Novelties in dermatology
Immunofluorescence Mapping for Diagnosis of Congenital Epidermolysis Bullosa
Mapeo por inmunofluorescencia para el diagnóstico de epidermólisis ampollosa congénita
R. Cepeda-Valdésa, G. Pohla-Gubob, J.R. Borbolla-Escobozaa, O. Barboza-Quintanac, J. Ancer-Rodríguezc, H. Hintnerb, J.C. Salas-Alanisad,
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a Centro de Investigación Clínica, Escuela de Biotecnología y Salud, Instituto Tecnológico y de Estudios Superiores de Monterrey, Monterrey, Mexico
b Department of Dermatology, Universidad Médica Privada de Paracelsus, Salzburg, Austria
c Servicio de Patología, Hospital Universitario “Dr. José E. González”, Universidad Autónoma de Nuevo León, Nuevo León, Mexico
d Departamento de Dermatología, Hospital Universitario “Dr. José E. González”, Universidad Autónoma de Nuevo León, Nuevo León, Mexico
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"apellidos" => "Cepeda-Valdés" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 1 => array:3 [ "nombre" => "G." "apellidos" => "Pohla-Gubo" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] 2 => array:3 [ "nombre" => "J.R." "apellidos" => "Borbolla-Escoboza" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 3 => array:3 [ "nombre" => "O." "apellidos" => "Barboza-Quintana" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] ] ] 4 => array:3 [ "nombre" => "J." "apellidos" => "Ancer-Rodríguez" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] ] ] 5 => array:3 [ "nombre" => "H." "apellidos" => "Hintner" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] 6 => array:4 [ "nombre" => "J.C." "apellidos" => "Salas-Alanisa" "email" => array:1 [ 0 => "drjuliosalas@gmail.com" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">¿</span>" "identificador" => "cor0005" ] ] ] ] "afiliaciones" => array:4 [ 0 => array:3 [ "entidad" => "Centro de Investigación Clínica, Escuela de Biotecnología y Salud, Instituto Tecnológico y de Estudios Superiores de Monterrey, Monterrey, Mexico" "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Department of Dermatology, Universidad Médica Privada de Paracelsus, Salzburg, Austria" "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] 2 => array:3 [ "entidad" => "Servicio de Patología, Hospital Universitario “Dr. José E. González”, Universidad Autónoma de Nuevo León, Nuevo León, Mexico" "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] 3 => array:3 [ "entidad" => "Departamento de Dermatología, Hospital Universitario “Dr. José E. González”, Universidad Autónoma de Nuevo León, Nuevo León, Mexico" "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Mapeo por inmunofluorescencia para el diagnóstico de epidermólisis ampollosa congénita" ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2010-03-11" "fechaAceptado" => "2010-06-10" "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec84799" "palabras" => array:4 [ 0 => "Epidermolysis bullosa" 1 => "Classification" 2 => "Antigen mapping" 3 => "Immunofluorescence" ] ] ] "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec84798" "palabras" => array:4 [ 0 => "Epidermólisis ampollosa" 1 => "Clasificación" 2 => "Mapeo antigénico" 3 => "Inmunofluorescencia" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "en" => array:2 [ "titulo" => "Abstract" "resumen" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">The