal" => "68" ] ] ] ] ] ] 24 => array:3 [ "identificador" => "bib0125" "etiqueta" => "25" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Epitelioma por rayos x sobre lupus eritematoso" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "R. Hombría" 1 => "J. Soto" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Actas Dermosifiliogr" "fecha" => "1930" "volumen" => "22" "paginaInicial" => "387" "paginaFinal" => "390" ] ] ] ] ] ] 25 => array:3 [ "identificador" => "bib0130" "etiqueta" => "26" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Modificaciones a la técnica de Río Hortega para la demostración de las epiteliofibrillas" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "X. Vilanova" 1 => "J. Rubió" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Actas Dermosifiliogr" "fecha" => "1943" "volumen" => "34" "paginaInicial" => "258" "paginaFinal" => "262" ] ] ] ] ] ] 26 => array:3 [ "identificador" => "bib0135" "etiqueta" => "27" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "La destrucción de la Ciencia en España. Depuración universitaria en el franquismo" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "L.E. Otero Carvajal" 1 => "M. Núñez Díaz-Balart" 2 => "G. Gómez Bravo" 3 => "J.M. López Sánchez" 4 => "R. Simón Arce" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "2007" "editorial" => "Editorial Complutense" "editorialLocalizacion" => "Madrid" ] ] ] ] ] ] 27 => array:3 [ "identificador" => "bib0140" "etiqueta" => "28" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Estudio clínico, anatómico y bacteriológico de un caso de pseudopelada de Brocq" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "X. Vilanova" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Actas Dermosifiliogr" "fecha" => "1935" "volumen" => "27" "paginaInicial" => "531" "paginaFinal" => "542" ] ] ] ] ] ] 28 => array:3 [ "identificador" => "bib0145" "etiqueta" => "29" "referencia" => array:1 [ 0 => array:1 [ "referenciaCompleta" => "Pérez Peña F. Exilio y depuración política (en la facultad de Medicina de San Carlos). Madrid. Visionet, 2005 [cited 2012 Feb 15]. Available from: <a id="intr0035" class="elsevierStyleInterRef" href="http://www.dermocosmos.com/espanol/libros/Exilio%20y%20depuracion%2015.htm">http://www.dermocosmos.com/espanol/libros/Exilio%20y%20depuracion%2015.htm</a>" ] ] ] 29 => array:3 [ "identificador" => "bib0150" "etiqueta" => "30" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Balanitis sifilítica" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "J.R. Puchol" 1 => "P. Gómez" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Actas Dermosifiliogr" "fecha" => "1945" "volumen" => "36" "paginaInicial" => "364" "paginaFinal" => "370" ] ] ] ] ] ] ] ] ] ] ] "idiomaDefecto" => "en" "url" => "/15782190/0000010500000002/v1_201403090058/S1578219014000092/v1_201403090058/en/main.assets" "Apartado" => array:4 [ "identificador" => "6153" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "History and Humanities in Dermatology" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/15782190/0000010500000002/v1_201403090058/S1578219014000092/v1_201403090058/en/main.pdf?idApp=UINPBA000044&text.app=https://actasdermo.org/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1578219014000092?idApp=UINPBA000044"]

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History and Humanities in Dermatology

The Beginnings of Dermatopathology and Dermatologic Microbiology in Spain

Los comienzos de la Dermatopatología y de la Microbiología dermatológica en España

E. del Río

Clínica Dermalar, Santiago de Compostela, Spain

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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Medicine is multidisciplinary by nature&#44; but the characteristics of each discipline&#39;s store of knowledge and its peculiar requirements mean that some branches have more in common than others&#46; Dermatology is especially dependent on microbiology and above all on pathology&#44; to the extent that we remain unable to conceive of good clinical practice in our specialty without their support&#46; This is the case even as our need for knowledge of genetics and immunology becomes as great or greater&#46; By the second half of the 19th century&#44; both microbiology and pathology had already taken their familiar forms&#46; Their practices developed somewhat later than those of dermatology itself&#44; whose origins can be traced to the beginning of the same century&#46;</p><p id="par0010" class="elsevierStylePara elsevierViewall">Thus&#44; the 3 specialties emerged and developed in Spain almost simultaneously&#46; All were in evidence in the second half of the 19th century and were well established by the first third of the 20th&#46; In fact&#44; dermatology&#8212;through the pioneering laboratory of Hospital de San Juan de Dios in Madrid&#8212;provided essential support for the advancement of histopathology and microbiology&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">This article reviews the fundamental principles&#44; history&#44; and interrelations that brought pathology&#44; microbiology&#44; and dermatology together in Spain&#44; covering the early period of emergence and consolidation in the second half of the 19th century and their coming of age in the first half of the 20th&#46; As the story has been little studied and microbiologists&#44; pathologists&#44; and dermatologists are not fully aware of it&#44; this record represents a modest effort to pay homage to the historical contribution of the laboratory technicians whose work provided the background for clinical practice&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Sources of Information on Dermatology and the Specialty&#39;s Relation to Microbiology and Pathology</span><p id="par0020" class="elsevierStylePara elsevierViewall">Very few studies have explored the relations among the 3 disciplines under consideration here&#46; Ten years ago&#44; F&#233;lix Contreras Rubio<a class="elsevierStyleCrossRef" href="#bib0005"><span class="elsevierStyleSup">1</span></a> published a brief overview of the recent history of dermatopathology in Spain&#44; and Julio Bassas-Vila<a class="elsevierStyleCrossRef" href="#bib0010"><span class="elsevierStyleSup">2</span></a> has also provided general reflections on the subject&#46;</p><p id="par0025" class="elsevierStylePara elsevierViewall">A key secondary source of information about the early period of Spanish pathology is the doctoral thesis of Roberto Marco Cu&#233;llar&#44;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> written under the supervision of Professor Jos&#233; Mar&#237;a L&#243;pez Pi&#241;ero in Valencia and completed in 1966&#46; This excellent thesis was rigorous and almost encyclopedic in its scope&#44; as it delved into the emergence and early development of pathology before Ram&#243;n y Cajal&#46; Surprisingly&#44; it has never been published&#44; although Marco Cu&#233;llar took some material from his thesis for a chapter on histology and cytology in the fifth volume of the monumental history of medicine edited by Pedro La&#237;n Entralgo &#40;<span class="elsevierStyleItalic">Historia Universal de la Medicina</span>&#41;&#46; After receiving degrees in both chemistry and medicine&#44; Marco Cu&#233;llar went on to earn his PhD in medicine&#46; He then began a career in biochemistry and in 1984 became full professor of that subject at the Universidad Aut&#243;noma de Madrid&#46; His career pathway perhaps explains the limited public dissemination of his thesis on the history of medicine&#46; Marco Cu&#233;llar died on June 27&#44; 2008&#46;<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a></p><p id="par0030" class="elsevierStylePara elsevierViewall">The history of microbiology in Spain has been thoroughly studied by Mar&#237;a Jos&#233; B&#225;guena Cervellera&#44;<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> whose thesis on that subject was also supervised by L&#243;pez Pi&#241;ero&#46; This thesis likewise remains unpublished&#44; even though it is rigorous and the amount of information it holds makes it worthy of wider circulation and publication&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">Finally&#44; the present author&#39;s doctoral thesis on the history of dermatology&#44;<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a> completed in 1996 under the supervision of Professor Antonio Garc&#237;a P&#233;rez&#44; covered the origins of the school of dermatologic theory and practice in Madrid&#44; the first to appear in this field in Spain&#46; This thesis was based on a considerable number of primary sources and original documents&#46;</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">The Early Years of Microscopy&#58; Cris&#243;stomo Mart&#237;nez&#44; the First of the Spanish Microscopists</span><p id="par0040" class="elsevierStylePara elsevierViewall">Early microscopes are said to have been mainly used to entertain rather than to serve medical purposes&#46; Microscopes were available as early as the first decades of the 17th century&#44; but it was the curiosity of a Dutch merchant&#44; Antonie van Leeuwenhoek&#44; that led him to polish lenses and design instruments capable of magnification &#215;240&#44; thus opening up a view of a previously unimagined world and making real progress possible&#46; The first to see ciliates and other protozoa in water&#44; van Leeuwenhoek called them animalcules and gave a full account of his observations in letters to the Royal Society of London&#59; he also saw sperm cells&#44; although he did not form a clear notion of their significance&#44; and he may have seen red blood cells and bacteria&#46; Nearly 2 centuries would have to pass&#44; however&#44; before this knowledge would be applied in medicine&#46;</p><p id="par0045" class="elsevierStylePara elsevierViewall">Robert Hooke&#44; who was nearly a contemporary of van Leeuwenhoek&#44; authored an interesting treatise titled <span class="elsevierStyleItalic">Micrographia</span>&#44; published in 1665&#46; Another noteworthy 17th-century user of microscopes was the Italian Marcelo Malpighi&#46; His name is familiar to us today because of an eponymous term&#58; the Malpighian layer of the skin&#44; referring to the nucleated basal&#44; spinous&#44; and granular strata of the epidermis&#44; excluding the stratum corneum&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">Among the first Spaniards to use the new instrument was Cris&#243;stomo Mart&#237;nez &#40;1638&#8211;1694&#41;&#44; who was a member of the first generation of microscopists that included van Leeuwenhoek and Hooke&#46; Mart&#237;nez left a beautiful collection of engravings showing the structure of bone&#46; In a still life decorating the edge of one of the anatomical illustrations&#44; there is a single-lens microscope &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>&#41;&#46; This detail was used on the front cover of a monograph on the history of microscopic anatomy in 17th- and 18th-century Spain by Mar&#237;a Luz Terrada Ferrandis&#46;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a></p><elsevierMultimedia ident="fig0005"></elsevierMultimedia></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Earliest Microscopic Images of the Skin in the Spanish Medical Literature</span><p id="par0055" class="elsevierStylePara elsevierViewall">Mart&#237;n Mart&#237;nez&#44; a celebrated Spanish physician at the end of the 17th century and beginning of the 18th century&#44; was responsible for what were probably the first illustrations of the skin as seen under a microscope to be published in Spain&#46; The 2 small illustrations appeared in a 1728 human anatomy book&#44; inserted into the upper corners of a plate&#46; The one depicting a reticulated pattern in the upper right corner &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41; was described thus by Mart&#237;nez as showing &#8220;a portion of the skin with its epidermis&#46;&#8221; The other drawing &#40;<a class="elsevierStyleCrossRef" href="#fig0015">Fig&#46; 3</a>&#41;&#44; which was also given the number 3 in the source text&#44; was said to represent &#8220;the manufacture of the skin&#44; as revealed under the microscope&#58; A&#46; Cutaneous glands&#46; B&#46; Nerve papillae&#46; C&#46; Pores&#46; D&#46; Hairs&#46; E&#46; Subcutaneous vessels&#46;&#8221; Exaggerated bumps appear on the surface of the skin and the hairs