Biochemical and Biophysical Research Communications
Vaspin gene expression in human adipose tissue: Association with obesity and type 2 diabetes
Section snippets
Research design and methods
Subjects. Paired samples of visceral and subcutaneous adipose tissue were obtained from 196 Caucasian men (n = 98) and women (n = 98) who underwent open abdominal surgery for gastric banding, cholecystectomy, appendectomy, weight reduction surgery, abdominal injuries, or explorative laparotomy. The age ranged from 23 to 86 years and body mass index from 20.8 to 54.1 kg/m2. Thirty-six subjects had type 2 diabetes and 31 subjects had impaired glucose tolerance. All subjects had a stable weight with no
Visceral and subcutaneous vaspin mRNA expression
The analysis of 196 paired samples of visceral and subcutaneous adipose tissue revealed that vaspin mRNA expression was only detectable in 45 (23%) of the visceral and in 30 (15%) of the subcutaneous (SC) adipose tissue samples. None of the lean subjects (BMI < 25, n = 55) had detectable vaspin mRNA expression, whereas frequency of subjects with vaspin mRNA expression increased from overweight to obese individuals (Fig. 1). RT-PCR results were confirmed by Northern blot analysis in ten subjects
Discussion
Visceral adipose tissue-derived serpin (vaspin) was recently isolated as an adipocytokine from visceral white adipose tissue of Otsuka Long-Evans Tokushima fatty (OLETF) rats [12]. The OLETF rat is a model of type 2 diabetes, which is characterized by abdominal obesity, insulin resistance, hypertension, and dyslipidemia [18]. Vaspin was highly expressed in 30-week old rats, the age when obesity, body weight, and insulin levels peak in OLETF rats [12]. We studied vaspin mRNA expression in paired
Acknowledgments
This study was supported by grants from the Deutsche Forschungsgemeinschaft (DFG), BL 580/3-1 (to M.B.) and FA 376/3-1 (to M.F.) and the Bundesministerium für Bildung und Forschung (BMBF), Interdisciplinary Centre for Clinical Research (IZKF) at the University of Leipzig (Project N03) (to M.B.).
References (18)
- et al.
Contribution of intra-abdominal fat accumulation to the impairment of glucose and lipid metabolism in human obesity
Metabolism
(1987) Metabolic implication of body fat distribution
Diabetes Care
(1991)Visceral fat and insulin resistance-causative or correlative
Br. J. Nutri.
(2000)Subcutaneous and visceral adipose tissue: their relation to the metabolic syndrome
Endocr. Rev.
(2000)- et al.
A pilot study of long-term effects of a novel obesity treatment: omentectomy in connection with adjustable gastric banding
Int. J. Obes. Relat. Metab. Disord.
(2002) - et al.
Absence of an effect of liposuction on insulin action and risk factors for coronary heart disease
N. Engl. J. Med.
(2004) - et al.
Leptin secretion from subcutaneous and visceral adipose tissue in women
Diabetes
(1998) - et al.
Depot-related gene expression in human subcutaneous and omental adipocytes
Diabetes
(1998) - et al.
Production of plasminogen activator inhibitor 1 by human adipose tissue: possible link between visceral fat accumulation and vascular disease
Diabetes
(1997)