Original articleGene Expression Profiling and PRAME Status Versus Tumor-Node-Metastasis Staging for Prognostication in Uveal Melanoma
Section snippets
Clinical Data Collection
This retrospective cohort study was approved by the Institutional Review Board of the University of Miami School of Medicine. Patient information was accessed with proper informed consent and in accordance with the Health Insurance Portability and Accountability Act (HIPAA). The study included 240 patients with primary UMs arising from the choroid and/or ciliary body from the ocular oncology practice of J.W.H. The ocular pathology laboratory routinely provided cytologic verification of fine
Results
Among 240 consecutive patients diagnosed with UM arising from the choroid and/or ciliary body (Table 1), primary treatment consisted of I-125 plaque radiotherapy in 165 (68.8%) cases, enucleation in 74 (30.8%) cases, and observation in 1 case (0.4%). Tumor sample was obtained by fine needle aspiration biopsy in 166 (69.2%) and by postenucleation needle biopsy in 74 (30.8%). GEP was Class 1 in 128 (53.3%) cases and Class 2 in 112 (46.7%) cases. PRAME was positive in 83 (34.6%) cases, including
Discussion
In this study, we confirmed that GEP, PRAME, and TNM stage were each prognostic of metastasis in UM. Individually, GEP and PRAME both demonstrated prognostic accuracy that was superior to the TNM staging system. Combining GEP and PRAME into a 3-category model further enhanced the prognostic accuracy of this molecular classification system.
There are several limitations to the use of the TNM system for prognostication in UM. First, the TNM assigns increased metastatic risk to all UMs with LBD >
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2021, Biochimica et Biophysica Acta - Reviews on CancerCitation Excerpt :PRAME also enhances cancer cell migration, invasion, and metastasis [35]. Furthermore, overexpression of PRAME is closely associated with poor prognosis in various types of cancer [26,42–47]. The CT45 family consists of nine members which are located at the chromosome Xq26-q28 narrow region.
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2021, Seminars in Cancer BiologyCitation Excerpt :Retrospective studies have compared PRAME expression along with GEP and TNM staging [102,103]. They reported that GEP and PRAME showed a superior prognostic power than TNM staging, and that PRAME + status correlated with largest basal diameter, tumor volume, and worsening GEP class [102,103]. Another classification based on RNA expression, DNA methylation and chromosomal data from TCGA separated the UM primary tumors into categories A to D based on the presence of monosomy 3 and the degree of chromosome 8q gain [21,104].
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