have very unrealistic branches&#59; 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This was the clinical manual &#40;<span class="elsevierStyleItalic">&#225;lbum</span>&#41; on dermatology by Jer&#243;nimo P&#233;rez Ortiz&#44;<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">8</span></a> published in 1886&#46; Neither author seems to have prepared the histologic sections that illustrate their work&#44; however&#46; Rather&#44; they seem to have reproduced illustrations and borrowed information from books already in print elsewhere in Europe&#46;</p><elsevierMultimedia ident="fig0020"></elsevierMultimedia></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Improvements in Microscopes and Staining Methods in the 19th Century</span><p id="par0065" class="elsevierStylePara elsevierViewall">The methods used in microscopy advanced relatively little in the 18th century&#46; Instruments were simple single-lens devices that could reveal little more than van Leeuwenhoek had managed to see&#46; 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Lesson 19 covered both simple and compound microscopes and their main accessories&#44; such as diaphragms&#44; condensers&#44; the camera obscura and the camera lucida &#40;or camera clara&#41;&#44; prisms&#44; etc&#46; Mendoza y Rueda also discussed how objects are prepared for viewing&#8212;using the methods of Purkinje&#44; Hannover&#44; Jacobson and Raspail&#8212;as well as the double-bladed knife developed by Valentin for cutting thin sections&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">Another key advance was the move away from reflected light toward better systems of specimen illumination&#44; which compound microscopes required&#46; The middle quarters of the 19th century witnessed great improvements in microtomes&#44; illumination&#44; and staining methods&#46;</p><p id="par0080" class="elsevierStylePara elsevierViewall">Specimen dissection was the subject of a book by Jos&#233; Mar&#237;a G&#243;mez Alam&#225; &#40;<span class="elsevierStyleItalic">Arte de disecar</span>&#41;&#46; Its second edition &#40;1872&#41; included important references to different types of single-lens and compound microscopes&#46; Included were solar microscopes and models incorporating early arc lamps&#46; He also discussed the various types of micrometers &#40;some with threaded screws&#44; others with scales etched into glass disks&#41;&#44; and set out guidelines for achieving adequate illumination of objects by reflection or transmission&#46; G&#243;mez Alam&#225; named 2 conditions to meet when preparing tissue samples&#58; the section must be extremely thin and the surfaces must be perfectly flat&#46; To help readers achieve both requirements&#44; he gave advice on managing scalpels&#44; fine saws&#44; and polishing stones for bones or minerals&#46; Marco Cu&#233;llar<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> reports that he mentioned an early version of a microtome by Straus-Durchen &#40;sic&#46;&#41;&#44; a reference to Strauss-D&#252;rckheim&#46; The book contains illustrations of various instruments used at the time &#40;<a class="elsevierStyleCrossRef" href="#fig0025">Fig&#46; 5</a>&#41;&#46;</p><elsevierMultimedia ident="fig0025"></elsevierMultimedia><p id="par0085" class="elsevierStylePara elsevierViewall">This same period saw the introduction of a well-known staining technique for classifying bacteria into 2 broad groups&#46; The system is the same one we use today and was proposed in 1884 by Hans Christian Gram of Denmark&#46;<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a></p><p id="par0090" class="elsevierStylePara elsevierViewall">Early methods for working with microscopes grew out of a great deal of experimentation and required skilled craftsmanship&#46; One of the most interesting accounts of the process of staining samples for microscopy at the end of the 19th century was written in the field of dermatology&#44; specifically the doctoral thesis on <span class="elsevierStyleItalic">Achorion schoenleinii</span> and tinea favosa&#44; by Jos&#233; Olavide Malo&#44;<a class="elsevierStyleCrossRef" href="#bib0055"><span class="elsevierStyleSup">11</span></a> the son of Jos&#233; Eugenio de Olavide Landaz&#225;ba&#46; The thesis&#44; which was published in 1888&#44; gave a detailed description of how fungal samples were prepared&#46; First the material was placed in an antiseptic solution &#40;mercury chloride at a concentration of 1 part per 1000&#41; for 2<span class="elsevierStyleHsp" style=""></span>minutes&#46; It was then inserted into a tube with gelatin&#44; cooled to 40<span class="elsevierStyleHsp" style=""></span>&#176;C and cooled further under running water until the gel had solidified&#44; and finally incubated at a temperature of 27<span class="elsevierStyleHsp" style=""></span>&#176;C to 28<span class="elsevierStyleHsp" style=""></span>&#176;C&#46; Fungal colonies appeared in 6 to 8 days and could then be studied under a microscope&#46; Olavide Malo proposed 5 steps&#44; and they are quoted here for the historical interest of the description&#58;</p><p id="par0095" class="elsevierStylePara elsevierViewall">&#8220;1st&#44; the organic matter is softened in an alkaline solution&#44; preferably one containing potassium&#59; 2nd&#44; oils are removed by treating the fungus with alcohol followed by sulfuric ether or chloroform or any other solvent of oils&#59; 3rd&#44; portions of the vegetable material is stained&#58; one should choose potassium&#44; to give a blue color&#44; or eosin in water&#44; or Bismarck brown&#46;&#46;&#46;&#59; 4th&#44; the vegetable material is then treated with a very strong solution &#40;equal parts&#41; of potassium acetate and distilled water in order to dissolve the excess color taken up by the bulk of the primitive stem so that the tissue is more transparent&#59; and 5th&#44; the sample is finally fixed on a slide&#58; instead of Canada balsam&#44; use a thick gum in solution&#44; adding a few drops of phenic acid to prevent the fungi from escaping into the air&#46;&#8221;</p><p id="par0100" class="elsevierStylePara elsevierViewall">Olavide Malo went on to explain how he undertook mycologic studies by growing the samples to increase the volume and embedding the material in paraffin &#40;as well as in gelatin and in soap&#44; although these media were judged inferior to paraffin&#41; for later slicing with the freezing microtome introduced by Thomas Taylor or the rocking microtome manufactured by the Cambridge Instrument Company&#46; Of the second microtome&#44; the author says&#44; &#8220;It is very appropriate for this type of work&#44; providing an excellent series of slices with paraffin edges that line up squarely as they leave the microtome on a conveyor belt some 50 or 60<span class="elsevierStyleHsp" style=""></span>cm long&#46;&#8221;</p><p id="par0105" class="elsevierStylePara elsevierViewall">A year before Olavide Malo presented his thesis to the tribunal&#44; Julio Magraner Marinas<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> had already written the following summary of a quarter of a century of work in this field&#58;</p><p id="par0110" class="elsevierStylePara elsevierViewall">&#8220;Work done with microscopes over the past 25 years has revealed that animals contain within themselves numerous colonies of living organisms&#59; although many of these organisms are harmless&#44; some are closely joined to the existence and production of various diseases&#46; It was a question of experimenting&#44; and now our experiments have answered in the affirmative&#46;&#8221;</p><p id="par0115" class="elsevierStylePara elsevierViewall">Within a few years&#44; he would be proven right&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Microscopy Arrives in 19th-Century Spain by Way of Paris</span><p id="par0120" class="elsevierStylePara elsevierViewall">Most of our knowledge of histology and pathology dates from the period between 1825 and 1848&#46; The main contributors were German scientists like Henle&#44; Purkinje&#44; M&#252;ller&#44; Schwann&#44; and Schleiden&#46; Soon after this period&#44; the circle that formed around Rudolf Virchow would play a key role&#46; However&#44; modern microscopic methods traveled to 19th-century Spain by way of Paris&#46; Interestingly&#44; a Venezuelan microscopist&#8212;Eloy Carlos Ord&#243;&#241;ez Sordo&#44; whom most sources refer to simply as Ord&#243;&#241;ez&#8212;was closely linked to the development of techniques in Spain&#46; Ord&#243;&#241;ez Sordo was born in 1822 in Gir&#243;n&#44; a village in the province of Pamplona in Nueva Granada&#44; one of the 3 republics of the old state of Gran Colombia&#46; His parents were from Maracaibo&#44; Venezuela&#44; and he studied in Santa Fe de Bogot&#225;&#46; After graduation&#44; he accompanied Archbishop Mosquera on a journey from Colombia to New York&#44; where he stayed for 2 years&#46; On the archbishop&#39;s death&#44; Ord&#243;&#241;ez Sordo settled in Paris and devoted himself to the study of microscopy&#44; probably from 1851 onward&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> He died on May 19&#44; 1868&#44; at the age of 46 years&#46;</p><p id="par0125" class="elsevierStylePara elsevierViewall">In discussing dermatopathology&#44; Marco Cu&#233;llar<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> cites the 1858 French publications of Ord&#243;&#241;ez Sordo&#58; a report describing a pigmented tumor&#8212;probably a melanoma&#8212;in the <span class="elsevierStyleItalic">Bulletin de la Soci&#233;t&#233; d&#8217;Anatomie</span>&#59; a report to the Society of Biologists in France on an indurated chancre on the foreskin which was reviewed in the Spanish bulletins <span class="elsevierStyleItalic">El Siglo M&#233;dico</span> and <span class="elsevierStyleItalic">El Compilador M&#233;dico</span>&#59; and another 1863 report on hair muscles written for the same biology association&#46;</p><p id="par0130" class="elsevierStylePara elsevierViewall">Ordo&#241;ez Sordo proposed a system for classifying tissues into 16 groups&#44; which Aureliano Maestre de San Juan included in his book on general anatomy&#46; Marco Cu&#233;llar<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> lists them as fibrillar &#40;cellular&#44; laminar&#44; or conjunctival&#41;&#44; fibrous &#40;transient&#44; fibro-plastic&#41;&#44; elastic&#44; adipose&#44; cartilaginous&#44; osseous&#44; animal muscular &#40;striated muscle&#41;&#44; organic muscular &#40;smooth muscle&#41;&#44; epithelial&#44; hyaline or anhistic&#44; pigmented &#40;lamina fusca&#44; choroid&#44; skin&#41;&#44; retinal&#44; nervous&#44; enamel &#40;of teeth&#41;&#44; ivory &#40;also of teeth&#41;&#44; and crystalline&#46;</p><p id="par0135" class="elsevierStylePara elsevierViewall">Two men who studied under Ord&#243;&#241;ez Sordo&#8212;Aureliano Maestre de San Juan and Federico Rubio Gal&#237;&#8212;established schools of practice in the last quarter of the 19th century&#46; The first was associated largely with university laboratories and was the result of Maestre de San Juan&#39;s interest in the pure study of morphology and histology&#44; in keeping with his training as an anatomist&#46; The second reflected Rubio Gal&#237;&#8217;s more clinical inclinations&#46; His work involved a less constrained style of extramural education and leaned more clearly in the direction of pathology&#46;</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Aureliano Maestre de San Juan</span><p id="par0140" class="elsevierStylePara elsevierViewall">An eminent anatomist&#44; Maestre de San Juan &#40;<a class="elsevierStyleCrossRef" href="#fig0030">Fig&#46; 6</a>&#41; established a solid foundation for histology in Spain&#44; according to the account of Marco Cu&#233;llar&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> He largely renounced research that could lead to original contributions in order to carry out the more mundane task of building up a store of information&#46; Thus&#44; he saw himself more as a laboratory technician than a scholar&#44; and he shared his knowledge with his contemporaries&#46; Born in Granada on October 17&#44; 1828&#44; he took his degree in the first stage of medical studies &#40;<span class="elsevierStyleItalic">bachillerato</span>&#41; in the same city and then spent 3 years completing his medical education in Madrid&#44; where he graduated on November 8&#44; 