tools for diagnosis of epidermolysis bullosa have advanced greatly since Hintner's group introduced antigen mapping as a diagnostic test for this family of genodermatoses. Monoclonal or polyclonal antibodies raised against some of the specific proteins found in the epidermis and basement membrane of the epidermis have allowed 4 types of epidermolysis bullosa de be identified and all variants to be classified. When a newborn baby presents with blisters, many conditions are implicated in the differential diagnosis. Examination under an optical microscope can suggest epidermolysis bullosa, but immunofluorescence mapping and electron microscopy are required for confirmation of the diagnosis and further classification of congenital epidermolysis bullosa. This article explains the importance of immunofluorescence antigen mapping and describes the methods employed for classification and subclassification of epidermolysis bullosa.</p>" ] "es" => array:2 [ "titulo" => "Resumen" "resumen" => "<p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Las herramientas para el diagnóstico en las epidermólisis ampollosas (EA) han tenido un gran avance desde que Hintner et al introdujeron el mapeo antigénico como prueba diagnóstica en este grupo de genodermatosis. La utilización de anticuerpos monoclonales/policlonales dirigidos contra algunas de las proteínas específicas que conforman la epidermis y la membrana basal epidérmica han servido para clasificar los 4 tipos de epidermólisis ampollosa y subclasificar todas sus variantes. Ante la presencia de un recién nacido con ampollas surgen diagnósticos diferenciales múltiples, en donde la microscopia de luz orienta el diagnostico de epidermólisis ampollosa. Sin embargo, el mapeo por inmunofluorescencia y la microscopia electrónica permiten confirmar y clasificar a las epidermólisis ampollosas congénitas.</p><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">En este artículo, se explica la importancia y metodología para desarrollar la técnica de mapeo antigénico por inmunofluorescencia, con el propósito de clasificar y subclasificar las epidermólisis ampollosas.</p>" ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:30 [ 0 => array:3 [ "identificador" => "bib1" "etiqueta" => "1." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Definition" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "C.M. Lanschuetzer" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "LibroEditado" => array:2 [ "titulo" => "Life with Epidermolysis Bullosa (EB)." 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| Year/Month | Html | Total | |
|---|---|---|---|
| 2024 November | 11 | 12 | 23 |
| 2024 October | 58 | 54 | 112 |
| 2024 September | 68 | 40 | 108 |
| 2024 August | 104 | 69 | 173 |
| 2024 July | 58 | 41 | 99 |
| 2024 June | 72 | 37 | 109 |
| 2024 May | 53 | 39 | 92 |
| 2024 April | 58 | 39 | 97 |
| 2024 March | 58 | 37 | 95 |
| 2024 February | 59 | 35 | 94 |
| 2024 January | 42 | 39 | 81 |
| 2023 December | 46 | 38 | 84 |
| 2023 November | 48 | 36 | 84 |
| 2023 October | 43 | 43 | 86 |
| 2023 September | 36 | 47 | 83 |
| 2023 August | 27 | 23 | 50 |
| 2023 July | 28 | 72 | 100 |
| 2023 June | 44 | 33 | 77 |
| 2023 May | 42 | 62 | 104 |
| 2023 April | 24 | 41 | 65 |
| 2023 March | 30 | 44 | 74 |
| 2023 February | 41 | 23 | 64 |