1847&#44; at the age of 19 years&#46; He received his doctorate in Madrid in 1851 and returned to Granada in 1856 to work as a clinical professor in the university&#39;s faculty of medicine&#46; In 1860 he competed for a professorship in general anatomy in Granada and occupied that position until he returned to Madrid in 1873 to take up a full professorship in normal and pathologic histology&#46; A laboratory accident caused chemical burns that cost him his sight in 1888&#44; and he died in Alicante on June 1&#44; 1890&#46; Maestre de San Juan lived simply&#44; spending most of his money on books and materials for his work with the microscope&#46;</p><elsevierMultimedia ident="fig0030"></elsevierMultimedia><p id="par0145" class="elsevierStylePara elsevierViewall">One of this anatomist&#39;s most important contributions was to found the Histologic Society on February 22&#44; 1874&#44; in the company of Andr&#233;s del Busto&#44; Miguel Colmeiro&#44; Gabriel de la Puerta&#44; and the Venezuelan Francisco Jos&#233; Delgado Jugo&#44; among others&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> None of these men were engaged with the fields of dermatopathology or skin microbiology&#44; probably because they felt no need to confine themselves to such specific areas at this time&#46; The society they founded was short-lived&#8212;it disappeared after 3 years&#8212;but the section on histology of the Academy of Physicians and Surgeons of Madrid carried the project forward&#46; The formation of the Histologic Society was thus an important step toward laying a solid foundation for microscopic research in Spain&#46;</p><p id="par0150" class="elsevierStylePara elsevierViewall">Marco Cu&#233;llar<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> reports that in 1879 Maestre de San Juan published a treatise on normal and pathologic histology that included a discussion of laboratory technique &#40;<span class="elsevierStyleItalic">Tratado elemental de Histolog&#237;a normal y patol&#243;gica precedido de un resumen de t&#233;cnica de laboratorio</span>&#41;&#46; A second&#44; extensively revised edition published in 1885 dealt with the compound microscope in greater depth and gave a good discussion of the Natchet &#40;<a class="elsevierStyleCrossRef" href="#fig0035">Fig&#46; 7</a>&#41; and Verick microscopes&#44; the camera lucida&#44; micrometers&#44; polarization devices&#44; goniometers&#44; immersion lenses&#44; correction&#44; heated slides and other specimen holders&#44; rotating components&#44; mounts for dissection&#44; prism inverters&#44; the Abbe condenser&#44; and the spectroscope&#46; A section on chemicals&#44; vehicles&#44; and reagents covered harmless vehicles &#40;water&#44; physiologic serum&#44; amniotic fluid&#41;&#44; reagents &#40;alcohol and acetic&#44; chromic&#44; picric and other acids&#41;&#44; preserving agents &#40;glycerin&#44; turpentine&#41;&#44; cements &#40;bitumen of Judea&#44; Zeigler&#39;s bitumen&#41;&#44; stains that impregnate tissues &#40;silver nitrate&#44; gold or palladium chloride&#44; osmic acid&#41;&#44; and stains that act by inhibition &#40;carmine&#44; picrocarminate&#44; Ranvier purpurin&#44; sulfate&#44; and rosaniline acetate&#44; fuchsine or aniline red&#44; quinoline blue&#44; hematoxylin&#44; indigo carmine&#44; eosin&#44; and more&#41;&#46; Also discussed were preserving and clearing agents such as glycerin&#44; turpentine&#44; Canada balsam&#44; clove oil&#44; and dammar resin&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a></p><elsevierMultimedia ident="fig0035"></elsevierMultimedia><p id="par0155" class="elsevierStylePara elsevierViewall">Although Maestre de San Juan initially hesitated to align himself with a particular approach&#44; he finally opted to follow Rudolf Virchow&#39;s German school of histology&#44; while still conscious of the debt he owed his French training&#46; He used&#44; for example&#44; a Ranvier microtome&#44; which was little more than a screw and shaving razor&#46; Methods for fixing samples were being introduced at this time&#46; Initially&#44; gum arabic was used&#46; Freezing was also discussed&#44; although it was felt to be difficult to find equipment that could achieve the desired result&#46;</p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0060">Federico Rubio Gal&#237;</span><p id="par0160" class="elsevierStylePara elsevierViewall">Another key figure&#44; was Rubio Gal&#237; &#40;<a class="elsevierStyleCrossRef" href="#fig0040">Fig&#46; 8</a>&#41;&#44; a histologist and surgeon whose interest in the microscope dated from the time he spent working in Seville&#46; There&#44; he joined a group of surgeons who used histologic techniques themselves or took an interest in them&#46; Others in this circle&#44; Marco Cu&#233;llar<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> reports&#44; were Adolfo Moreno Pozo&#44; Juan Creus Manso&#44; Santiago Gonz&#225;lez Encinas&#44; and Jos&#233; Calvo Mart&#237;n&#46;</p><elsevierMultimedia ident="fig0040"></elsevierMultimedia><p id="par0165" class="elsevierStylePara elsevierViewall">Rubio Gal&#237; was born in Puerto de Santa Mar&#237;a in 1827 and studied medicine in Cadiz&#44; graduating in 1850 with high marks&#46; He won a post at the Hospital Central de Sevilla that same year&#44; but his political liberalism led to serious professional setbacks&#46; At one point he had to give classes in fencing&#8212;a skill he excelled at&#8212;in order to make ends meet&#46; His interest in histology began during a period of exile to France&#44; where he became interested in Ord&#243;&#241;ez Sordo&#44; according to Marco Cu&#233;llar&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> He returned to Seville for a time and later worked in Madrid&#46;</p><p id="par0170" class="elsevierStylePara elsevierViewall">In 1872 he authored a paper on an analysis of air in the hospital wards of Dr Ezequiel Mart&#237;n de Pedro in the Hospital General de Madrid<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">12</span></a>&#59; the analysis was accomplished in collaboration with Jos&#233; Eugenio de Olavide&#46; They found various bacteria and described 3 types&#58; bacterium punctum &#40;which we would refer to as cocci today&#41;&#44; bacterium catenula &#40;chains of 2&#8212;i&#46;e&#46;&#44; diplococci&#8212;or 3 cocci&#41;&#44; and bacterium baculum &#40;bacilli&#41;&#46; The publication even described how these bacteria moved&#46;</p><p id="par0175" class="elsevierStylePara elsevierViewall">Most of the histologic preparations for the autopsies Olavide later described in his anatomic atlas were done by Rubio Gal&#237;&#46; Some of these preparations&#44; even made it possible to supplement the color plates of the atlas with black and white illustrations of clinical lesions &#40;<a class="elsevierStyleCrossRefs" href="#fig0045">Figs&#46; 9 and 10</a>&#41;&#46;</p><elsevierMultimedia ident="fig0045"></elsevierMultimedia><elsevierMultimedia ident="fig0050"></elsevierMultimedia></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Salvador Cardenal</span><p id="par0180" class="elsevierStylePara elsevierViewall">Born in Valencia in 1852&#44; Salvador Cardenal studied to be a surgeon in Barcelona&#44; graduating in 1875&#46; Cardenal&#39;s report on histologic and clinical characteristics of lupus&#44; epithelioma&#44; and ulcerating tumors&#44; which also compared their different treatments&#44; won a competition organized by the Royal Academy of Medicine in Madrid&#46; Marco Cu&#233;llar<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> notes that the report revealed Cardenal&#39;s considerable understanding of histologic techniques and a wide range of stains and fixing agents&#46; Cardenal recommended carminic acid with the addition of picric acid to stain epithelioma samples&#44; for example&#46; He also published a manual on surgical antisepsis&#44; of which several editions were printed&#46;</p><p id="par0185" class="elsevierStylePara elsevierViewall">Cardenal&#39;s work gave evidence of the many advances that led to enormous growth in knowledge in the field of microbiology in the last third of the 19th century&#44; according to B&#225;guena Cervellera&#46;<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> Relevant advances included improvements in the manufacture of microscopes &#40;better objectives and apochromatic light-condensing lenses&#41; and the use of aniline dyes to stain microorganisms&#46;</p></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Some Contributions of Spanish Microbiology to Epidemiology</span><p id="par0190" class="elsevierStylePara elsevierViewall">The study of infectious diseases was an independently progressing field that furthered the development of microscopy&#44; as presented by B&#225;guena Cervellera<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> in her thesis&#46; Pablo Colv&#233;e&#44; for example&#44; demonstrated the means of contagion during an epidemic of trichinosis in the town of Villar del Arzobispo in 1877&#46; Vicente Peset Cervera&#44; who had studied both medicine and chemistry&#44; published on the role of fermentation in physiology and pathology in 1878&#46; His meticulous studies of the fermentation of foods in the digestive tract followed a line similar to Pasteur&#39;s&#46; From this work it was possible to infer the existence of microorganisms that promoted the digestive process&#44; ultimately undermining the theory of spontaneous generation&#46;</p><p id="par0195" class="elsevierStylePara elsevierViewall">B&#225;guena Cervellera<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> also describes an 1898 textbook on basic microbiology for students of human and veterinary medicine by Luis del R&#237;o y Lara&#44; professor of histology at the university in Saragossa&#46; Del R&#237;o y Lara&#39;s book was the first to present microbiology as a discipline separate from pathology&#46; A considerable number of pages in this book were devoted to 3 diseases relevant to dermatology&#58; actinomycosis&#44; tuberculosis in its various forms&#44; and leprosy&#46; Cocci &#40;staphylococci&#44; streptococci&#44; tetrads&#44; and sarcinae&#41; were mentioned in relation to phlegmon- and abscess-causing pyodermatitis&#46; Del R&#237;o y Lara also studied lymphangitis&#44; erysipelas &#40;which he referred to as streptococcal dermatitis of Fehleisen&#41; and carbuncles&#46; Syphilis&#44; trypanosomiasis &#40;<span class="elsevierStyleItalic">durina</span>&#41;&#44; and gonococcal infection were also given ample treatment&#46; Del R&#237;o y Lara customarily used a glycerinated gel-forming broth that already contained agar-agar and resembled the media used in microbiology today&#46;</p><p id="par0200" class="elsevierStylePara elsevierViewall">Jos&#233; Eugenio de Olavide published a short paper on <span class="elsevierStyleItalic">Vibrio cholerae</span><a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">13</span></a> in 1884&#44; the same year Robert Koch described this bacterium&#46; This publication apparently bore no relation to his practice of dermatology&#46; He was working at the laboratory of Hospital San Juan de Dios&#44; which belonged to the provincial government of Madrid &#40;the Diputaci&#243;n&#41;&#46; This hospital was a center of reference in the effort to control the cholera epidemics raging in Spain at the end of the 19th century&#46;</p></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Plant and Animal Parasitism</span><p id="par0205" class="elsevierStylePara elsevierViewall">Dermatology played an important role in helping the concept of contagion take root in medicine because lice infestation and scabies provided the paradigm for parasitic diseases in general and for disease transmission&#46; It was reasoned that if lice and mites could pass from one individual to another and cause disease&#44; even smaller parasites that were as yet unidentified could also do so&#46;</p><p id="par0210" class="elsevierStylePara elsevierViewall">Jos&#233; Eugenio de Olavide&#39;s atlas of anatomy contained a magnificent illustration of the <span class="elsevierStyleItalic">Sarcoptes scabiei</span> mite and explained the transmission of scabies&#46;</p><p id="par0215" class="elsevierStylePara elsevierViewall">B&#225;guena Cervellera<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> also notes the gradual acceptance of the idea that plants&#8212;fungi to be precise&#8212;could sometimes cause disease after Agostino Bassi demonstrated&#44; around 1830&#44; that a fungus was responsible for muscadine in silk worms&#46; Just such a fungal disease provides the starting point for the story in Alessandro Baricco&#39;s recent successful novel&#44; <span class="elsevierStyleItalic">Silk</span>&#46;</p><p id="par0220" class="elsevierStylePara elsevierViewall">The first studies of parasitic plants were done by botanists&#46; Johann Sch&#246;nlein&#44; Jacob Henle&#44; and Ernest Bazin were outstanding contributors to this branch of knowledge in the middle decades of the 19th century&#59; other names associated with parasitic plant studies included Gruby&#44; Richter&#44; Audouin&#44; Vogel&#44; and Lebert&#46;<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a> In the previously mentioned monograph on cholera by Olavide&#44;<a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">13</span></a> it is interesting that he referred to the bacterial pathogen as a &#8220;microscopic plant&#46;&#8221; The influence of botany on microbiology may surprise us today&#44; but even now our language reflects the ties between these disciplines&#44; as we continue to refer to microorganisms in general as germs&#46;</p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Historically Important Research Facilities</span><p id="par0225" class="elsevierStylePara elsevierViewall">Laboratories were&#8212;and continue to be&#8212;the places where microbiologists and dermatopathologists conduct their research&#46; Therefore&#44; it is essential to name the important research facilities as well as the relevant scientists&#46; B&#225;guena Cervellera<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> described them in her thesis&#46; One was the outstanding laboratory for the study of normal histology&#44; pathology&#44; and bacteriology founded in 1875 by Maestre de San Juan at the faculty of medicine in Madrid&#46; Another important laboratory in the same city was the National Institute of Hygiene and Bacteriology&#44; established in 1894&#46; This facility would grow into the larger National Institute of Serotherapy&#44; Vaccination and Bacteriology &#40;also called the Alfonso XIII National Institute of Hygiene&#41;&#44; which was created by Royal Decree on October 28&#44; 1899&#46; The laboratory at Madrid&#39;s military hospital&#8212;where Rafael Ariza and Jos&#233; Alabern worked&#8212;must also be mentioned&#46; That facility undertook both chemical and microscopic studies&#46; Finally&#44; the laboratory of Hospital de San Juan de Dios mentioned earlier will merit special attention in a separate section below&#46;</p><p id="par0230" class="elsevierStylePara elsevierViewall">The city of Barcelona created its own municipal laboratory in 1887&#46; Also noteworthy in the same city were the Ferr&#225;n Institute &#40;where Jaime Ferr&#225;n did his work&#41; and the Institute of Biology &#40;where Ram&#243;n Turr&#243; worked&#41;&#46;<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a></p></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Microscopy and Histochemistry&#58; Microbiology&#44; Pathology&#44; and Clinical Biochemistry</span><p id="par0235" class="elsevierStylePara elsevierViewall">Given our current notion of microbiology&#44; pathology&#44; and clinical biochemistry as distinct disciplines&#44; it seems striking to us today that the early microscopists worked simultaneously in all these branches of knowledge&#46; Microbiology and pathology were thrown together for a simple reason&#58; they used the same tool&#8212;the microscope&#8212;as well as similar chemicals for analysis and staining&#46; The term microscopy &#40;or the contemporary alternative micrography&#41; originally encompassed both those disciplines and was widely used inclusively to refer to them at the end of the 19th century&#46; The link to clinical biochemistry may seem more tenuous&#44; but in fact this field was once called histochemistry&#44; a term that has been recycled in recent decades with a completely new meaning&#46; The connection was that the microscopy laboratory was also the place for performing chemical tests&#46; Some contemporary dermatology textbooks referred to technicians in all these fields simply as laboratory men&#46;</p><p id="par0240" class="elsevierStylePara elsevierViewall">The end of the 19th century saw lines drawn between microscopy as practiced by pathologists on the one hand and microbiologists on the other&#46; It was Lu&#237;s del R&#237;o y Lara&#44; mentioned earlier&#44; who brought about this change&#46;</p><p id="par0245" class="elsevierStylePara elsevierViewall">In dermatology&#44; however&#44; the so-called laboratory men would continue to have broad areas of practice&#44; working as pathologists&#44; microbiologists&#44; and mycologists while at the same time performing serology and other analyses&#46;</p></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Microscopy&#44; Microphotography&#44; and Pathologists as Draftsmen</span><p id="par0250" class="elsevierStylePara elsevierViewall">The distinction between microscopy and microphotography &#40;also termed photomicrography&#41; should be underlined&#46; The earliest photoengravings of histologic sections date from the beginning of the 20th century&#44; and the first volume of the journal <span class="elsevierStyleItalic">Actas Dermo-Sifiliogr&#225;ficas</span> &#40;1909&#8211;1910&#41; contained several such illustrations&#46;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">15</span></a> Microphotography was a bit of a luxury&#44; however&#44; and early researchers did not routinely use it&#46; This meant that they were obliged to develop competence as draftsmen so they could accurately represent what they saw through their microscopes&#46; Contreras Rubio<a class="elsevierStyleCrossRef" href="#bib0005"><span class="elsevierStyleSup">1</span></a> noted that drawing remained a valued skill until very recently&#46; The cultivation of this avocation&#8212;or even essential craft&#8212;meant that certain classical European dermatopathologists&#44; such as Felix Pinkus and Achille Civatte&#44; became fine draftsmen who left us curious caricatures and miniature works of art in their laboratory notebooks and manuscripts&#46;</p></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">The Olavides&#58; Father and Son</span><p id="par0255" class="elsevierStylePara elsevierViewall">Jos&#233; Eug&#233;nio de Olavide became familiar with the microscope while still a student&#46; As early as 1857&#44; when he summarized the case of a patient from whom his supervisor&#44; Manuel Soler&#44; had removed a tumor&#44; Olavide remarked that &#8220;the microscope revealed only globules of fat&#46;&#8221; Mentioned earlier was Olavide and Federico Rubio Gal&#237;&#8217;s analysis of the air in the wards under Dr Mart&#237;n de Pedro to see if it contained potentially pathogenic microorganisms&#59; that collaboration led to the 1872 paper by Rubio Gal&#237;&#46;<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">12</span></a></p><p id="par0260" class="elsevierStylePara elsevierViewall">On his own&#44; Olavide studied fungi under the microscope&#44; sometimes commenting on observations made at magnifications of &#215;350 and &#215;700&#46; However&#44; when it was necessary to prepare tissue and other pathologic samples for viewing through a microscope&#44; Olavide relied on Rubio Gal&#237;&#8217;s expertise&#46;</p><p id="par0265" class="elsevierStylePara elsevierViewall">Olavide made it clear in his publications that he was convinced that scabies&#44; filariasis&#44; and tinea were caused by living microorganisms&#46; He was also the most prominent promotor of the concept of plant and animal parasitism in Spanish dermatology at the time&#46; One of his most celebrated experiments&#8212;carried out with Rubio Gal&#237; and Benito Hernando Espinosa&#8212;was successfully transmitting tinea from a patient to 3 animals&#58; a mouse&#44; a cat&#44; and a dog&#46; The mouse died&#44; but the dog escaped&#44; running through the precincts of the hospital&#8212;according to Olavide&#39;s own account&#8212;until a member of the hospital&#39;s staff&#44; fearing he would himself be infected&#44; turned the animal loose on the street&#44; where it soon vanished&#46; Olavide&#39;s talk on his induction into the Royal National Academy of Medicine and several articles in a Spanish journal covering ophthalmology&#44; venereology&#44; dermatology&#44; and urologic diseases &#40;<span class="elsevierStyleItalic">Revista Especial de Oftalmolog&#237;a&#44; Sifiliograf&#237;a&#44; Dermatolog&#237;a y Afecciones Urinarias</span>&#41; also dealt with plant and animal parasitism&#46; He argued that contagion was a fundamental concept of pathogenesis&#44; naming 3 types&#58; parasitic&#44; miasmatic&#44; and virulent&#46; Scabies and mycoses provided paradigms for the first type&#44; miasmatic contagion&#44; as Olavide understood it&#44; was related to fermentation&#46; Finally&#44; in virulent contagion&#44; the infectious agent would be a fluid &#40;of a purulent or serous type&#41;&#46;</p><p id="par0270" class="elsevierStylePara elsevierViewall">Jos&#233; Olavide Malo&#44; Jos&#233; Eugenio&#39;s son&#44; was also a dermatologist and a founding member of the Spanish Society of Dermatology&#46; Mentioned earlier was Olavide Malo&#39;s doctoral thesis on <span class="elsevierStyleItalic">Trichophyton schoenleinii</span>&#8212;classified as <span class="elsevierStyleItalic">Achorion schoenleini</span> at that time&#46;</p></span><span id="sec0145" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Antonio Mendoza and the Laboratory at Hospital de San Juan de Dios in Madrid</span><p id="par0280" class="elsevierStylePara elsevierViewall">Jos&#233; Eugenio de Olavide was the driving force behind the establishment of the laboratory at Hospital de San Juan de Dios in its first location &#40;Plaza de Ant&#243;n Mart&#237;n&#41; and he served as its head&#44; but the true star of this enterprise was one Antonio Mendoza &#40;<a class="elsevierStyleCrossRef" href="#fig0055">Fig&#46; 11</a>&#41;&#44; who must not be confused with Antonio Mendoza y Rueda&#44; professor of surgery at the University of Barcelona&#46; Mendoza the microscopist &#40;1848&#8211;1917&#41; worked more in microbiology than dermatopathology&#44; and he was particularly expert in methods for isolating and observing germs&#46; It was Olavide who brought Mendoza from Cadiz to Hospital de San Juan de Dios&#44; advancing much of the cost of Mendoza&#39;s work out of his own pocket&#44; though he was later reimbursed through funds from the provincial government of Madrid &#40;the Diputaci&#243;n&#41; through the accounts of the public charity hospital &#40;Beneficencia Provincial&#41;&#46;</p><elsevierMultimedia ident="fig0055"></elsevierMultimedia><p id="par0285" class="elsevierStylePara elsevierViewall">Mendoza was a highly skilled microbiologist and laboratory technician&#46; Some of his contemporaries&#8212;notably Rafael Ariza&#8212;considered him to have a weakness for comfort and luxury&#44; but this tendency is easy to understand if we remember the underlying vulnerability of his economic situation&#46; What is certain is that Mendoza&#39;s expertise yielded remarkable results in the laboratory&#46; Some decades later&#44; Sainz de Aja<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">16</span></a> recounted an anecdote&#44; possibly with slight exaggeration&#58; &#8220;When Dr Mendoza presided over the laboratory at Hospital de San Juan de Dios&#44; encouraged by Dr Olavide&#39;s progressive&#44; research-oriented spirit&#44; he made this suggestion&#58; &#8216;What do you think if we put up a sign saying&#44; &#8220;Discoveries made in this laboratory this week&#8221;&#63;&#8221;&#8217;</p><p id="par0290" class="elsevierStylePara elsevierViewall">This hospital laboratory had cutting edge equipment&#46; In 1880&#44; Maestre de San Juan even mentioned in passing that they had a device for projecting images of what they saw under the microscope<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a>&#58;</p><p id="par0295" class="elsevierStylePara elsevierViewall">&#8220;For classroom instruction in our department we use various types of microscopes to examine fresh specimens&#44; and we also have other fixed specimens in