| 2023 January | 45 | 58 | 103 |
| 2022 December | 49 | 54 | 103 |
| 2022 November | 19 | 51 | 70 |
| 2022 October | 16 | 42 | 58 |
| 2022 September | 17 | 61 | 78 |
| 2022 August | 12 | 40 | 52 |
| 2022 July | 23 | 39 | 62 |
| 2022 June | 16 | 44 | 60 |
| 2022 May | 51 | 61 | 112 |
| 2022 April | 47 | 63 | 110 |
| 2022 March | 41 | 107 | 148 |
| 2022 February | 16 | 42 | 58 |
| 2022 January | 56 | 56 | 112 |
| 2021 December | 32 | 57 | 89 |
| 2021 November | 36 | 75 | 111 |
| 2021 October | 31 | 88 | 119 |
| 2021 September | 29 | 74 | 103 |
| 2021 August | 23 | 55 | 78 |
| 2021 July | 16 | 63 | 79 |
| 2021 June | 24 | 68 | 92 |
| 2021 May | 25 | 55 | 80 |
| 2021 April | 46 | 74 | 120 |
| 2021 March | 47 | 40 | 87 |
| 2021 February | 35 | 38 | 73 |
| 2021 January | 52 | 35 | 87 |
| 2020 December | 31 | 24 | 55 |
| 2020 November | 27 | 36 | 63 |
| 2020 October | 15 | 27 | 42 |
| 2020 September | 19 | 18 | 37 |
| 2020 August | 21 | 29 | 50 |
| 2020 July | 24 | 26 | 50 |
| 2020 June | 15 | 29 | 44 |
| 2020 May | 30 | 27 | 57 |
| 2020 April | 21 | 14 | 35 |
| 2020 March | 16 | 23 | 39 |
| 2020 February | 3 | 2 | 5 |
| 2020 January | 0 | 6 | 6 |
| 2019 December | 0 | 14 | 14 |
| 2019 November | 0 | 2 | 2 |
| 2019 October | 0 | 1 | 1 |
| 2019 September | 0 | 2 | 2 |
| 2019 August | 0 | 3 | 3 |
| 2019 June | 0 | 6 | 6 |
| 2019 May | 0 | 39 | 39 |
| 2019 April | 0 | 2 | 2 |
| 2019 March | 0 | 4 | 4 |
| 2019 February | 0 | 2 | 2 |
| 2018 February | 9 | 5 | 14 |
| 2018 January | 17 | 10 | 27 |
| 2017 December | 18 | 7 | 25 |
| 2017 November | 20 | 2 | 22 |
| 2017 October | 15 | 5 | 20 |
| 2017 September | 19 | 7 | 26 |
| 2017 August | 25 | 10 | 35 |
| 2017 July | 24 | 5 | 29 |
| 2017 June | 31 | 8 | 39 |
| 2017 May | 33 | 8 | 41 |
| 2017 April | 28 | 12 | 40 |
| 2017 March | 33 | 8 | 41 |
| 2017 February | 24 | 8 | 32 |
| 2017 January | 15 | 11 | 26 |
| 2016 December | 35 | 17 | 52 |
| 2016 November | 28 | 21 | 49 |
| 2016 October | 27 | 16 | 43 |
| 2016 September | 44 | 17 | 61 |
| 2016 August | 43 | 15 | 58 |
| 2016 July | 40 | 14 | 54 |
| 2016 June | 7 | 6 | 13 |
| 2016 May | 10 | 14 | 24 |
| 2016 April | 9 | 3 | 12 |
| 2016 March | 5 | 8 | 13 |
| 2016 February | 6 | 9 | 15 |
| 2016 January | 9 | 2 | 11 |
| 2015 December | 7 | 1 | 8 |
| 2015 November | 11 | 11 | 22 |
| 2015 October | 10 | 6 | 16 |
| 2015 September | 6 | 6 | 12 |
| 2015 August | 14 | 7 | 21 |
| 2015 July | 32 | 50 | 82 |
| 2015 June | 44 | 8 | 52 |
| 2015 May | 75 | 32 | 107 |
| 2015 April | 28 | 25 | 53 |
| 2015 March | 43 | 17 | 60 |
| 2015 February | 30 | 15 | 45 |
| 2015 January | 29 | 21 | 50 |
| 2014 December | 42 | 7 | 49 |
| 2014 November | 27 | 19 | 46 |
| 2014 October | 31 | 11 | 42 |
| 2014 September | 30 | 22 | 52 |
| 2014 August | 32 | 14 | 46 |
| 2014 July | 36 | 21 | 57 |
| 2014 June | 43 | 17 | 60 |
| 2014 May | 55 | 16 | 71 |
| 2014 April | 39 | 12 | 51 |
| 2014 March | 46 | 14 | 60 |
| 2014 February | 36 | 14 | 50 |
| 2014 January | 31 | 19 | 50 |
| 2013 December | 33 | 11 | 44 |
| 2013 November | 28 | 20 | 48 |
| 2013 October | 38 | 23 | 61 |
| 2013 September | 34 | 24 | 58 |
| 2013 August | 27 | 34 | 61 |
| 2013 July | 42 | 39 | 81 |
| 2013 June | 29 | 24 | 53 |
| 2013 May | 21 | 16 | 37 |
| 2013 April | 30 | 26 | 56 |
| 2013 March | 46 | 31 | 77 |
| 2013 February | 21 | 16 | 37 |
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