collections as well as atlases and line drawings for coloring&#46; In some sessions we use Dr Le Bon&#39;s device to project lantern slides&#46; For 8 years we have been using this projector&#44; which is similar to Molteni&#39;s&#44; and have added a microscope to the apparatus in a way that magnifies the slides without the preparations becoming damaged by heat&#46; The pharmacy department &#40;Professor Lleget&#41; has the latest model&#44; and there is another one at the histochemistry laboratory at Hospital de San Juan de Dios here in Madrid&#46;&#8221;</p><p id="par0300" class="elsevierStylePara elsevierViewall">Olavide also noted that Mendoza was commissioned by the authorities to travel abroad in 1884 to study <span class="elsevierStyleItalic">Vibrio cholerae</span> shortly after it was identified by Robert Koch&#46;<a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">13</span></a></p><p id="par0305" class="elsevierStylePara elsevierViewall">In 1899 a central health facility&#8212;the National Institute of Serotherapy&#44; Vaccination and Bacteriology&#44; also commonly referred to as the Alfonso XIII Institute&#8212;was founded by merging the National Institute of Hygiene and Bacteriology with the Central Institute of Vaccination&#46; Antonio Mendoza was probably associated with this new facility from the beginning or joined the staff soon after it was founded&#46; At the health spa in Cabreiro&#225;&#44; in the province of Ourense&#44; the visitor can find a plaque giving details of a chemical analysis of the waters&#44; dated 1905&#46; The plaque shows a report of an analysis signed that year by Mendoza in his capacity as member of the staff of the national institute in Madrid &#40;<a class="elsevierStyleCrossRef" href="#fig0060">Fig&#46; 12</a>&#41;&#46;</p><elsevierMultimedia ident="fig0060"></elsevierMultimedia><p id="par0310" class="elsevierStylePara elsevierViewall">Finally&#44; the historical sources that B&#225;guena Cervellera<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> analyzed in her doctoral thesis revealed that Mendoza was named as second coauthor on 6 publications&#44; more often than any other microbiologist except Jaime Ferr&#225;n&#46;</p></span><span id="sec0080" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Claudio Sala Pons</span><p id="par0315" class="elsevierStylePara elsevierViewall">Sala Pons merits a place of honor in the history of Spanish dermatopathology&#44; even if only for his collaboration with Juan de Az&#250;a in describing pseudoepithelioma &#40;vegetative pyoderma&#41;&#46; I have been unable to learn a great deal about his personal history&#44; but he was a follower of Ram&#243;n y Cajal&#44; working with him between 1892 and 1894&#46; In the master&#39;s recollections<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">17</span></a> there is mention of their collaboration&#44; which led to several titles by Sala Pons on the spinal cord of amphibians &#40;Barcelona&#44; 1890&#41;&#44; the cerebral cortex of birds &#40;1893&#41;&#44; and his thesis on the glial cells of vertebrates&#46; Ram&#243;n y Cajal reported that Sala Pons presented the thesis to a tribunal in Barcelona in 1894&#46;</p><p id="par0320" class="elsevierStylePara elsevierViewall">On October 8&#44; 1911&#44; the newspaper <span class="elsevierStyleItalic">ABC</span> announced a course taught by Sala Pons at the faculty of medicine in Madrid&#46; The initial lecture dealt with physical&#44; chemical and microscopy techniques with clinical applications&#44; including the reagents and equipment required by a laboratory&#46;<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">18</span></a> The announcement can now be found on the Internet&#46; The monograph on pseudoepithelioma coauthored with Juan de Az&#250;a lists Sala Pons&#39;s affiliations as the faculties of pharmacy and sciences&#59; specifically he was full professor in charge of microbiology at the faculty of pharmacy&#46; Another historical document that can be accessed on the Internet mentions Sala Pons&#39;s application for the directorship of the Museum of Natural Sciences in Barcelona in February 1921&#59; his candidacy was rejected on the grounds that he was more than 45 years old&#46;<a class="elsevierStyleCrossRef" href="#bib0095"><span class="elsevierStyleSup">19</span></a></p><p id="par0325" class="elsevierStylePara elsevierViewall">Sala Pons and de Az&#250;a worked closely together&#59; de Az&#250;a described a complicated case of cutaneous necrobiosis in a young male patient at an international medical conference in Madrid in 1903&#44; using slides prepared by Sala Pons&#46; The case report was published the following year with 2 excellent illustrations of the capillaries of the skin&#44; a venule&#44; and an arteriole&#46; For the same conference&#44; these men prepared another presentation on the possible microbial origin of eczema&#46; However&#44; it is their joint work on pseudoepitheliomas &#40;<a class="elsevierStyleCrossRef" href="#fig0065">Fig&#46; 13</a>&#41; that perhaps marks their main contribution to the literature&#44; as it is one of the first&#8212;if not the very first&#8212;descriptions of cutaneous pseudomalignancy&#46;</p><elsevierMultimedia ident="fig0065"></elsevierMultimedia></span><span id="sec0150" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Juan Sanchez Puente&#58; Pharmacists as Microbiologists</span><p id="par0335" class="elsevierStylePara elsevierViewall">Sanchez Puente&#44; an enigmatic microbiologist working in the early years of the 20th century&#44; became known for a monograph on the human leprosy bacillus published in 1915&#46;<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">20</span></a> At that time he was identified as having a PhD in pharmacy and had a post as pharmacist in the public charity hospital &#40;Beneficencia Provincial&#41; of Madrid&#46; He presented himself at the beginning of the book&#44; however&#44; as chief of the laboratory at Hospital de San Juan de Dios in the same city and called himself a follower of Dr Castro&#44; in whose microbiology laboratory he began his study of the leprosy bacillus&#46;</p><p id="par0340" class="elsevierStylePara elsevierViewall">Although we have little information about S&#225;nchez Puente&#44; he merits mention in the present article because he belonged to that outstanding group of pharmacists and biologists who have been working in our hospital laboratories for a century now&#44; even though we are often not aware that they initial trained in those disciplines&#46;</p></span><span id="sec0085" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">Pathology and Microbiology in Catalan Dermatology&#58; Juan Gin&#233; Partag&#225;s and Jos&#233; Vi&#241;eta Bellaserra</span><p id="par0345" class="elsevierStylePara elsevierViewall">Juan Gin&#233; Partag&#225;s &#40;<a class="elsevierStyleCrossRef" href="#fig0070">Fig&#46; 14</a>&#41; was a prominent figure at the start of Spanish dermatology&#44; working in the field nearly as early as Olavide&#46; His main accomplishment in microscopy was the translation of a 19th-century classic&#44; Rudolf Virchow&#39;s great work on cellular pathology&#46; Marco Cu&#233;llar<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> notes that Dr Bartolomeu Robert also worked on the translation&#44; which was published by the journal <span class="elsevierStyleItalic">El Pabell&#243;n M&#233;dico&#46;</span> In 1871 Gin&#233; Partag&#225;s was named professor of surgery at the faculty of medicine in Barcelona&#44; succeeding Mendoza y Rueda&#46; In the first stage of his professorship his interest centered on normal histology&#46; His work in clinical histology came later&#46; Marco Cu&#233;llar<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> notes that his interest in psychiatry toward the end of his life distanced Gin&#233; Partag&#225;s from histology&#46; Several beautiful engravings illustrating the histologic structure of the skin appeared at the end of his book on surgical dermatology&#44; which also contained clinical photographs&#46;</p><elsevierMultimedia ident="fig0070"></elsevierMultimedia><p id="par0350" class="elsevierStylePara elsevierViewall">Another Barcelona dermatologist&#44; Jos&#233; Vi&#241;eta Bellaserra&#44; was born in Havana in 1857&#46; While an undergraduate&#44; he studied with Gin&#233; Partag&#225;s but later worked with Olavide on his doctorate&#44; which could only be earned in Madrid at that time&#46; Vi&#241;eta Bellaserra continued his studies in Paris &#40;H&#244;pital Saint-Louis&#41;&#44; returning to Barcelona to work in 1882&#46; That same year he published a study on cutaneous diphtheria in the journal <span class="elsevierStyleItalic">Revista de Ciencias M&#233;dicas</span>&#44; and in 1883 there was a paper describing 5 cases of cutaneous lymphadenia&#44; or Alibert mycosis fungoides&#46; Each was accompanied by histopathologic analysis&#46; Four of the cases were from the time he spent in Paris and the fifth was from Barcelona&#46;</p></span><span id="sec0090" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0120">Santiago Ram&#243;n y Cajal and P&#237;o del R&#237;o Hortega</span><p id="par0355" class="elsevierStylePara elsevierViewall">It would be unpardonable to leave the father of Spanish histology out of this account&#44; although it is true that Ram&#243;n y Cajal&#39;s own contributions to the study of the skin&#44; or dermatopathology&#44; were scarce or nil&#46; He focused his attention so specifically on nerve tissues&#8212;about which he made so many magnificent discoveries&#8212;that he practically ignored pathology and histology unrelated to the nervous system&#46; However&#44; some of his followers eventually contributed significantly to Spanish dermatopathology&#44; so we can say that he did influence this discipline&#44; but more through his creation of a school of practice than through direct contributions&#46; His new staining methods were influential to a certain degree and were applied to skin samples&#46;</p><p id="par0360" class="elsevierStylePara elsevierViewall">Another figure to mention is P&#237;o del R&#237;o Hortega&#46; Interestingly&#44; he appeared in 1909 as a founding member of the Spanish Society of Dermatology &#40;later&#44; the Academy&#41;&#46; His residence was in Valladolid&#44; and he was therefore listed as a supernumerary member &#40;<span class="elsevierStyleItalic">corresponsal nacional</span>&#41;&#44; a category reserved for those who did not live in the capital&#46; Although there are no signs that del R&#237;o Hortega participated actively in the sessions of the Society or that he ever published in <span class="elsevierStyleItalic">Actas Dermo-Sifiliogr&#225;ficas</span>&#44; his name appeared regularly on the annual roster of members&#46;</p></span><span id="sec0095" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0125">Dermatologists&#44; Pathologists&#44; and Microbiologists of the 1920s and 1930s</span><p id="par0365" class="elsevierStylePara elsevierViewall">The 1920s and 1930s were a period of excellence in the history of Spanish science&#46; Authors like Nicol&#225;s Calv&#237;n&#44; Guillermo de la Rosa King&#44; and Lorenzo Ruiz de Arcaute kept alive the ties between dermatology and the laboratory bench during this time&#46; That all three were serologists reflected the significance of these techniques in venereology after Wasserman&#39;s discoveries at the turn of the century&#46; Both de la Rosa and Ruiz de Arcaute were followers of Jorge Francisco Tello&#44;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a> although Fern&#225;ndez de la Portilla<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">22</span></a> described Ru&#237;z de Arcaute as a direct disciple of Ram&#243;n y Cajal&#46;</p><p id="par0370" class="elsevierStylePara elsevierViewall">De la Rosa was chief of the pathology department at Hospital de la Princesa de Madrid&#44; and he also worked at the venereal disease clinic of Juan de Az&#250;a &#40;Dispensario Az&#250;a&#41;&#46; An obituary in <span class="elsevierStyleItalic">Actas Dermo-Sifiliogr&#225;ficas</span> in 1944 noted that he had studied in New York&#44; thus forging a trail that would be taken by the long and brilliant list of professionals who have since traveled across the Atlantic to complete their training&#46; When Tello won the post of professor of histology and pathology at the faculty of medicine in 1926&#44; Ruiz de Arcaute took up Tello&#39;s post at the Hospital Cl&#237;nico de San Carlos&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a> He was also connected with the Alfonso XIII Institute&#44; according to de la Portilla&#46;<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">22</span></a> Both men worked with Mart&#237;nez Anido in a clinic to treat venereal diseases in Madrid&#44; on Calle de Sandoval&#44; where the offices of the Spanish Academy of Dermatology and Venereology &#40;AEDV&#41; were located for several decades&#46; De la Portilla was director of the clinic and Ruiz de Arcaute headed the laboratory&#46; They taught several courses on syphilis&#44; as we know from announcements in dermatology journals of the 1930s&#46; In a 1919 issue of <span class="elsevierStyleItalic">Actas Dermo-Sifiliogr&#225;ficas</span>&#44; Ru&#237;z de Arcaute<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a> published an article with interesting additional information on pathologic aspects of a case report by Enrique &#193;lvarez Sainz de Aja that had appeared earlier in the same journal&#46; The subject was sclerosis and erythema affecting the fingers&#46; Ru&#237;z de Arcaute died in 1936 when Franco&#39;s forces bombed Madrid during the Spanish Civil War&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a> These 3 men&#44; who died too soon&#44; were part of a promising generation of laboratory dermatologists&#46;</p><p id="par0375" class="elsevierStylePara elsevierViewall">Another small group of dermatologists carried out their own microbiologic and pathology studies&#46; One was Vicente Gimeno&#44; assistant professor of dermatology with Juan de Az&#250;a at the medical faculty in Madrid&#46; In the early years of <span class="elsevierStyleItalic">Actas Dermo-Sifiliogr&#225;ficas</span>&#44; he authored several papers describing his own dermatopathologic research&#46; In a paper on multiple subcutaneous hydatid cysts&#44;<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">24</span></a> he set out to discuss &#8220;the first slides we prepared for observation under the microscope as well as the photographs taken in the general pathology laboratory of the faculty of medicine&#46;&#8221; And in fact the text is accompanied by more than 36 interesting photoengravings&#46; Contemporary accounts also describe Jos&#233; Gay Prieto preparing his own material for histology at least occasionally&#46;<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">25</span></a></p><p id="par0380" class="elsevierStylePara elsevierViewall">Jos&#233; S&#225;nchez-Covisa and Julio Bejarano&#44; who carried on the work of Juan de Az&#250;a&#44; presented an interesting study &#40;in French&#41; in Strasbourg in 1923&#46; They had stained skin samples with the same silver nitrate method Ram&#243;n y Cajal and his followers had put to such good use for the study of nerves&#46; They did not clarify whether they or their associates at Hospital de San Juan de Dios prepared the samples&#46; S&#225;nchez-Covisa had not yet acceded to the professorship in dermatology he would earn later and through which he would create a strong laboratory under the direction of Manuel Hombr&#237;a &#205;&#241;iguez&#46; Stains based on a variety of silver salts must still have seemed novel and attractive in the 1940s&#46; In 1943 Xavier Vilanova and Juan Rubi&#243; Roig<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">26</span></a> published a modification of del R&#237;o Hortega&#39;s method for staining epithelial fibrils&#46;</p></span><span id="sec0100" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0130">The Civil War&#58; Purges and the Case of Manuel Hombr&#237;a &#205;&#241;iguez</span><p id="par0385" class="elsevierStylePara elsevierViewall">The case of Hombr&#237;a &#205;&#241;iguez offers an example of how lives were tragically disrupted by the Spanish Civil War&#44; as it affected the population in general and dermatology in particular&#46; Hombr&#237;a &#205;&#241;iguez&#39;s brother Antonio&#8212;also a dermatologist&#8212;was executed by Franco&#39;s troops when they took Cordoba in 1936&#44; and as Hombr&#237;a &#205;&#241;iguez himself was not allowed to work in the public health system or in education&#44; the years following the war were difficult&#46; On December 19&#44; 1940&#44; at the College of Physicians in Madrid&#44; he was declared unfit for posts that required public trust and was not rehabilitated politically until February 8&#44; 1946&#46; His death in 1953 was noted in <span class="elsevierStyleItalic">Actas Dermo-Sifiliogr&#225;ficas</span>&#46; A large number of young physicians who trained in Madrid in the 1930s suffered the same fate and professional ostracism as Hombr&#237;a &#205;&#241;iguez&#46; Among those most grievously affected were those who had studied under Jos&#233; S&#225;nchez-Covisa&#8212;men like Serviliano Pineda&#44; Covisa&#39;s assistant for dermatology and photomicroscopy starting in 1932&#59; Luis Vallejo Vallejo&#44; assistant instructor of serology in the dermatology laboratory&#59; and Emilio Enterr&#237;a Ga&#237;nza&#46;</p></span><span id="sec0105" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0135">Two Women in the History of Laboratory Dermatology in Spain</span><p id="par0390" class="elsevierStylePara elsevierViewall">Two women who worked in dermatology in Madrid and Barcelona in the 1930s were true pioneers in a field that was otherwise the exclusive province of men at the time&#46; One of them&#44; Mar&#237;a de Castro Cantalapiedra&#44; was named in one source as assistant professor for practical sessions under S&#225;nchez-Covisa&#59; however&#44; she was later named in purges and no further information about her has come to light&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> The second woman&#44; who worked in Barcelona&#44; was acknowledged as &#8220;Dr J&#46; Salvaus&#44;&#8221; the supplier of the anatomic and bacteriologic studies for a 1935 report in <span class="elsevierStyleItalic">Actas Dermo-Sifiliogr&#225;ficas</span> on a case of pseudopelade of Brocq&#44; authored by Xavier Vilanova&#46;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">28</span></a></p></span><span id="sec0110" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0140">The Last of the Old Generation&#58; Julio Rodr&#237;guez Puchol and Juan Rubi&#243; Roig</span><p id="par0395" class="elsevierStylePara elsevierViewall">The immediate predecessors of today&#39;s dermatopathologists are 2 outstanding figures&#58; Julio Rodr&#237;guez Puchol in Madrid and Juan Rubi&#243; Roig in Barcelona&#46; Contreras Rubio<a class="elsevierStyleCrossRef" href="#bib0005"><span class="elsevierStyleSup">1</span></a> mentioned them with respect&#46; Rodr&#237;guez Puchol was a follower of Tello and later worked with Ram&#243;n Mart&#237;nez in the Hospital del Rey&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a> P&#233;rez Pe&#241;a<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">29</span></a> mentions Rodr&#237;guez Puchol in his book on physicians who were exiled or purged from the medical faculty in Madrid&#44; although evidence to confirm the report is lacking&#46; According to the author&#44; Rodr&#237;guez Puchol was born in Valladolid on April 23&#44; 1912&#44; studied medicine in Madrid in the academic years of 1928&#8211;1929 and 1934&#8211;1935&#46; His grades were good and he was a student intern in pathology&#46; He graduated with a medical degree on March 23&#44; 1942&#44; receiving his diploma in September 1943 and his PhD in 1960&#44; with a thesis on the histopathology of cutaneous angiitis completed under the guidance of Fernando de Castro&#46; He completed specialist training in histopathology and clinical biochemistry in Valladolid in 1965&#46; As early as 1945&#44; Rodr&#237;guez Puchol<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">30</span></a> authored a publication in which his affiliation was given as head of the laboratory in the service of Enrique &#193;lvarez Sainz de Aja at Hospital de San Juan de Dios&#46; In that same academic year of 1945&#8211;1946&#44; he was listed as a member of the AEDV residing in Madrid &#40;number 7&#44; Calle de Campomanes&#41;&#46; Gay Prieto designated him professor of histopathology in the professional studies program in dermatology under his direction at the university&#46; With Contreras Due&#241;as he served as assistant secretary of the Sixth International Conference of Leprology held in Madrid in 1953 and was then named professor of histopathology in courses on leprology given at the sanatorium in Fontilles&#44; Alicante&#44; under the direction of Gay Prieto and Contreras Due&#241;as&#46;</p><p id="par0400" class="elsevierStylePara elsevierViewall">F&#233;lix Contreras Rubio described Rodr&#237;guez Puchol as follows in the inaugural talk at the Twenty-Second Conference of the Spanish Society of Pathology &#40;SEAP&#41; in Palma de Mallorca in 2005&#58; &#8220;He was a very well rounded general pathologist with specialist knowledge of infectious diseases&#44; endocrinology&#44; cardiology&#44; cytology&#44; and of course dermatology&#46; He served on the Board of Directors of the Spanish Academy of Dermatology for years and was founder and first treasurer of our own Spanish Society of Pathology&#46; Intelligent&#44; hard-working&#44; cultivated&#44; pleasant&#44; and an excellent teacher&#44; he mentored many aspiring pathologists in those early 1960s&#46;&#8221; Rodr&#237;guez Puchol served as secretary of the AEDV in the 1960s&#46;</p><p id="par0405" class="elsevierStylePara elsevierViewall">Juan Rubi&#243; Roig was another great name in Spanish dermatopathology in the years after the Civil War&#46; According to Xavier Sierra Valent&#237;&#44; who kindly furnished me with a few personal details&#44; Rubi&#243; Roig was born on December 16&#44; 1917&#44; and died on January 21&#44; 2003&#46; His father&#44; Joan Rubi&#243; i Bellver&#44; was an important Modernista architect who numbered among the successors of Gaud&#237;&#46; Rubi&#243; Roig finished his undergraduate medical studies in 1934&#46; He began working as a teaching assistant at the University of Barcelona in 1965&#46;</p><p id="par0410" class="elsevierStylePara elsevierViewall">Contreras Rubio also provided a vivid portrait of Rubi&#243; Roig in his opening talk at the SEAP conference&#58; &#8220;Joan Rubi&#243; was not much interested in presentations&#44; publications&#44; conferences&#44; or public acts of any type&#46; He nonetheless played a significant role in the international courses on surgical pathology organized by Lorenzo Galindo in Barcelona&#46; He was a wonderful person&#44; considerate and accomplished&#46; His many talents included an artistic inclination that he expressed through painting&#46; He was also inventive and designed his own tools&#44; such as a small microtome for surgical biopsies&#46; Another device was an apparatus that automatically embedded samples in paraffin&#46; Manufactured by Myr&#44; S&#46; L&#46; in Barcelona&#44; this processor was the first to be used in Spain when paraffin embedding was just becoming a part of laboratories&#8217; routine practice&#46; In that unforgettable laboratory at the department of dermatology in Barcelona&#44; many generations of dermatologists were taught as well as all the pathologists who chose to spend shorter or longer periods learning from him&#46;&#8221;</p><p id="par0415" class="elsevierStylePara elsevierViewall">Rubi&#243; Roig&#39;s career was spent largely in the shadow of Xavier Vilanova&#44; whom he accompanied to posts at the faculties in Valladolid&#44; Valencia&#44; and Barcelona&#46; In some of the papers he authored alone&#44; his credentials were given as departmental histopathologist&#46; In Valencia&#44; he worked alongside Jos&#233; Esteller for a time&#46; Rubi&#243; Roig&#39;s name first appeared on the roster of the AEDV in Volume 36 &#40;1944&#8211;1945&#41;&#44; when he was said to reside at number 190 on Via Layetana in Barcelona&#46;</p></span><span id="sec0115" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0145">From the Age of Pioneers to the Present</span><p id="par0420" class="elsevierStylePara elsevierViewall">At the beginning of the 20th century&#44; clinical biochemistry&#8212;especially serology&#8212;and microbiology began to take paths that led them to diverge somewhat from dermatology&#46; The ties between mycology and dermatology&#44; on the other hand&#44; remained close&#46; Dermatologists played notable roles in this field in the 1930s and 1940s&#46; Important were Eduardo de Gregorio in Saragossa in the 1930s and 1940s&#44; and Manuel Pereiro Cuesta and Manuel Pereiro Migu&#233;ns &#40;father and son&#41; in Santiago de Compostela&#46;</p><p id="par0425" class="elsevierStylePara elsevierViewall">Names in the recent history of dermatopathology are much more familiar to us than those mentioned earlier in this article&#46; Some&#44; like Abelardo Moreno&#44; an outstanding pathologist&#44; and Adolfo Aliaga&#44; a superb dermatologist&#44; are no longer with us&#46; They&#44; along with their generation of mentors in America and other European countries&#44; are the ones who trained the young&#44; rising group of dermatopathologists who work beside us in our laboratories today&#46;</p></span><span id="sec0120" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0150">Ethical Disclosures</span><span id="sec0125" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0155">Protection of human and animal subjects</span><p id="par0430" class="elsevierStylePara elsevierViewall">The author states that no experiments were performed on humans or animals for this investigation&#46;</p></span><span id="sec0130" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0160">Confidentiality of data</span><p id="par0435" class="elsevierStylePara elsevierViewall">The author declares that he followed the protocols of his hospital concerning the publication of patient data&#44; and that all the patients mentioned were appropriately informed and gave their written informed consent&#46;</p></span><span id="sec0135" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0165">Right to privacy and informed consent</span><p id="par0440" class="elsevierStylePara elsevierViewall">The author declares that he obtained informed consent from the patients and&#47;or subjects referred to in this article&#46;</p></span></span><span id="sec0140" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0170">Conflicts of Interest</span><p id="par0445" class="elsevierStylePara elsevierViewall">The author declares that he has no conflicts of interest&#46;</p></span></span>"
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          "titulo" => "Sources of Information on Dermatology and the Specialty&#39;s Relation to Microbiology and Pathology"
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          "titulo" => "The Early Years of Microscopy&#58; Cris&#243;stomo Mart&#237;nez&#44; the First of the Spanish Microscopists"
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          "titulo" => "Earliest Microscopic Images of the Skin in the Spanish Medical Literature"
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          "titulo" => "Microscopy Arrives in 19th-Century Spain by Way of Paris"
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          "titulo" => "Aureliano Maestre de San Juan"
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          "titulo" => "Federico Rubio Gal&#237;"
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          "titulo" => "Salvador Cardenal"
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          "titulo" => "Some Contributions of Spanish Microbiology to Epidemiology"
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          "titulo" => "Plant and Animal Parasitism"
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          "titulo" => "Microscopy and Histochemistry&#58; Microbiology&#44; Pathology&#44; and Clinical Biochemistry"
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          "titulo" => "Microscopy&#44; Microphotography&#44; and Pathologists as Draftsmen"
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          "titulo" => "The Olavides&#58; Father and Son"
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          "titulo" => "Antonio Mendoza and the Laboratory at Hospital de San Juan de Dios in Madrid"
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          "titulo" => "Claudio Sala Pons"
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          "titulo" => "Juan Sanchez Puente&#58; Pharmacists as Microbiologists"
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          "titulo" => "Pathology and Microbiology in Catalan Dermatology&#58; Juan Gin&#233; Partag&#225;s and Jos&#233; Vi&#241;eta Bellaserra"
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          "titulo" => "Santiago Ram&#243;n y Cajal and P&#237;o del R&#237;o Hortega"
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          "titulo" => "Dermatologists&#44; Pathologists&#44; and Microbiologists of the 1920s and 1930s"
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          "titulo" => "The Civil War&#58; Purges and the Case of Manuel Hombr&#237;a &#205;&#241;iguez"
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          "titulo" => "Two Women in the History of Laboratory Dermatology in Spain"
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          "titulo" => "The Last of the Old Generation&#58; Julio Rodr&#237;guez Puchol and Juan Rubi&#243; Roig"
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            1 => "Historia de la dermatopatolog&#237;a espa&#241;ola"
            2 => "Historia de la microbiolog&#237;a espa&#241;ola"
            3 => "Historia de la micolog&#237;a espa&#241;ola"
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        "titulo" => "Abstract"
        "resumen" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Cris&#243;stomo Mart&#237;nez from Valencia was a pioneering microscopist in 17th-century Europe&#46; The first microscopic representations of skin in Spain appeared in an 18th-century work by Mart&#237;n Mart&#237;nez&#46; Microbiology and histopathology progressed considerably in the late 19th century thanks to anatomists like Maestre de San Juan and surgeons like Federico Rubio Gal&#237;&#46; The first Spanish pathologist to specialize in dermatology was Antonio Mendoza&#44; a colleague of Jos&#233; Eugenio de Olavide at the Hospital San Juan de Dios in Madrid&#46; Claudio Sala and Juan de Az&#250;a also made significant contributions&#44; including the description of pseudoepithelioma&#46;</p><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Several disciples of Santiago Ram&#243;n y Cajal and Jorge FranciscoTello&#44; such as Lorenzo Ruiz de Arcaute and Guillermo de la Rosa King&#44; consolidated the dermatology laboratory&#44; but the Civil War sent many into exile or deprived them of their professional status&#46;</p><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Juan Rubi&#243; in Barcelona and Julio Rodr&#237;guez Puchol in Madrid were the immediate predecessors of today&#39;s dermatopathologists&#46;</p>"
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        "resumen" => "<p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">El valenciano Cris&#243;stomo Mart&#237;nez fue un microscopista pionero en Europa en el siglo <span class="elsevierStyleSmallCaps">xvii</span>&#46; Las primeras representaciones microsc&#243;picas cut&#225;neas en Espa&#241;a aparecen en una obra del siglo <span class="elsevierStyleSmallCaps">xviii</span> de Mart&#237;n Mart&#237;nez&#46;</p><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">La microbiolog&#237;a y la histopatolog&#237;a progresaron mucho a finales del siglo <span class="elsevierStyleSmallCaps">xix</span> gracias a anatomistas&#44; como Maestre de San Juan&#44; y cirujanos&#44; como Federico Rubio Gal&#237;&#46;</p><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">El primer dermatopat&#243;logo espa&#241;ol fue Antonio Mendoza&#44; colaborador de Jos&#233; Eugenio de Olavide en el Hospital de San Juan de Dios de Madrid&#46; Claudio Sala hizo importantes aportaciones con Az&#250;a&#44; como la descripci&#243;n de los pseudoepiteliomas&#46;</p><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Algunos disc&#237;pulos de Santiago Ram&#243;n y Cajal y Jorge Francisco Tello&#44; como Lorenzo Ruiz de Arcaute y Guillermo de la Rosa King&#44; consolidaron el laboratorio dermatol&#243;gico&#46; La Guerra Civil espa&#241;ola llev&#243; a muchos al exilio o a la depuraci&#243;n&#46;</p><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Juan Rubi&#243; en Barcelona y Julio Rodr&#237;guez Puchol en Madrid son los antecesores inmediatos de nuestros actuales dermatopat&#243;logos&#46;</p>"
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        "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Please cite this article as&#58; del R&#237;o E&#46; Los comienzos de la Dermatopatolog&#237;a y de la Microbiolog&#237;a dermatol&#243;gica en Espa&#241;a&#46; Actas Dermosifiliogr&#46; 2014&#59;105&#58;135&#8211;149&#46;</p>"
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          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Beautiful rendering of a primitive single-lens microscope&#46; This detail&#44; from an engraving by the Valencian microscopist Cris&#243;stomo Mart&#237;nez&#44; dates from the second half of the 17th century&#46;</p>"
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          "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Detail from a plate illustrating Mart&#237;n Mart&#237;nez&#39;s human anatomy text &#40;<span class="elsevierStyleItalic">Anatom&#237;a completa del hombre</span>&#41;&#46; This small figure is probably the first Spanish depiction of skin as seen under a microscope&#46;</p>"
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          "en" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Three-dimensional rendering of skin &#40;another detail from the same plate <a class="elsevierStyleCrossRef" href="#fig0010">Figure 2</a> was taken from&#41;&#46; Some features&#44; such as the branches on hairs&#44; are probably errors or the result of artistic license&#46;</p>"
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          "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">Histologic illustration of skin adnexa from the 1886 dermatology manual &#40;<span class="elsevierStyleItalic">&#193;lbum Cl&#237;nico de Dermatolog&#237;a</span>&#41; by Jer&#243;nimo P&#233;rez Ortiz&#44; published by the journal <span class="elsevierStyleItalic">Revista de Medicina y Cirug&#237;a Pr&#225;cticas</span>&#46; The illustrations were probably versions of those that appeared in other European anatomy textbooks of the period&#46;</p>"
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          "en" => "<p id="spar0065" class="elsevierStyleSimplePara elsevierViewall">Illustration of a Raspail-type microscope and other equipment used by Jos&#233; Mar&#237;a G&#243;mez Alam&#225; in practical classes&#46; This plate appeared in an interesting book by this author on dissection &#40;<span class="elsevierStyleItalic">Arte de disecar</span>&#41;&#59; this plate is from the second edition of 1872&#46;</p>"
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        "descripcion" => array:1 [
          "en" => "<p id="spar0070" class="elsevierStyleSimplePara elsevierViewall">Portrait of Aureliano Maestre de San Juan&#44; the first and best example of the Spanish anatomists who pioneered the study of histology in the second half of the 19th century&#46; Maestre de San Juan was the most important histologist working in Spain before Ram&#243;n y Cajal&#46;</p>"
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          "en" => "<p id="spar0075" class="elsevierStyleSimplePara elsevierViewall">Illustration of a Nachet-type microscope&#44; taken from Maestre de San Juan&#39;s 1879 treatise on general anatomy &#40;<span class="elsevierStyleItalic">Tratado de anatom&#237;a general</span>&#41;&#46; This microscope was among those favored by Spanish authors of the 1870s and 1880s&#46;</p>"
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          "en" => "<p id="spar0080" class="elsevierStyleSimplePara elsevierViewall">Federico Rubio Gal&#237;&#44; an eminent surgeon and good friend of Jos&#233; Eugenio de Olavide&#46; The 2 men collaborated at various points in their careers&#46; This portrait was published in the Spanish anatomy journal edited by Pedro Gonz&#225;lez de Velasco&#58; <span class="elsevierStyleItalic">Anfiteatro Anat&#243;mico Espa&#241;ol</span>&#46;</p>"
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          "en" => "<p id="spar0085" class="elsevierStyleSimplePara elsevierViewall">Detail of an illustration in Jos&#233; Eugenio de Olavide&#39;s atlas of anatomy&#44; showing a liver with syphilitic gummas found in an autopsy performed by Federico Rubio Gal&#237;&#46; The histologic section of the lesion is shown above the liver&#46;</p>"
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          "en" => "<p id="spar0090" class="elsevierStyleSimplePara elsevierViewall">Color lithograph from Jos&#233; Eugenio de Olavide&#39;s atlas of anatomy&#44; showing an ulcerated&#44; tuberous lesion on the upper eyelid treated by the surgeon Federico Rubio Gal&#237;&#46; The fine lines drawn around the eye suggest the flap used to repair the defect&#46; On the right is a rendering of the microscopic view of the histologic specimen prepared by Rubio Gal&#237;&#46; It is described as &#8220;embryonic tissue&#58; epithelial cells in various stages of formation&#46;&#8221;</p>"
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          "en" => "<p id="spar0095" class="elsevierStyleSimplePara elsevierViewall">Caricature of Antonio Mendoza&#44; microscopist at Hospital de San Juan de Dios in Madrid&#44; published in a magazine of medical satire&#44; <span class="elsevierStyleItalic">El Doctor Sangredo</span>&#46; The egg Mendoza is hatching from is a reference to cytology&#44; and bacteria &#40;cocci&#44; rods&#44; and spirilla&#41; can be seen along the upper edge of the drawing&#46;</p>"
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          "en" => "<p id="spar0100" class="elsevierStyleSimplePara elsevierViewall">Plaque commemorating an analysis of the waters at the Cabeiro&#225; spa in Verin&#44; in the province of Ourense&#44; in 1905&#46; Antonio Mendoza signed the analysis in his capacity as a member of the staff of the Instituto Alfonso XIII&#44; directed by Santiago Ramon y Cajal&#46;</p>"
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          "en" => "<p id="spar0105" class="elsevierStyleSimplePara elsevierViewall">Page with figures in the monograph on pseudoepithelioma by Juan de Az&#250;a and Claudio Sala Pons&#44; illustrating this publication&#39;s most important contribution&#58; pseudoepitheliomatous hyperplasia&#46;</p>"
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          "en" => "<p id="spar0110" class="elsevierStyleSimplePara elsevierViewall">Portrait of Juan Gin&#233; Partag&#225;s in the publication <span class="elsevierStyleItalic">Anfiteatro Anat&#243;mico Espa&#241;ol</span>&#46; A physician with many talents who applied himself to dermatology&#44; surgery&#44; and psychiatry&#44; Gin&#233; Partag&#225;s published a book on dermatologic surgery that contained beautiful illustrations of the skin&#46;</p>"
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                      "titulo" => "Dermatopatolog&#237;a&#58; ayer&#44; hoy &#191;y ma&#241;ana&#63;"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
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                        "tituloSerie" => "Piel"
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                        "paginaInicial" => "463"
                        "paginaFinal" => "467"
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              "identificador" => "bib0010"
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                    0 => array:2 [
                      "titulo" => "&#191;Qui&#233;n gan&#243; el debate&#63; Reflexiones sobre el futuro de la Dermatopatolog&#237;a"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "J&#46; Basas-Vila"
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                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Actas Dermosifiliogr"
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                        "paginaInicial" => "749"
                        "paginaFinal" => "752"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19091212"
                            "web" => "Medline"
                          ]
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              "identificador" => "bib0015"
              "etiqueta" => "3"
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                    0 => array:2 [
                      "titulo" => "La morfolog&#237;a microsc&#243;pica normal y patol&#243;gica en la medicina del siglo <span class="elsevierStyleSmallCaps">xix</span> anterior a Cajal&#46; Tesis de doctorado"
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                    0 => array:1 [
                      "Libro" => array:3 [
                        "fecha" => "1966"
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                        "editorialLocalizacion" => "Valencia"
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              ]
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                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Ann Dermatol Veneorol"
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                      "titulo" => "Sobre el examen microsc&#243;pico del vapor atmosf&#233;rico de la enfermer&#237;a del Dr&#46; Mart&#237;n de Pedro en el Hospital General"
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                      "titulo" => "Tratamiento curativo y preservativo del c&#243;lera"
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                            0 => "&#40;JE&#41;&#46; Olavide"
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                      "Libro" => array:3 [
                        "fecha" => "1884"
                        "editorial" => "Imprenta y estereotipia de El Liberal"
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                ]
              ]
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              "identificador" => "bib0070"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Historia de las enfermedades producidas por hongos"
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                          "autores" => array:1 [
                            0 => "X&#46; Sierra"
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                    0 => array:1 [
                      "Libro" => array:3 [
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                        "editorial" => "MRA"
                        "editorialLocalizacion" => "Barcelona"
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                  ]
                ]
              ]
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            14 => array:3 [
              "identificador" => "bib0075"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Breve historia de <span class="elsevierStyleItalic">Actas Dermo-Sifiliogr&#225;ficas</span>"
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                          "etal" => false
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                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
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                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16796962"
                            "web" => "Medline"
                          ]
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                  ]
                ]
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              "identificador" => "bib0080"
              "etiqueta" => "16"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Discurso del presidente de la Academia&#46; Dr&#46; A Sainz de Aja&#46; Ante mi tercera elecci&#243;n para presidente"
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                    0 => array:1 [
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                        "paginaFinal" => "142"
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              "identificador" => "bib0085"
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                0 => array:1 [
                  "referenciaCompleta" => "Cajal&#62;Recuerdos de mi vida&#62;Sumario&#62;Ap&#233;ndice 2 &#91;cited 2012 Jan 31&#93;&#46; Available from&#58; <a id="intr0015" class="elsevierStyleInterRef" href="http://cvc.cervantes.es/ciencia/cajal/cajal_recuerdos/recuerdos/apendice_02.htm">http&#58;&#47;&#47;cvc&#46;cervantes&#46;es&#47;ciencia&#47;cajal&#47;cajal&#95;recuerdos&#47;recuerdos&#47;apendice&#95;02&#46;htm</a>"
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                  "referenciaCompleta" => "Academias y Centros&#46; Facultad de Medicina&#46; ABC&#44; domingo 8 de octubre de 1911&#46; p&#46; 12&#46; &#91;cited 2012 Jan 31&#93;&#46; Available from&#58; <a id="intr0020" class="elsevierStyleInterRef" href="http://hemeroteca.abc.es/nav/Navigate.exe/hemeroteca/madrid/abc/1911/10/08/012.html">http&#58;&#47;&#47;hemeroteca&#46;abc&#46;es&#47;nav&#47;Navigate&#46;exe&#47;hemeroteca&#47;madrid&#47;abc&#47;1911&#47;10&#47;08&#47;012&#46;html</a>"
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              "identificador" => "bib0095"
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                0 => array:1 [
                  "referenciaCompleta" => "Apendix IV&#46; Dictamen de la pon&#232;ncia nomenada per a informar sobre els merits dels concursants a la pla&#231;a de Director del Museu &#91;cited 2012 Jan 31&#93;&#46; Available from&#58; <a id="intr0025" class="elsevierStyleInterRef" href="http://bibdigital.rjb.csic.es/Imagenes/P1101_05/P1101_05_034.pdf">http&#58;&#47;&#47;bibdigital&#46;rjb&#46;csic&#46;es&#47;Imagenes&#47;P1101&#95;05&#47;P1101&#95;05&#95;034&#46;pdf</a>"
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Article information

ISSN: 15782190
Original language: English

DOI:10.1016/j.adengl.2014.01.001

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2024 November	17	11	28
2024 October	83	47	130
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2024 August	97	52	149
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2024 March	99	34	133
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2024 January	68	32	100
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2023 June	61	32	93
2023 May	73	28	101
2023 April	57	17	74
2023 March	62	30	92
2023 February	70	19	89
2023 January	58	21	79
2022 December	83	41	124
2022 November	73	24	97
2022 October	53	35	88
2022 September	60	44	104
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2022 July	68	36	104
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2022 April	85	35	120
2022 March	98	54	152
2022 February	84	35	119
2022 January	86	43	129
2021 December	64	49	113
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2021 October	68	45	113
2021 September	68	46	114
2021 August	56	32	88
2021 July	37	24	61
2021 June	47	34	81
2021 May	60	54	114
2021 April	55	35	90
2021 March	61	25	86
2021 February	37	33	70
2021 January	27	21	48
2020 December	37	11	48
2020 November	29	16	45
2020 October	31	10	41
2020 September	36	16	52
2020 August	27	16	43
2020 July	31	11	42
2020 June	41	23	64
2020 May	30	20	50
2020 April	36	28	64
2020 March	28	21	49
2020 February	5	9	14
2020 January	4	10	14
2019 December	8	4	12
2019 November	4	4	8
2019 October	8	3	11
2019 September	8	8	16
2019 August	4	12	16
2019 July	4	13	17
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2018 September	6	0	6
2018 July	0	1	1
2018 June	0	2	2
2018 May	0	9	9
2018 April	0	5	5
2018 March	0	2	2
2018 February	39	4	43
2018 January	44	3	47
2017 December	64	15	79
2017 November	45	15	60
2017 October	47	12	59
2017 September	51	14	65
2017 August	55	12	67
2017 July	44	19	63
2017 June	84	25	109
2017 May	71	15	86
2017 April	64	19	83
2017 March	59	33	92
2017 February	38	7	45
2017 January	34	13	47
2016 December	47	10	57
2016 November	86	15	101
2016 October	106	12	118
2016 September	181	17	198
2016 August	90	4	94
2016 July	49	9	58
2016 June	13	10	23
2016 May	4	5	9
2016 April	4	1	5
2016 March	6	24	30
2016 February	13	26	39
2016 January	8	15	23
2015 December	4	11	15
2015 November	9	5	14
2015 October	10	3	13
2015 September	2	1	3
2015 August	8	2	10
2015 July	121	2	123
2015 June	95	3	98
2015 May	84	3	87
2015 April	89	4	93
2015 March	115	3	118
2015 February	163	6	169
2015 January	126	3	129
2014 December	63	5	68
2014 November	68	4	72
2014 October	80	7	87
2014 September	101	3	104
2014 August	63	3	66
2014 July	110	6	116
2014 June	124	3	127
2014 May	83	10	93
2014 April	91	8	99
2014 March	53	4	57

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